##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AgeI        
AscI        AseI        AsnI        Asp718I     BamHI       
BciVI       BclI        BglII       BsaWI       BseAI       
BsiWI       BspDI       BspEI       BspHI       BssSI       
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
DraI        DraIII      EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HincII      HindII      HindIII     
HpaI        KpnI        KspI        MfeI        MroI        
MunI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AccI          331  GTCTAC               GT^[AC][GT]AC
AflII        1942  CTTAAG               C^TTAAG
Alw44I       1804  GTGCAC               G^TGCAC
AlwNI        4220  CAGTCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         3365  CCTCAGG              CC^T[ACGT]AGG
ApaLI        1804  GTGCAC               G^TGCAC
Asp700I      2565  GAACGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII        4090  CCTAGG               C^CTAGG
BbvCI        3733  CCTCAGC              CCTCAGC(-5/-2)
BfrI         1942  CTTAAG               C^TTAAG
BlnI         4090  CCTAGG               C^CTAGG
BsaBI         411  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI         4141  GCATTC               GAATGC(1/-1)
BsrDI        2700  CATTGC               GCAATG(2/0)
BstAPI       3899  GCAAATGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       3365  CCTCAGG              CC^T[ACGT]AGG
DrdI         1717  GACGTGCGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       3365  CCTCAGG              CC^T[ACGT]AGG
MamI          411  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI         2090  CATATG               CA^TATG
PstI         2509  CTGCAG               CTGCA^G
PvuII        1090  CAGCTG               CAG^CTG
SacI         3372  GAGCTC               GAGCT^C
ScaI         2324  AGTACT               AGT^ACT
StuI         3993  AGGCCT               AGG^CCT
TfiI         3770  GAATC                G^A[AT]TC
XmnI         2565  GAACGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcsI         3134  AAATTT               [GA]^AATT[CT]
             4271  AAATTC              
AhdI          274  GACGACCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3864  GACCGAGGGTC         
ApoI         3134  AAATTT               [GA]^AATT[CT]
             4271  AAATTC              
AspEI         274  GACGACCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3864  GACCGAGGGTC         
AspI          471  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2434  GACTCTGTC           
BalI          323  TGGCCA               TGG^CCA
             1625  TGGCCA              
BbrPI        1341  CACGTG               CAC^GTG
             1690  CACGTG              
BcgI         1277  GCATCTTCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3845  GCAGGACCCTCG        
Bsp1407I      860  TGTACA               T^GTACA
             2807  TGTACA              
BspLU11I      335  ACATGT               A^CATGT
              608  ACATGT              
BsrGI         860  TGTACA               T^GTACA
             2807  TGTACA              
BstXI        2492  CCAGCAAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3101  CCATCCTGTTGG        
EagI         1235  CGGCCG               C^GGCCG
             2725  CGGCCG              
Eam1105I      274  GACGACCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3864  GACCGAGGGTC         
EclXI        1235  CGGCCG               C^GGCCG
             2725  CGGCCG              
Eco52I       1235  CGGCCG               C^GGCCG
             2725  CGGCCG              
MluI         1817  ACGCGT               A^CGCGT
             2528  ACGCGT              
MluNI         323  TGGCCA               TGG^CCA
             1625  TGGCCA              
MscI          323  TGGCCA               TGG^CCA
             1625  TGGCCA              
NcoI           92  CCATGG               C^CATGG
              326  CCATGG              
PflFI         471  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2434  GACTCTGTC           
PmaCI        1341  CACGTG               CAC^GTG
             1690  CACGTG              
PmlI         1341  CACGTG               CAC^GTG
             1690  CACGTG              
PshAI         786  GACCCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2926  GACCCTGGTC          
SfcI           66  CTGTAG               C^T[GA][CT]AG
             2509  CTGCAG              
SspBI         860  TGTACA               T^GTACA
             2807  TGTACA              
Tth111I       471  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2434  GACTCTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  134 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        Aor51HI     ApaI        AspHI       
AvaI        AvaII       AviII       BanI        BanII       
BbeI        BbsI        BbvI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrFI       BsrI        
BssHII      BssKI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco47III    
EcoNI       EcoO109I    EcoO65I     EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
KasI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NdeII       
NgoMIV      NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       RsaI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfoI        SmaI        
SmlI        StyI        TaqI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII       XmaCI       XmaI