##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AocI ApaI AscI Asp718I AspEI
AspI BamHI BbeI BbrPI BcgI
BclI BglII BlpI Bpu1102I BsaBI
BsaI BsiWI BspDI BsrBI BsrFI
BstEII BstPI BstXI Bsu36I CelII
Cfr10I ClaI CpoI DraII DrdI
Eam1105I Eco81I EcoNI EcoO109I EcoO65I
EcoT22I FbaI FseI KasI KpnI
KspI MamI MfeI MluI MspA1I
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SpeI Sse8387I Tth111I XbaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 4176 GTATAC GT^[AC][GT]AC
AccIII 104 TCCGGA T^CCGGA
Alw44I 1654 GTGCAC G^TGCAC
Aor51HI 2916 AGCGCT AGC^GCT
ApaLI 1654 GTGCAC G^TGCAC
AvaI 47 CCCGGG C^[CT]CG[GA]G
AviII 1396 TGCGCA TGC^GCA
AvrII 2449 CCTAGG C^CTAGG
BalI 2287 TGGCCA TGG^CCA
BanI 1948 GGTGCC G^G[CT][GA]CC
BbsI 2632 GAAGAC GAAGAC(2/6)
BbvCI 326 CCTCAGC CCTCAGC(-5/-2)
BglI 2289 GCCACAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2449 CCTAGG C^CTAGG
BpmI 1203 CTGGAG CTGGAG(16/14)
BpuAI 2632 GAAGAC GAAGAC(2/6)
BseAI 104 TCCGGA T^CCGGA
BsiEI 28 CGGCCG CG[GA][CT]^CG
BsmBI 60 CGTCTC CGTCTC(1/5)
BsmI 2396 GCATTC GAATGC(1/-1)
BsoBI 47 CCCGGG C^[CT]CG[GA]G
BspEI 104 TCCGGA T^CCGGA
BspHI 232 TCATGA T^CATGA
BspLU11I 4197 ACATGT A^CATGT
BssHII 15 GCGCGC G^CGCGC
BssSI 1277 CTCGTG CACGAG(-5/-1)
Bst1107I 4176 GTATAC GTA^TAC
BstAPI 3846 GCATTATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 385 TTCGAA TT^CGAA
BstZ17I 4176 GTATAC GTA^TAC
DraIII 1159 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 527 CCATGG C^C[GA][CT]GG
EagI 28 CGGCCG C^GGCCG
EclXI 28 CGGCCG C^GGCCG
Eco47III 2916 AGCGCT AGC^GCT
Eco52I 28 CGGCCG C^GGCCG
EcoRV 1198 GATATC GAT^ATC
FspI 1396 TGCGCA TGC^GCA
MluNI 2287 TGGCCA TGG^CCA
MroI 104 TCCGGA T^CCGGA
MscI 2287 TGGCCA TGG^CCA
NcoI 527 CCATGG C^CATGG
NdeI 4126 CATATG CA^TATG
NspV 385 TTCGAA TT^CGAA
PacI 3682 TTAATTAA TTAAT^TAA
RcaI 232 TCATGA T^CATGA
SfuI 385 TTCGAA TT^CGAA
SmaI 47 CCCGGG CCC^GGG
SnaBI 1660 TACGTA TAC^GTA
SphI 1450 GCATGC GCATG^C
StuI 2446 AGGCCT AGG^CCT
SwaI 4395 ATTTAAAT ATTT^AAAT
XmaCI 47 CCCGGG C^CCGGG
XmaI 47 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflII 346 CTTAAG C^TTAAG
2761 CTTAAG
AlwNI 882 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
3453 CAGTATCTG
BanII 892 GAGCCC G[GA]GC[CT]^C
1679 GGGCTC
BciVI 718 GGATAC GTATCC(6/5)
1150 GGATAC
BcnI 47 CCCGG CC^[GC]GG
48 CCGGG
BfrI 346 CTTAAG C^TTAAG
2761 CTTAAG
BsaWI 104 TCCGGA [AT]^CCGG[AT]
1788 TCCGGT
BseRI 324 CTCCTC GAGGAG(10/8)
1516 CTCCTC
BsrDI 1581 CATTGC GCAATG(2/0)
2510 GCAATG
EcoRI 391 GAATTC G^AATTC
2086 GAATTC
FauI 98 CCCGC CCCGC(4/6)
125 CCCGC
HaeII 633 GGCGCT [GA]GCGC^[CT]
2916 AGCGCT
HindIII 1891 AAGCTT A^AGCTT
2225 AAGCTT
HpaI 1805 GTTAAC GTT^AAC
4373 GTTAAC
NciI 47 CCCGG CC^[GC]GG
48 CCGGG
PflMI 527 CCATGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3658 CCATTTATTGG
PstI 1622 CTGCAG CTGCA^G
1648 CTGCAG
Van91I 527 CCATGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3658 CCATTTATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AseI
AsnI Asp700I AspHI AvaII BbvI
BfaI BmyI BsaAI BsaHI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspMI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV NspI PleI PshBI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI