##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BalI BanI BanII BbeI BbrPI
BglII BlnI BsaAI BsaHI BsaWI
BseAI BsiEI BsiWI BspEI BspLU11I
BsrBI BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CfoI CpoI DraIII EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
KasI KpnI KspI MaeII MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NspV PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI PvuII RsrII
SacI SacII SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 1921 GTCGAC GT^[AC][GT]AC
AseI 3721 ATTAAT AT^TAAT
AsnI 3721 ATTAAT AT^TAAT
AspHI 2101 GTGCTC G[AT]GC[AT]^C
BamHI 1762 GGATCC G^GATCC
BcgI 747 GCATCAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1475 GGATAC GTATCC(6/5)
BglI 2119 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 353 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 3696 CTGCAC GTGCAG(16/14)
BsiHKAI 2101 GTGCTC G[AT]GC[AT]^C
BsmBI 1139 CGTCTC CGTCTC(1/5)
BsmI 2172 GAATGC GAATGC(1/-1)
Bsp1407I 1396 TGTACA T^GTACA
BspDI 4270 ATCGAT AT^CGAT
BsrFI 494 ACCGGC [GA]^CCGG[CT]
BsrGI 1396 TGTACA T^GTACA
BstAPI 498 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2522 CCATTATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 494 ACCGGC [GA]^CCGG[CT]
ClaI 4270 ATCGAT AT^CGAT
DraII 655 AGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 1131 GACCTGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 655 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRV 242 GATATC GAT^ATC
EcoT22I 2604 ATGCAT ATGCA^T
FauI 2289 GCGGG CCCGC(4/6)
MamI 353 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 2604 ATGCAT ATGCA^T
PacI 3400 TTAATTAA TTAAT^TAA
PflMI 3566 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1152 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3721 ATTAAT AT^TAAT
SalI 1921 GTCGAC G^TCGAC
SapI 1176 GAAGAGC GCTCTTC(1/4)
SpeI 2990 ACTAGT A^CTAGT
SspBI 1396 TGTACA T^GTACA
SspI 2366 AATATT AAT^ATT
Van91I 3566 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1255 CCAAAGAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 1814 CCGC CCGC(-3/-1)
2289 GCGG
Asp700I 2364 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2594 GAATGTTTTC
AvaI 376 CCCGAG C^[CT]CG[GA]G
697 CCCGAG
BclI 302 TGATCA T^GATCA
1983 TGATCA
BcnI 5 CCCGG CC^[GC]GG
3542 CCCGG
BlpI 1607 GCTGAGC GC^T[ACGT]AGC
1721 GCTCAGC
Bpu1102I 1607 GCTGAGC GC^T[ACGT]AGC
1721 GCTCAGC
BsaI 936 GAGACC GGTCTC(1/5)
1730 GAGACC
BsoBI 376 CCCGAG C^[CT]CG[GA]G
697 CCCGAG
BspHI 1910 TCATGA T^CATGA
2897 TCATGA
CelII 1607 GCTGAGC GC^T[ACGT]AGC
1721 GCTCAGC
EcoNI 570 CCTCCGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1831 CCTGGGCCAGG
FbaI 302 TGATCA T^GATCA
1983 TGATCA
NciI 5 CCCGG CC^[GC]GG
3542 CCCGG
NcoI 1012 CCATGG C^CATGG
3296 CCATGG
NdeI 3337 CATATG CA^TATG
3345 CATATG
RcaI 1910 TCATGA T^CATGA
2897 TCATGA
XmnI 2364 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2594 GAATGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AcsI AfaI AflII AluI AlwI
AlwNI ApoI AvaII BbsI BbvCI
BbvI BfaI BfrI BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DsaI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HincII HindII HindIII
HinfI HpaI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII