##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccI AclI AflIII AgeI
AocI AscI AseI AsnI Asp718I
AspI AvrII BglII BlnI BsaBI
BsiWI BsmBI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
Eco81I HpaI KpnI MamI MfeI
MluI MunI NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI ScaI SexAI
SfuI SgrAI SnaBI SphI SspBI
SspI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AflII 3365 CTTAAG C^TTAAG
AhdI 62 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2741 GTGCAC G^TGCAC
ApaLI 2741 GTGCAC G^TGCAC
Asp700I 2725 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 62 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 270 TGCGCA TGC^GCA
BamHI 1381 GGATCC G^GATCC
BbeI 206 GGCGCC GGCGC^C
BbrPI 584 CACGTG CAC^GTG
BciVI 1613 GTATCC GTATCC(6/5)
BclI 783 TGATCA T^GATCA
BfrI 3365 CTTAAG C^TTAAG
BsaI 3418 GGTCTC GGTCTC(1/5)
DrdI 323 GACCTCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 430 CGGCCG C^GGCCG
Eam1105I 62 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 430 CGGCCG C^GGCCG
Eco52I 430 CGGCCG C^GGCCG
EcoRI 689 GAATTC G^AATTC
FbaI 783 TGATCA T^GATCA
FseI 439 GGCCGGCC GGCCGG^CC
FspI 270 TGCGCA TGC^GCA
HincII 1805 GTCAAC GT[CT]^[GA]AC
HindII 1805 GTCAAC GT[CT]^[GA]AC
HindIII 2301 AAGCTT A^AGCTT
KasI 206 GGCGCC G^GCGCC
KspI 371 CCGCGG CCGC^GG
NaeI 440 GCCGGC GCC^GGC
NarI 206 GGCGCC GG^CGCC
NcoI 945 CCATGG C^CATGG
NgoMIV 440 GCCGGC G^CCGGC
PmaCI 584 CACGTG CAC^GTG
PmlI 584 CACGTG CAC^GTG
SacII 371 CCGCGG CCGC^GG
SapI 2725 GAAGAGC GCTCTTC(1/4)
SbfI 1978 CCTGCAGG CCTGCA^GG
SfiI 2458 GGCCCTGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 206 GGCGCC GGC^GCC
SmaI 2553 CCCGGG CCC^GGG
SpeI 3667 ACTAGT A^CTAGT
Sse8387I 1978 CCTGCAGG CCTGCA^GG
XmaCI 2553 CCCGGG C^CCGGG
XmaI 2553 CCCGGG C^CCGGG
XmnI 2725 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 22 GACGTC GACGT^C
881 GACGTC
AccIII 1182 TCCGGA T^CCGGA
2997 TCCGGA
BbsI 710 GAAGAC GAAGAC(2/6)
884 GTCTTC
BcgI 1363 GCACAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2225 GCATTCTTGTCG
BpuAI 710 GAAGAC GAAGAC(2/6)
884 GTCTTC
BsaAI 584 CACGTG [CT]AC^GT[GA]
987 TACGTG
BsaWI 1182 TCCGGA [AT]^CCGG[AT]
2997 TCCGGA
BseAI 1182 TCCGGA T^CCGGA
2997 TCCGGA
BspEI 1182 TCCGGA T^CCGGA
2997 TCCGGA
BsrBI 1162 CCGCTC CCGCTC(-3/-3)
1463 GAGCGG
BsrDI 3167 GCAATG GCAATG(2/0)
3754 GCAATG
BstEII 1505 GGTGACC G^GT[ACGT]ACC
2310 GGTCACC
BstPI 1505 GGTGACC G^GT[ACGT]ACC
2310 GGTCACC
DraI 2852 TTTAAA TTT^AAA
3819 TTTAAA
DraIII 2313 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3034 CACCCTGTG
DsaI 371 CCGCGG C^C[GA][CT]GG
945 CCATGG
EcoO65I 1505 GGTGACC G^GT[ACGT]ACC
2310 GGTCACC
EcoRV 2942 GATATC GAT^ATC
4123 GATATC
EcoT22I 1820 ATGCAT ATGCA^T
2902 ATGCAT
MroI 1182 TCCGGA T^CCGGA
2997 TCCGGA
NsiI 1820 ATGCAT ATGCA^T
2902 ATGCAT
TaqI 333 TCGA T^CGA
2409 TCGA
TthHB8I 333 TCGA T^CGA
2409 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI Aor51HI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI Eco47III
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII