##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AhdI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
BamHI       BbeI        BbrPI       BclI        BfrI        
BsaAI       BsaBI       BseAI       BsiEI       BsiWI       
Bsp1407I    BspEI       BsrGI       BssHII      BssSI       
Bst1107I    BstEII      BstPI       BstZ17I     CpoI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       EcoRV       FbaI        FseI        
HaeII       HindIII     HpaI        KasI        KpnI        
KspI        MaeII       MamI        MfeI        MluI        
MroI        MunI        NarI        NdeI        NheI        
NotI        NruI        PacI        PflMI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
ScaI        SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       StuI        
SwaI        Van91I      XbaI        XcmI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccII         958  CGCG                 CG^CG
AcyI         3638  GACGCC               G[GA]^CG[CT]C
AflIII       4175  ACATGT               A^C[GA][CT]GT
AgeI         3439  ACCGGT               A^CCGGT
ApaI         3160  GGGCCC               GGGCC^C
Asp700I      2903  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1257  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        3189  CCTAGG               C^CTAGG
BciVI        3199  GTATCC               GTATCC(6/5)
BglII        2085  AGATCT               A^GATCT
BlnI         3189  CCTAGG               C^CTAGG
BsaHI        3638  GACGCC               G[GA]^CG[CT]C
BsmBI         974  CGTCTC               CGTCTC(1/5)
BspDI         293  ATCGAT               AT^CGAT
BspLU11I     4175  ACATGT               A^CATGT
BstBI        1601  TTCGAA               TT^CGAA
BstUI         958  CGCG                 CG^CG
ClaI          293  ATCGAT               AT^CGAT
DraI         3559  TTTAAA               TTT^AAA
DraIII       3790  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         2388  GACTCGAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I       287  ATGCAT               ATGCA^T
HgaI         3638  GACGC                GACGC(5/10)
Hin1I        3638  GACGCC               G[GA]^CG[CT]C
MvnI          958  CGCG                 CG^CG
NaeI         1284  GCCGGC               GCC^GGC
NgoMIV       1284  GCCGGC               G^CCGGC
NsiI          287  ATGCAT               ATGCA^T
NspV         1601  TTCGAA               TT^CGAA
PaeR7I       2390  CTCGAG               C^TCGAG
PflFI        1257  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        3439  ACCGGT               A^CCGGT
SacI         2131  GAGCTC               GAGCT^C
SfuI         1601  TTCGAA               TT^CGAA
SphI         1941  GCATGC               GCATG^C
SspI         3824  AATATT               AAT^ATT
Tth111I      1257  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         2390  CTCGAG               C^TCGAG
XmnI         2903  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AvaI         2390  CTCGAG               C^[CT]CG[GA]G
             3349  CCCGAG              
AviII        1992  TGCGCA               TGC^GCA
             2527  TGCGCA              
BalI          141  TGGCCA               TGG^CCA
             3848  TGGCCA              
BlpI         2440  GCTGAGC              GC^T[ACGT]AGC
             3250  GCTAAGC             
Bpu1102I     2440  GCTGAGC              GC^T[ACGT]AGC
             3250  GCTAAGC             
BsaI         1998  GGTCTC               GGTCTC(1/5)
             4098  GGTCTC              
BsaWI        2186  ACCGGA               [AT]^CCGG[AT]
             3439  ACCGGT              
BsgI         2113  GTGCAG               GTGCAG(16/14)
             4143  GTGCAG              
BsmI          776  GAATGC               GAATGC(1/-1)
             3811  GCATTC              
BsoBI        2390  CTCGAG               C^[CT]CG[GA]G
             3349  CCCGAG              
BsrDI         549  CATTGC               GCAATG(2/0)
               43  GCAATG              
BstAPI        280  GCAGTCCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1351  GCACCTTCTGC         
BstYI        1784  AGATCC               [GA]^GATC[CT]
             2085  AGATCT              
CelII        2440  GCTGAGC              GC^T[ACGT]AGC
             3250  GCTAAGC             
CfoI         1993  GCGC                 GCG^C
             2528  GCGC                
EcoNI         184  CCTAAGAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2217  CCTGGAGGAGG         
FspI         1992  TGCGCA               TGC^GCA
             2527  TGCGCA              
HhaI         1993  GCGC                 GCG^C
             2528  GCGC                
HinP1I       1993  GCGC                 G^CGC
             2528  GCGC                
HincII        392  GTCAAC               GT[CT]^[GA]AC
             4148  GTCAAC              
HindII        392  GTCAAC               GT[CT]^[GA]AC
             4148  GTCAAC              
MflI         1784  AGATCC               [GA]^GATC[CT]
             2085  AGATCT              
MluNI         141  TGGCCA               TGG^CCA
             3848  TGGCCA              
MscI          141  TGGCCA               TGG^CCA
             3848  TGGCCA              
PpuMI         596  AGGACCT              [GA]G^G[AT]CC[CT]
             3359  GGGACCC             
SapI         2890  GAAGAGC              GCTCTTC(1/4)
             1060  GCTCTTC             
SexAI        2720  ACCAGGT              A^CC[AT]GGT
             3878  ACCTGGT             
XhoII        1784  AGATCC               [GA]^GATC[CT]
             2085  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApoI        AspHI       AvaII       
BanI        BanII       BbsI        BbvCI       BbvI        
BcgI        BcnI        BfaI        BglI        BmyI        
BpmI        BpuAI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspHI       BspMI       BsrBI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstXI       Bsu36I      
Cac8I       Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco81I      
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PstI        PvuII       RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I