##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AflII AgeI AhdI
AocI Aor51HI AscI Asp718I AspEI
AspI AviII BbeI BclI BfrI
BglII BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII Bst1107I BstZ17I
Bsu36I ClaI Eam1105I Eco47III Eco81I
EcoNI EcoRV FbaI FseI FspI
HindIII HpaI KasI KpnI KspI
MfeI MluI MunI NarI NdeI
NheI NotI PacI PaeR7I PflFI
PinAI PmeI PshAI PvuI RcaI
SacII SalI ScaI SexAI SfoI
SgrAI SmaI SnaBI SpeI SspBI
SspI Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 1280 TCCGGA T^CCGGA
AclI 4199 AACGTT AA^CGTT
AcyI 2057 GGCGTC G[GA]^CG[CT]C
AflIII 1500 ACGTGT A^C[GA][CT]GT
AseI 4415 ATTAAT AT^TAAT
AsnI 4415 ATTAAT AT^TAAT
AvrII 3929 CCTAGG C^CTAGG
BamHI 3536 GGATCC G^GATCC
BbrPI 1499 CACGTG CAC^GTG
BbvCI 3034 CCTCAGC CCTCAGC(-5/-2)
BcgI 309 GCAAGTCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 3929 CCTAGG C^CTAGG
BlpI 545 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 545 GCTGAGC GC^T[ACGT]AGC
BsaAI 1499 CACGTG [CT]AC^GT[GA]
BsaBI 2171 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2057 GGCGTC G[GA]^CG[CT]C
BseAI 1280 TCCGGA T^CCGGA
BsmBI 4046 CGTCTC CGTCTC(1/5)
BspEI 1280 TCCGGA T^CCGGA
BsrBI 2850 CCGCTC CCGCTC(-3/-3)
BsrDI 1101 GCAATG GCAATG(2/0)
BsrFI 571 GCCGGC [GA]^CCGG[CT]
BstBI 1044 TTCGAA TT^CGAA
BstEII 2818 GGTGACC G^GT[ACGT]ACC
BstPI 2818 GGTGACC G^GT[ACGT]ACC
CelII 545 GCTGAGC GC^T[ACGT]AGC
Cfr10I 571 GCCGGC [GA]^CCGG[CT]
CpoI 2658 CGGTCCG CG^G[AT]CCG
DrdI 1784 GACAACGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2818 GGTGACC G^GT[ACGT]ACC
EcoT22I 3946 ATGCAT ATGCA^T
Hin1I 2057 GGCGTC G[GA]^CG[CT]C
HincII 444 GTCAAC GT[CT]^[GA]AC
HindII 444 GTCAAC GT[CT]^[GA]AC
MamI 2171 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1280 TCCGGA T^CCGGA
NaeI 571 GCCGGC GCC^GGC
NgoMIV 571 GCCGGC G^CCGGC
NruI 83 TCGCGA TCG^CGA
NsiI 3946 ATGCAT ATGCA^T
NspV 1044 TTCGAA TT^CGAA
PmaCI 1499 CACGTG CAC^GTG
PmlI 1499 CACGTG CAC^GTG
PshBI 4415 ATTAAT AT^TAAT
Psp1406I 4199 AACGTT AA^CGTT
RsrII 2658 CGGTCCG CG^G[AT]CCG
SfiI 1981 GGCCCGCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1044 TTCGAA TT^CGAA
SwaI 4241 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 3120 GTAGAC GT^[AC][GT]AC
3235 GTAGAC
Alw44I 779 GTGCAC G^TGCAC
1203 GTGCAC
ApaI 3443 GGGCCC GGGCC^C
3698 GGGCCC
ApaLI 779 GTGCAC G^TGCAC
1203 GTGCAC
Asp700I 256 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1336 GAAGCTCTTC
BalI 1038 TGGCCA TGG^CCA
3400 TGGCCA
BsaWI 901 ACCGGA [AT]^CCGG[AT]
1280 TCCGGA
BsmI 1642 GAATGC GAATGC(1/-1)
1415 GCATTC
BssSI 1703 CACGAG CACGAG(-5/-1)
2861 CACGAG
BstXI 1542 CCAGTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2574 CCAAGATCCTGG
DraI 3489 TTTAAA TTT^AAA
4242 TTTAAA
EcoRI 1013 GAATTC G^AATTC
3138 GAATTC
HaeII 1155 GGCGCT [GA]GCGC^[CT]
4056 AGCGCC
MluNI 1038 TGGCCA TGG^CCA
3400 TGGCCA
MscI 1038 TGGCCA TGG^CCA
3400 TGGCCA
SacI 1367 GAGCTC GAGCT^C
2627 GAGCTC
SbfI 835 CCTGCAGG CCTGCA^GG
2824 CCTGCAGG
Sse8387I 835 CCTGCAGG CCTGCA^GG
2824 CCTGCAGG
StuI 1452 AGGCCT AGG^CCT
1989 AGGCCT
XmnI 256 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1336 GAAGCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BciVI BcnI BfaI BglI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII