##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccIII AclI AcsI AflII AgeI
Aor51HI ApoI AscI AseI AsnI
AvrII BfrI BlnI BseAI BsiWI
BspDI BspEI BspLU11I BsrDI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI Eco47III EcoO65I EcoRI
EcoRV EcoT22I FseI HindIII HpaI
MfeI MroI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SapI SbfI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 766 GACGTC GACGT^C
AhdI 4253 GACGTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1228 CCTGAGG CC^T[ACGT]AGG
AspEI 4253 GACGTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1891 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1175 TGCGCA TGC^GCA
BbvCI 2913 CCTCAGC CCTCAGC(-5/-2)
BciVI 4147 GTATCC GTATCC(6/5)
BclI 197 TGATCA T^GATCA
BlpI 4531 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4531 GCTGAGC GC^T[ACGT]AGC
BsmI 1234 GAATGC GAATGC(1/-1)
BspHI 2138 TCATGA T^CATGA
Bsu36I 1228 CCTGAGG CC^T[ACGT]AGG
CelII 4531 GCTGAGC GC^T[ACGT]AGC
EagI 3696 CGGCCG C^GGCCG
Eam1105I 4253 GACGTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3696 CGGCCG C^GGCCG
Eco52I 3696 CGGCCG C^GGCCG
Eco81I 1228 CCTGAGG CC^T[ACGT]AGG
FbaI 197 TGATCA T^GATCA
FspI 1175 TGCGCA TGC^GCA
MluI 1574 ACGCGT A^CGCGT
PflFI 1891 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4424 GACGGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2138 TCATGA T^CATGA
SalI 3832 GTCGAC G^TCGAC
ScaI 123 AGTACT AGT^ACT
SexAI 1073 ACCAGGT A^CC[AT]GGT
SphI 459 GCATGC GCATG^C
StuI 2396 AGGCCT AGG^CCT
Tsp509I 4224 AATT ^AATT
Tth111I 1891 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 656 CCAGGGGCGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 1022 GGTACC G^GTACC
1322 GGTACC
AflIII 1574 ACGCGT A^C[GA][CT]GT
3627 ACACGT
Alw44I 628 GTGCAC G^TGCAC
3966 GTGCAC
ApaLI 628 GTGCAC G^TGCAC
3966 GTGCAC
Asp718I 1022 GGTACC G^GTACC
1322 GGTACC
BbrPI 2521 CACGTG CAC^GTG
3628 CACGTG
BbsI 2767 GAAGAC GAAGAC(2/6)
3030 GAAGAC
BfaI 319 CTAG C^TAG
4300 CTAG
BglII 2270 AGATCT A^GATCT
3104 AGATCT
BpuAI 2767 GAAGAC GAAGAC(2/6)
3030 GAAGAC
BsaBI 690 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
726 GATGTTCATC
BsaWI 161 ACCGGA [AT]^CCGG[AT]
2960 TCCGGT
BsmBI 3516 CGTCTC CGTCTC(1/5)
4422 GAGACG
BssSI 1044 CACGAG CACGAG(-5/-1)
2977 CACGAG
DraI 1900 TTTAAA TTT^AAA
4555 TTTAAA
DraIII 1072 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2818 CACGGGGTG
KpnI 1022 GGTACC GGTAC^C
1322 GGTACC
MaeI 319 CTAG C^TAG
4300 CTAG
MamI 690 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
726 GATGTTCATC
NaeI 1246 GCCGGC GCC^GGC
3698 GCCGGC
NgoMIV 1246 GCCGGC G^CCGGC
3698 GCCGGC
PaeR7I 498 CTCGAG C^TCGAG
2203 CTCGAG
PmaCI 2521 CACGTG CAC^GTG
3628 CACGTG
PmlI 2521 CACGTG CAC^GTG
3628 CACGTG
TfiI 255 GAATC G^A[AT]TC
3237 GAATC
XhoI 498 CTCGAG C^TCGAG
2203 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI Asp700I
AspHI AvaI AvaII BalI BamHI
BanI BanII BbeI BbvI BcgI
BcnI BglI BmyI BpmI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrBI BsrFI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI SspBI StyI
TaqI Tru9I TseI Tsp45I TspRI
TthHB8I Van91I XhoII XmaCI XmaI
XmnI