##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI AocI Aor51HI ApaI
AscI Asp718I AvaI AvrII BanI
BbeI BcgI BciVI BfrI BlnI
BsaBI BsaHI BsiEI BsiWI BsmBI
BsoBI BspDI BspHI BspLU11I BsrBI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT22I FauI
FseI HaeII Hin1I HpaI KasI
KpnI KspI MamI MluI NaeI
NarI NcoI NdeI NgoMIV NotI
NruI NsiI PacI PaeR7I PinAI
PmeI Psp1406I PvuI RcaI RsrII
SacII SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 3020 GTCGAC GT^[AC][GT]AC
AccII 45 CGCG CG^CG
AccIII 67 TCCGGA T^CCGGA
AhdI 1067 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2249 GTGCAC G^TGCAC
ApaLI 2249 GTGCAC G^TGCAC
AspEI 1067 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 74 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3731 TGCGCA TGC^GCA
BalI 3188 TGGCCA TGG^CCA
BbrPI 999 CACGTG CAC^GTG
BbvCI 4244 GCTGAGG CCTCAGC(-5/-2)
BcnI 1117 CCGGG CC^[GC]GG
BglI 55 GCCTCCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 67 TCCGGA [AT]^CCGG[AT]
BseAI 67 TCCGGA T^CCGGA
BsmI 3807 GAATGC GAATGC(1/-1)
Bsp1407I 1379 TGTACA T^GTACA
BspEI 67 TCCGGA T^CCGGA
BspMI 3206 ACCTGC ACCTGC(4/8)
BsrDI 2145 GCAATG GCAATG(2/0)
BsrFI 2734 ACCGGC [GA]^CCGG[CT]
BsrGI 1379 TGTACA T^GTACA
BstBI 450 TTCGAA TT^CGAA
BstUI 45 CGCG CG^CG
CfoI 3732 GCGC GCG^C
Cfr10I 2734 ACCGGC [GA]^CCGG[CT]
DraIII 1542 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 59 CCGTGG C^C[GA][CT]GG
Eam1105I 1067 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 3731 TGCGCA TGC^GCA
HgaI 1478 GCGTC GACGC(5/10)
HhaI 3732 GCGC GCG^C
HinP1I 3732 GCGC G^CGC
HindIII 809 AAGCTT A^AGCTT
MfeI 285 CAATTG C^AATTG
MluNI 3188 TGGCCA TGG^CCA
MroI 67 TCCGGA T^CCGGA
MscI 3188 TGGCCA TGG^CCA
MunI 285 CAATTG C^AATTG
MvnI 45 CGCG CG^CG
NciI 1117 CCGGG CC^[GC]GG
NheI 3814 GCTAGC G^CTAGC
NspV 450 TTCGAA TT^CGAA
PflFI 74 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3567 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 999 CACGTG CAC^GTG
PmlI 999 CACGTG CAC^GTG
PpuMI 3274 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2859 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 3020 GTCGAC G^TCGAC
SfuI 450 TTCGAA TT^CGAA
SmlI 2610 CTTGAG C^T[CT][GA]AG
SspBI 1379 TGTACA T^GTACA
StuI 455 AGGCCT AGG^CCT
Tth111I 74 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3567 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 819 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3256 CAGAAGCTG
BamHI 2899 GGATCC G^GATCC
4256 GGATCC
BanII 225 GAGCTC G[GA]GC[CT]^C
3156 GAGCTC
BclI 772 TGATCA T^GATCA
2750 TGATCA
BglII 1535 AGATCT A^GATCT
3347 AGATCT
BlpI 1137 GCTCAGC GC^T[ACGT]AGC
3225 GCTCAGC
Bpu1102I 1137 GCTCAGC GC^T[ACGT]AGC
3225 GCTCAGC
BsaAI 999 CACGTG [CT]AC^GT[GA]
1864 TACGTG
BsaI 3196 GAGACC GGTCTC(1/5)
2474 GGTCTC
BseRI 215 CTCCTC GAGGAG(10/8)
1080 GAGGAG
BsgI 1983 GTGCAG GTGCAG(16/14)
2006 GTGCAG
BstAPI 308 GCAAATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1881 GCAAAGCATGC
CelII 1137 GCTCAGC GC^T[ACGT]AGC
3225 GCTCAGC
DraI 4134 TTTAAA TTT^AAA
4182 TTTAAA
DraII 3274 AGGTCCT [GA]G^G[ACGT]CC[CT]
4334 AGGCCCT
EaeI 3151 TGGCCG [CT]^GGCC[GA]
3188 TGGCCA
EcoO109I 3274 AGGTCCT [GA]G^G[ACGT]CC[CT]
4334 AGGCCCT
EcoRV 598 GATATC GAT^ATC
2492 GATATC
FbaI 772 TGATCA T^GATCA
2750 TGATCA
MspA1I 822 CAGCTG C[AC]G^C[GT]G
1152 CAGCTG
PvuII 822 CAGCTG CAG^CTG
1152 CAGCTG
SacI 225 GAGCTC GAGCT^C
3156 GAGCTC
SphI 1886 GCATGC GCATG^C
4079 GCATGC
XcmI 2212 CCAAGAAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3191 CCAAGGAGACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI Asp700I AspHI
AvaII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PshBI PstI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII XmnI