##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI AscI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BanI
BbeI BcgI BglI BlnI BlpI
Bpu1102I BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspEI BsrFI
BsrGI BssHII BstAPI BstBI BstEII
BstPI CelII Cfr10I CpoI DrdI
EaeI EagI Eam1105I EarI EclXI
Eco52I EcoO65I EcoRV FseI FspI
Hin1I KasI KpnI Ksp632I KspI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PflFI
PinAI PmeI PshAI PvuI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspBI SwaI Tth111I
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AclI 2020 AACGTT AA^CGTT
Alw44I 2263 GTGCAC G^TGCAC
AlwNI 825 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1259 CCTCAGG CC^T[ACGT]AGG
Aor51HI 49 AGCGCT AGC^GCT
ApaI 1177 GGGCCC GGGCC^C
ApaLI 2263 GTGCAC G^TGCAC
BbsI 525 GAAGAC GAAGAC(2/6)
BclI 1530 TGATCA T^GATCA
BglII 2574 AGATCT A^GATCT
BpuAI 525 GAAGAC GAAGAC(2/6)
BsaBI 328 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 666 GAATGC GAATGC(1/-1)
BspHI 901 TCATGA T^CATGA
BsrDI 647 GCAATG GCAATG(2/0)
BstXI 1128 CCAGAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1259 CCTCAGG CC^T[ACGT]AGG
DraIII 3312 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1059 CCATGG C^C[GA][CT]GG
Eco47III 49 AGCGCT AGC^GCT
Eco81I 1259 CCTCAGG CC^T[ACGT]AGG
EcoNI 767 CCTCCTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 884 ATGCAT ATGCA^T
FbaI 1530 TGATCA T^GATCA
HindIII 3759 AAGCTT A^AGCTT
MamI 328 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 600 CAGCTG C[AC]G^C[GT]G
NcoI 1059 CCATGG C^CATGG
NdeI 3683 CATATG CA^TATG
NsiI 884 ATGCAT ATGCA^T
PflMI 671 CCAGGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 2020 AACGTT AA^CGTT
PvuII 600 CAGCTG CAG^CTG
RcaI 901 TCATGA T^CATGA
SacI 726 GAGCTC GAGCT^C
SpeI 2937 ACTAGT A^CTAGT
SphI 1344 GCATGC GCATG^C
StuI 2758 AGGCCT AGG^CCT
Van91I 671 CCAGGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2513 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AfaI 1323 GTAC GT^AC
2029 GTAC
AflII 2384 CTTAAG C^TTAAG
2815 CTTAAG
AseI 1235 ATTAAT AT^TAAT
3141 ATTAAT
AsnI 1235 ATTAAT AT^TAAT
3141 ATTAAT
AspHI 726 GAGCTC G[AT]GC[AT]^C
2263 GTGCAC
BamHI 142 GGATCC G^GATCC
340 GGATCC
BbrPI 804 CACGTG CAC^GTG
1083 CACGTG
BbvCI 503 CCTCAGC CCTCAGC(-5/-2)
509 CCTCAGC
BciVI 1596 GTATCC GTATCC(6/5)
1724 GTATCC
BcnI 202 CCCGG CC^[GC]GG
611 CCCGG
BfrI 2384 CTTAAG C^TTAAG
2815 CTTAAG
BsaAI 804 CACGTG [CT]AC^GT[GA]
1083 CACGTG
BsgI 2697 CTGCAC GTGCAG(16/14)
707 GTGCAG
BsiHKAI 726 GAGCTC G[AT]GC[AT]^C
2263 GTGCAC
BspDI 149 ATCGAT AT^CGAT
172 ATCGAT
BspLU11I 273 ACATGT A^CATGT
379 ACATGT
BspMI 1247 GCAGGT ACCTGC(4/8)
1537 GCAGGT
BsrBI 2391 CCGCTC CCGCTC(-3/-3)
695 GAGCGG
BssSI 909 CTCGTG CACGAG(-5/-1)
1053 CTCGTG
ClaI 149 ATCGAT AT^CGAT
172 ATCGAT
EcoRI 576 GAATTC G^AATTC
1215 GAATTC
EcoT14I 103 CCTTGG C^C[AT][AT]GG
1059 CCATGG
FauI 31 GCGGG CCCGC(4/6)
697 GCGGG
HaeII 49 AGCGCT [GA]GCGC^[CT]
115 AGCGCC
HapII 203 CCGG C^CGG
612 CCGG
HgaI 1637 GCGTC GACGC(5/10)
1842 GCGTC
HpaII 203 CCGG C^CGG
612 CCGG
HphI 3426 GGTGA GGTGA(8/7)
131 TCACC
MslI 3267 CATACCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3575 CATCCCTATG
MspI 203 CCGG C^CGG
612 CCGG
NciI 202 CCCGG CC^[GC]GG
611 CCCGG
PmaCI 804 CACGTG CAC^GTG
1083 CACGTG
PmlI 804 CACGTG CAC^GTG
1083 CACGTG
PshBI 1235 ATTAAT AT^TAAT
3141 ATTAAT
RsaI 1323 GTAC GT^AC
2029 GTAC
SexAI 2375 ACCAGGT A^CC[AT]GGT
2701 ACCTGGT
SspI 1658 AATATT AAT^ATT
1924 AATATT
StyI 103 CCTTGG C^C[AT][AT]GG
1059 CCATGG
XcmI 846 CCAGAGGTTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1621 CCAGAAATCCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccI AccII AciI AcsI AflIII
AluI AlwI ApoI AvaI AvaII
BanII BbvI BfaI BmyI BpmI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI Bst1107I BstF5I BstNI BstUI
BstYI BstZ17I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HhaI
HinP1I HincII HindII HinfI HpaI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NspI PaeR7I PleI PpuMI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoI
XhoII