##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AccI AccIII AgeI AscI AseI
AsnI AviII AvrII BbrPI BcgI
BciVI BclI BglI BglII BlnI
BsaAI BsaBI BseAI BseRI BsiWI
BsmI BspDI BspEI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI CpoI EagI EclXI
Eco52I EcoO65I EcoT22I FbaI FseI
FspI HpaI KspI MamI MfeI
MroI MunI NaeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RsrII SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 72 GACGTC GACGT^C
Acc65I 3020 GGTACC G^GTACC
AclI 324 AACGTT AA^CGTT
AflII 2549 CTTAAG C^TTAAG
AhdI 2864 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 344 AGCGCT AGC^GCT
Asp718I 3020 GGTACC G^GTACC
AspEI 2864 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2831 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2318 TGGCCA TGG^CCA
BamHI 932 GGATCC G^GATCC
BbeI 899 GGCGCC GGCGC^C
BfrI 2549 CTTAAG C^TTAAG
BlpI 4072 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 4072 GCTAAGC GC^T[ACGT]AGC
BsaWI 2724 ACCGGA [AT]^CCGG[AT]
BsiEI 1373 CGGTCG CG[GA][CT]^CG
Bsp1407I 3792 TGTACA T^GTACA
BspLU11I 3659 ACATGT A^CATGT
BspMI 983 GCAGGT ACCTGC(4/8)
BsrBI 50 GAGCGG CCGCTC(-3/-3)
BsrFI 3262 GCCGGT [GA]^CCGG[CT]
BsrGI 3792 TGTACA T^GTACA
BstXI 1994 CCACCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 4072 GCTAAGC GC^T[ACGT]AGC
Cfr10I 3262 GCCGGT [GA]^CCGG[CT]
DraI 2505 TTTAAA TTT^AAA
DraIII 891 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 72 GACGTCCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2864 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 344 AGCGCT AGC^GCT
EcoRV 2214 GATATC GAT^ATC
KasI 899 GGCGCC G^GCGCC
KpnI 3020 GGTACC GGTAC^C
MluI 1820 ACGCGT A^CGCGT
MluNI 2318 TGGCCA TGG^CCA
MscI 2318 TGGCCA TGG^CCA
NarI 899 GGCGCC GG^CGCC
NcoI 2822 CCATGG C^CATGG
NspI 3659 ACATGT [GA]CATG^[CT]
PflFI 2831 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 324 AACGTT AA^CGTT
SacI 852 GAGCTC GAGCT^C
SapI 1196 GAAGAGC GCTCTTC(1/4)
SfoI 899 GGCGCC GGC^GCC
SmaI 383 CCCGGG CCC^GGG
SspBI 3792 TGTACA T^GTACA
Tth111I 2831 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 838 CCAGGGACAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 383 CCCGGG C^CCGGG
XmaI 383 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 72 GACGTC G[GA]^CG[CT]C
899 GGCGCC
AflIII 1820 ACGCGT A^C[GA][CT]GT
3659 ACATGT
ApaI 62 GGGCCC GGGCC^C
106 GGGCCC
BbvCI 3024 CCTCAGC CCTCAGC(-5/-2)
426 GCTGAGG
BsaHI 72 GACGTC G[GA]^CG[CT]C
899 GGCGCC
BsmBI 727 CGTCTC CGTCTC(1/5)
70 GAGACG
BspHI 2381 TCATGA T^CATGA
2911 TCATGA
DsaI 689 CCGTGG C^C[GA][CT]GG
2822 CCATGG
EaeI 557 TGGCCG [CT]^GGCC[GA]
2318 TGGCCA
EcoNI 515 CCTGGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3934 CCTCACCCAGG
Hin1I 72 GACGTC G[GA]^CG[CT]C
899 GGCGCC
HindIII 1724 AAGCTT A^AGCTT
2930 AAGCTT
NdeI 1663 CATATG CA^TATG
3783 CATATG
PflMI 3891 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3965 CCAACACCTGG
PstI 669 CTGCAG CTGCA^G
2856 CTGCAG
RcaI 2381 TCATGA T^CATGA
2911 TCATGA
SexAI 1303 ACCTGGT A^CC[AT]GGT
3970 ACCTGGT
SspI 1489 AATATT AAT^ATT
3770 AATATT
Van91I 3891 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3965 CCAACACCTGG
XbaI 2804 TCTAGA T^CTAGA
3042 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI AocI ApaLI
ApoI Asp700I AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI Eco81I
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI