##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AgeI        
AhdI        AscI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BanI        
BbeI        BcgI        BglI        BlnI        BlpI        
Bpu1102I    BsaHI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       Bsp1407I    BspEI       BsrFI       
BsrGI       BssHII      BstAPI      BstBI       BstEII      
BstPI       CelII       Cfr10I      CpoI        DrdI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco52I      EcoO65I     EcoRV       FseI        FspI        
Hin1I       KasI        KpnI        Ksp632I     KspI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PflFI       
PinAI       PmeI        PshAI       PvuI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       Sse8387I    SspBI       SwaI        Tth111I     
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AclI         2020  AACGTT               AA^CGTT
Alw44I       2263  GTGCAC               G^TGCAC
AlwNI         825  CAGTCACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1259  CCTCAGG              CC^T[ACGT]AGG
Aor51HI        49  AGCGCT               AGC^GCT
ApaI         1177  GGGCCC               GGGCC^C
ApaLI        2263  GTGCAC               G^TGCAC
BbsI          525  GAAGAC               GAAGAC(2/6)
BclI         1530  TGATCA               T^GATCA
BglII        2574  AGATCT               A^GATCT
BpuAI         525  GAAGAC               GAAGAC(2/6)
BsaBI         328  GATGAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI          666  GAATGC               GAATGC(1/-1)
BspHI         901  TCATGA               T^CATGA
BsrDI         647  GCAATG               GCAATG(2/0)
BstXI        1128  CCAGAGGGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1259  CCTCAGG              CC^T[ACGT]AGG
DraIII       3312  CACATTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1059  CCATGG               C^C[GA][CT]GG
Eco47III       49  AGCGCT               AGC^GCT
Eco81I       1259  CCTCAGG              CC^T[ACGT]AGG
EcoNI         767  CCTCCTCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       884  ATGCAT               ATGCA^T
FbaI         1530  TGATCA               T^GATCA
HindIII      3759  AAGCTT               A^AGCTT
MamI          328  GATGAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I        600  CAGCTG               C[AC]G^C[GT]G
NcoI         1059  CCATGG               C^CATGG
NdeI         3683  CATATG               CA^TATG
NsiI          884  ATGCAT               ATGCA^T
PflMI         671  CCAGGGGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I     2020  AACGTT               AA^CGTT
PvuII         600  CAGCTG               CAG^CTG
RcaI          901  TCATGA               T^CATGA
SacI          726  GAGCTC               GAGCT^C
SpeI         2937  ACTAGT               A^CTAGT
SphI         1344  GCATGC               GCATG^C
StuI         2758  AGGCCT               AGG^CCT
Van91I        671  CCAGGGGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         2513  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

AfaI         1323  GTAC                 GT^AC
             2029  GTAC                
AflII        2384  CTTAAG               C^TTAAG
             2815  CTTAAG              
AseI         1235  ATTAAT               AT^TAAT
             3141  ATTAAT              
AsnI         1235  ATTAAT               AT^TAAT
             3141  ATTAAT              
AspHI         726  GAGCTC               G[AT]GC[AT]^C
             2263  GTGCAC              
BamHI         142  GGATCC               G^GATCC
              340  GGATCC              
BbrPI         804  CACGTG               CAC^GTG
             1083  CACGTG              
BbvCI         503  CCTCAGC              CCTCAGC(-5/-2)
              509  CCTCAGC             
BciVI        1596  GTATCC               GTATCC(6/5)
             1724  GTATCC              
BcnI          202  CCCGG                CC^[GC]GG
              611  CCCGG               
BfrI         2384  CTTAAG               C^TTAAG
             2815  CTTAAG              
BsaAI         804  CACGTG               [CT]AC^GT[GA]
             1083  CACGTG              
BsgI         2697  CTGCAC               GTGCAG(16/14)
              707  GTGCAG              
BsiHKAI       726  GAGCTC               G[AT]GC[AT]^C
             2263  GTGCAC              
BspDI         149  ATCGAT               AT^CGAT
              172  ATCGAT              
BspLU11I      273  ACATGT               A^CATGT
              379  ACATGT              
BspMI        1247  GCAGGT               ACCTGC(4/8)
             1537  GCAGGT              
BsrBI        2391  CCGCTC               CCGCTC(-3/-3)
              695  GAGCGG              
BssSI         909  CTCGTG               CACGAG(-5/-1)
             1053  CTCGTG              
ClaI          149  ATCGAT               AT^CGAT
              172  ATCGAT              
EcoRI         576  GAATTC               G^AATTC
             1215  GAATTC              
EcoT14I       103  CCTTGG               C^C[AT][AT]GG
             1059  CCATGG              
FauI           31  GCGGG                CCCGC(4/6)
              697  GCGGG               
HaeII          49  AGCGCT               [GA]GCGC^[CT]
              115  AGCGCC              
HapII         203  CCGG                 C^CGG
              612  CCGG                
HgaI         1637  GCGTC                GACGC(5/10)
             1842  GCGTC               
HpaII         203  CCGG                 C^CGG
              612  CCGG                
HphI         3426  GGTGA                GGTGA(8/7)
              131  TCACC               
MslI         3267  CATACCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             3575  CATCCCTATG          
MspI          203  CCGG                 C^CGG
              612  CCGG                
NciI          202  CCCGG                CC^[GC]GG
              611  CCCGG               
PmaCI         804  CACGTG               CAC^GTG
             1083  CACGTG              
PmlI          804  CACGTG               CAC^GTG
             1083  CACGTG              
PshBI        1235  ATTAAT               AT^TAAT
             3141  ATTAAT              
RsaI         1323  GTAC                 GT^AC
             2029  GTAC                
SexAI        2375  ACCAGGT              A^CC[AT]GGT
             2701  ACCTGGT             
SspI         1658  AATATT               AAT^ATT
             1924  AATATT              
StyI          103  CCTTGG               C^C[AT][AT]GG
             1059  CCATGG              
XcmI          846  CCAGAGGTTATCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1621  CCAGAAATCCACTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AflIII      
AluI        AlwI        ApoI        AvaI        AvaII       
BanII       BbvI        BfaI        BmyI        BpmI        
BsaI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrI        
BssKI       Bst1107I    BstF5I      BstNI       BstUI       
BstYI       BstZ17I     Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HincII      HindII      HinfI       HpaI        
ItaI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MvaI        
MvnI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PaeR7I      PleI        PpuMI       PstI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoI        
XhoII