##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp718I AspEI
AvrII BbrPI BcgI BclI BfrI
BglII BlnI BsaAI BsaBI BsiWI
Bsp1407I BspDI BspLU11I BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Cfr10I ClaI CpoI DraIII
DrdI Eam1105I EcoO65I EcoRV FbaI
FseI HincII HindII HindIII HpaI
KpnI MamI MfeI MluI MunI
NaeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SalI SbfI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SwaI
XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 805 TCCGGA T^CCGGA
Asp700I 3743 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1594 GGATCC G^GATCC
BciVI 2492 GGATAC GTATCC(6/5)
BlpI 2130 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2130 GCTCAGC GC^T[ACGT]AGC
BseAI 805 TCCGGA T^CCGGA
BsiEI 2810 CGGCCG CG[GA][CT]^CG
BsmI 1094 GCATTC GAATGC(1/-1)
BspEI 805 TCCGGA T^CCGGA
BspHI 1480 TCATGA T^CATGA
CelII 2130 GCTCAGC GC^T[ACGT]AGC
DraI 4136 TTTAAA TTT^AAA
EagI 2810 CGGCCG C^GGCCG
EclXI 2810 CGGCCG C^GGCCG
Eco52I 2810 CGGCCG C^GGCCG
EcoNI 733 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4144 GAATTC G^AATTC
KspI 2517 CCGCGG CCGC^GG
MaeII 984 ACGT A^CGT
MroI 805 TCCGGA T^CCGGA
NdeI 3 CATATG CA^TATG
NspI 1503 ACATGC [GA]CATG^[CT]
PshAI 3265 GACTAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1480 TCATGA T^CATGA
SacII 2517 CCGCGG CCGC^GG
ScaI 2070 AGTACT AGT^ACT
SspI 1946 AATATT AAT^ATT
XmnI 3743 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 2147 CGCG CG^CG
2518 CGCG
AcyI 742 GGCGCC G[GA]^CG[CT]C
2889 GGCGCC
Aor51HI 1032 AGCGCT AGC^GCT
1116 AGCGCT
AviII 1266 TGCGCA TGC^GCA
3057 TGCGCA
BbeI 742 GGCGCC GGCGC^C
2889 GGCGCC
BbsI 131 GAAGAC GAAGAC(2/6)
1496 GTCTTC
BglI 545 GCCCGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2121 GCCCAACTGGC
BpuAI 131 GAAGAC GAAGAC(2/6)
1496 GTCTTC
BsaHI 742 GGCGCC G[GA]^CG[CT]C
2889 GGCGCC
BsaI 3030 GAGACC GGTCTC(1/5)
3898 GGTCTC
BsmBI 668 GAGACG CGTCTC(1/5)
3868 GAGACG
BsrBI 488 CCGCTC CCGCTC(-3/-3)
2098 CCGCTC
BsrDI 1692 CATTGC GCAATG(2/0)
3577 GCAATG
BssSI 9 CACGAG CACGAG(-5/-1)
2041 CACGAG
BstAPI 1875 GCACACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2424 GCATGGTGTGC
BstUI 2147 CGCG CG^CG
2518 CGCG
Eco47III 1032 AGCGCT AGC^GCT
1116 AGCGCT
EcoT22I 1528 ATGCAT ATGCA^T
2368 ATGCAT
FspI 1266 TGCGCA TGC^GCA
3057 TGCGCA
HgaI 670 GACGC GACGC(5/10)
609 GCGTC
Hin1I 742 GGCGCC G[GA]^CG[CT]C
2889 GGCGCC
KasI 742 GGCGCC G^GCGCC
2889 GGCGCC
MvnI 2147 CGCG CG^CG
2518 CGCG
NarI 742 GGCGCC GG^CGCC
2889 GGCGCC
NsiI 1528 ATGCAT ATGCA^T
2368 ATGCAT
SapI 1023 GAAGAGC GCTCTTC(1/4)
2859 GAAGAGC
SfoI 742 GGCGCC GGC^GCC
2889 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AspI AvaI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BmyI BpmI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflFI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XhoII XmaCI XmaI