##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Aor51HI     
ApaI        AscI        Asp718I     AspEI       AviII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BfrI        BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BspDI       BspEI       BsrBI       
BssHII      BssSI       Bst1107I    BstBI       BstUI       
BstXI       BstZ17I     ClaI        CpoI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoT22I     FauI        FseI        FspI        
Hin1I       KasI        KpnI        KspI        MfeI        
MluI        MroI        MunI        MvnI        NarI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SpeI        SphI        Sse8387I    StuI        
Van91I      XhoI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Alw44I       4209  GTGCAC               G^TGCAC
AocI         2904  CCTCAGG              CC^T[ACGT]AGG
ApaLI        4209  GTGCAC               G^TGCAC
AspI         2155  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         3096  CCCGGG               C^[CT]CG[GA]G
AvrII        1833  CCTAGG               C^CTAGG
BalI         3117  TGGCCA               TGG^CCA
BciVI        1026  GTATCC               GTATCC(6/5)
BclI         1041  TGATCA               T^GATCA
BglI         2869  GCCATTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         1833  CCTAGG               C^CTAGG
BlpI         2330  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2330  GCTCAGC              GC^T[ACGT]AGC
BsaAI        2671  TACGTA               [CT]AC^GT[GA]
BsaI         1687  GGTCTC               GGTCTC(1/5)
BsoBI        3096  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     1081  TGTACA               T^GTACA
BsrFI        3734  GCCGGC               [GA]^CCGG[CT]
BsrGI        1081  TGTACA               T^GTACA
BstAPI       4202  GCACATTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       3724  GGTCACC              G^GT[ACGT]ACC
BstPI        3724  GGTCACC              G^GT[ACGT]ACC
Bsu36I       2904  CCTCAGG              CC^T[ACGT]AGG
CelII        2330  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       3734  GCCGGC               [GA]^CCGG[CT]
DraIII       4203  CACATTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         3655  CCATGG               C^C[GA][CT]GG
EaeI         3117  TGGCCA               [CT]^GGCC[GA]
Eco81I       2904  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      3724  GGTCACC              G^GT[ACGT]ACC
EcoRI        3715  GAATTC               G^AATTC
FbaI         1041  TGATCA               T^GATCA
HaeII        3165  AGCGCC               [GA]GCGC^[CT]
HpaI          271  GTTAAC               GTT^AAC
MluNI        3117  TGGCCA               TGG^CCA
MscI         3117  TGGCCA               TGG^CCA
MspA1I       2333  CAGCTG               C[AC]G^C[GT]G
NaeI         3734  GCCGGC               GCC^GGC
NcoI         3655  CCATGG               C^CATGG
NdeI         2165  CATATG               CA^TATG
NgoMIV       3734  GCCGGC               G^CCGGC
PflFI        2155  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuII        2333  CAGCTG               CAG^CTG
SmaI         3096  CCCGGG               CCC^GGG
SnaBI        2671  TACGTA               TAC^GTA
SspBI        1081  TGTACA               T^GTACA
SwaI         1556  ATTTAAAT             ATTT^AAAT
TaqI         1353  TCGA                 T^CGA
Tth111I      2155  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      1353  TCGA                 T^CGA
XbaI         2760  TCTAGA               T^CTAGA
XmaCI        3096  CCCGGG               C^CCGGG
XmaI         3096  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AlwNI         593  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             3164  CAGCGCCTG           
BcnI         3096  CCCGG                CC^[GC]GG
             3097  CCGGG               
BsaBI         624  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1810  GATTACCATC          
BseRI        1382  CTCCTC               GAGGAG(10/8)
             2984  CTCCTC              
BsmBI        3105  CGTCTC               CGTCTC(1/5)
             3176  GAGACG              
BsmI         2865  GAATGC               GAATGC(1/-1)
             2899  GCATTC              
BspHI        3221  TCATGA               T^CATGA
             3582  TCATGA              
BspMI        4198  ACCTGC               ACCTGC(4/8)
             3196  GCAGGT              
BsrDI        1107  GCAATG               GCAATG(2/0)
             2269  GCAATG              
DraII        2038  GGGACCT              [GA]G^G[ACGT]CC[CT]
             3510  GGGTCCT             
EcoO109I     2038  GGGACCT              [GA]G^G[ACGT]CC[CT]
             3510  GGGTCCT             
EcoRV        1206  GATATC               GAT^ATC
             1657  GATATC              
HaeIII       3118  GGCC                 GG^CC
             3494  GGCC                
HapII        3097  CCGG                 C^CGG
             3735  CCGG                
HgaI         3178  GACGC                GACGC(5/10)
             2559  GCGTC               
HincII        224  GTCAAC               GT[CT]^[GA]AC
              271  GTTAAC              
HindII        224  GTCAAC               GT[CT]^[GA]AC
              271  GTTAAC              
HindIII       741  AAGCTT               A^AGCTT
             1288  AAGCTT              
HpaII        3097  CCGG                 C^CGG
             3735  CCGG                
MamI          624  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1810  GATTACCATC          
MspI         3097  CCGG                 C^CGG
             3735  CCGG                
NciI         3096  CCCGG                CC^[GC]GG
             3097  CCGGG               
PpuMI        2038  GGGACCT              [GA]G^G[AT]CC[CT]
             3510  GGGTCCT             
PstI         1625  CTGCAG               CTGCA^G
             3086  CTGCAG              
RcaI         3221  TCATGA               T^CATGA
             3582  TCATGA              
SmlI         2955  CTTGAG               C^T[CT][GA]AG
             4085  CTCAAG              
SspI          517  AATATT               AAT^ATT
             1359  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        AlwI        ApoI        AseI        AsnI        
Asp700I     AspHI       AvaII       BanII       BbsI        
BbvI        BfaI        BglII       BmyI        BpmI        
BpuAI       BsaJI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspLU11I    
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        EarI        EcoRII      EcoT14I     Fnu4HI      
FokI        HhaI        HinP1I      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PshBI       RsaI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XcmI        XhoII       
XmnI