##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AgeI        
Aor51HI     AscI        AseI        AsnI        AviII       
BamHI       BbeI        BciVI       BsaBI       BseAI       
BsiWI       Bsp1407I    BspDI       BspEI       BspHI       
BsrGI       BssHII      BstBI       BstUI       ClaI        
CpoI        DraI        DraIII      Eco47III    EcoRV       
EcoT22I     FseI        FspI        HindIII     HpaI        
KasI        KspI        MamI        MfeI        MluI        
MroI        MunI        MvnI        NarI        NdeI        
NotI        NruI        NsiI        NspV        PacI        
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        SfoI        SfuI        SgrAI       SmaI        
SphI        Sse8387I    SspBI       SwaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I        537  GGTACC               G^GTACC
AflII        1185  CTTAAG               C^TTAAG
AflIII       1873  ACATGT               A^C[GA][CT]GT
AhdI         1615  GACGCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1755  GTGCAC               G^TGCAC
ApaLI        1755  GTGCAC               G^TGCAC
Asp718I       537  GGTACC               G^GTACC
AspEI        1615  GACGCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1909  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          474  CTCGAG               C^[CT]CG[GA]G
AvrII        3635  CCTAGG               C^CTAGG
BalI         1291  TGGCCA               TGG^CCA
BbrPI        3514  CACGTG               CAC^GTG
BcgI           20  GCAGTAGGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          928  TGATCA               T^GATCA
BfrI         1185  CTTAAG               C^TTAAG
BglII        1066  AGATCT               A^GATCT
BlnI         3635  CCTAGG               C^CTAGG
BsaWI        2567  TCCGGT               [AT]^CCGG[AT]
BsiEI        2672  CGGCCG               CG[GA][CT]^CG
BsmBI        3915  CGTCTC               CGTCTC(1/5)
BsoBI         474  CTCGAG               C^[CT]CG[GA]G
BspLU11I     1873  ACATGT               A^CATGT
BstEII        945  GGTTACC              G^GT[ACGT]ACC
BstPI         945  GGTTACC              G^GT[ACGT]ACC
EagI         2672  CGGCCG               C^GGCCG
Eam1105I     1615  GACGCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2672  CGGCCG               C^GGCCG
Eco52I       2672  CGGCCG               C^GGCCG
EcoO65I       945  GGTTACC              G^GT[ACGT]ACC
FbaI          928  TGATCA               T^GATCA
KpnI          537  GGTACC               GGTAC^C
MluNI        1291  TGGCCA               TGG^CCA
MscI         1291  TGGCCA               TGG^CCA
NheI          254  GCTAGC               G^CTAGC
PaeR7I        474  CTCGAG               C^TCGAG
PflFI        1909  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        3514  CACGTG               CAC^GTG
PmlI         3514  CACGTG               CAC^GTG
ScaI          439  AGTACT               AGT^ACT
SexAI        3082  ACCAGGT              A^CC[AT]GGT
SfiI         2108  GGCCCAACAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI        4386  TACGTA               TAC^GTA
SpeI         1704  ACTAGT               A^CTAGT
SspI          570  AATATT               AAT^ATT
Tth111I      1909  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         3171  TCTAGA               T^CTAGA
XcmI          334  CCATTCAACAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          474  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AcyI         1615  GACGCC               G[GA]^CG[CT]C
             1909  GACGCC              
AocI         2001  CCTGAGG              CC^T[ACGT]AGG
             2952  CCTGAGG             
ApaI         2473  GGGCCC               GGGCC^C
             4309  GGGCCC              
BsaAI        3514  CACGTG               [CT]AC^GT[GA]
             4386  TACGTA              
BsaHI        1615  GACGCC               G[GA]^CG[CT]C
             1909  GACGCC              
BsmI         3560  GAATGC               GAATGC(1/-1)
             3477  GCATTC              
BsrBI         477  GAGCGG               CCGCTC(-3/-3)
             1842  GAGCGG              
BssSI         289  CACGAG               CACGAG(-5/-1)
             1805  CACGAG              
Bst1107I     3132  GTATAC               GTA^TAC
             4346  GTATAC              
BstZ17I      3132  GTATAC               GTA^TAC
             4346  GTATAC              
Bsu36I       2001  CCTGAGG              CC^T[ACGT]AGG
             2952  CCTGAGG             
Eco81I       2001  CCTGAGG              CC^T[ACGT]AGG
             2952  CCTGAGG             
EcoRI         886  GAATTC               G^AATTC
             2091  GAATTC              
HaeII        1821  AGCGCC               [GA]GCGC^[CT]
             2860  GGCGCT              
Hin1I        1615  GACGCC               G[GA]^CG[CT]C
             1909  GACGCC              
NaeI          577  GCCGGC               GCC^GGC
              954  GCCGGC              
NgoMIV        577  GCCGGC               G^CCGGC
              954  GCCGGC              
PpuMI         104  GGGACCT              [GA]G^G[AT]CC[CT]
             2377  GGGTCCC             
StuI         2026  AGGCCT               AGG^CCT
             3904  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  119 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaII       BanI        BanII       BbsI        BbvCI       
BbvI        BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BsrDI       BsrFI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DrdI        DsaI        EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PstI        PvuII       RsaI        SacI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoII       XmnI