##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII Acc65I AflII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI AviII BciVI BclI
BfrI BsaBI BsiWI BspDI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI Eam1105I EcoO65I EcoRI EcoRV
FbaI FseI FspI HpaI KpnI
MamI MluI NcoI NdeI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshBI PvuI RsrII SapI
SbfI SfiI SfuI SgrAI Sse8387I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 2231 TCCGGA T^CCGGA
AclI 4198 AACGTT AA^CGTT
Aor51HI 491 AGCGCT AGC^GCT
AspI 3238 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2802 CCTAGG C^CTAGG
BamHI 1340 GGATCC G^GATCC
BbrPI 1684 CACGTG CAC^GTG
BglII 2616 AGATCT A^GATCT
BlnI 2802 CCTAGG C^CTAGG
BlpI 2170 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2170 GCTCAGC GC^T[ACGT]AGC
BsaI 2502 GAGACC GGTCTC(1/5)
BsaWI 2231 TCCGGA [AT]^CCGG[AT]
BseAI 2231 TCCGGA T^CCGGA
BsmI 1392 GAATGC GAATGC(1/-1)
BspEI 2231 TCCGGA T^CCGGA
BspHI 3746 TCATGA T^CATGA
BspLU11I 4173 ACATGT A^CATGT
BssSI 2877 CACGAG CACGAG(-5/-1)
BstAPI 4103 GCAGGTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 2170 GCTCAGC GC^T[ACGT]AGC
DraIII 3399 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 491 AGCGCT AGC^GCT
EcoT22I 3843 ATGCAT ATGCA^T
HincII 2661 GTCGAC GT[CT]^[GA]AC
HindII 2661 GTCGAC GT[CT]^[GA]AC
HindIII 1375 AAGCTT A^AGCTT
KspI 2003 CCGCGG CCGC^GG
MroI 2231 TCCGGA T^CCGGA
MslI 1842 CATGGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1766 GCCGGC GCC^GGC
NgoMIV 1766 GCCGGC G^CCGGC
NheI 278 GCTAGC G^CTAGC
NsiI 3843 ATGCAT ATGCA^T
PflFI 3238 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1684 CACGTG CAC^GTG
PmlI 1684 CACGTG CAC^GTG
PshAI 2212 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4198 AACGTT AA^CGTT
RcaI 3746 TCATGA T^CATGA
SacII 2003 CCGCGG CCGC^GG
SalI 2661 GTCGAC G^TCGAC
ScaI 1244 AGTACT AGT^ACT
SmlI 1138 CTTGAG C^T[CT][GA]AG
SnaBI 3543 TACGTA TAC^GTA
SpeI 3567 ACTAGT A^CTAGT
StuI 1034 AGGCCT AGG^CCT
SwaI 3997 ATTTAAAT ATTT^AAAT
Tth111I 3238 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 2610 GTCTAC GT^[AC][GT]AC
2661 GTCGAC
AflIII 1683 ACACGT A^C[GA][CT]GT
4173 ACATGT
Asp700I 306 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
379 GAAGACCTTC
BbsI 379 GAAGAC GAAGAC(2/6)
783 GTCTTC
BbvCI 1773 CCTCAGC CCTCAGC(-5/-2)
2521 CCTCAGC
BcgI 1950 CGAGCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
309 GCAGTTCAATCG
BpuAI 379 GAAGAC GAAGAC(2/6)
783 GTCTTC
BsaAI 1684 CACGTG [CT]AC^GT[GA]
3543 TACGTA
BspMI 2937 GCAGGT ACCTGC(4/8)
4103 GCAGGT
BsrBI 3731 CCGCTC CCGCTC(-3/-3)
25 GAGCGG
BsrDI 55 CATTGC GCAATG(2/0)
72 CATTGC
BssHII 81 GCGCGC G^CGCGC
154 GCGCGC
BstYI 1340 GGATCC [GA]^GATC[CT]
2616 AGATCT
DrdI 774 GACTTCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1569 GACCACAGTGTC
EagI 1092 CGGCCG C^GGCCG
1892 CGGCCG
EclXI 1092 CGGCCG C^GGCCG
1892 CGGCCG
Eco52I 1092 CGGCCG C^GGCCG
1892 CGGCCG
EcoNI 384 CCTTCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3288 CCTGGGGGAGG
MfeI 1265 CAATTG C^AATTG
1508 CAATTG
MflI 1340 GGATCC [GA]^GATC[CT]
2616 AGATCT
MunI 1265 CAATTG C^AATTG
1508 CAATTG
PflMI 339 CCAGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1836 CCAAAGCATGG
SacI 2168 GAGCTC GAGCT^C
2653 GAGCTC
SexAI 760 ACCAGGT A^CC[AT]GGT
3239 ACCAGGT
SspI 700 AATATT AAT^ATT
3914 AATATT
Van91I 339 CCAGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1836 CCAAAGCATGG
XhoII 1340 GGATCC [GA]^GATC[CT]
2616 AGATCT
XmnI 306 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
379 GAAGACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbeI BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstXI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SphI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XmaCI XmaI