##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AviII AvrII BciVI
BfrI BlnI BsaBI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
Eam1105I EcoNI FseI FspI HgaI
HindIII HpaI KpnI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SpeI Sse8387I SspBI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 1539 GTCTAC GT^[AC][GT]AC
AcyI 28 GGCGCC G[GA]^CG[CT]C
Aor51HI 498 AGCGCT AGC^GCT
ApaI 1719 GGGCCC GGGCC^C
BamHI 1101 GGATCC G^GATCC
BbeI 28 GGCGCC GGCGC^C
BbrPI 3203 CACGTG CAC^GTG
BcgI 3669 GCAGCGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1854 TGATCA T^GATCA
BsaHI 28 GGCGCC G[GA]^CG[CT]C
BsiEI 114 CGGCCG CG[GA][CT]^CG
BsrBI 1171 GAGCGG CCGCTC(-3/-3)
BsrFI 1476 GCCGGC [GA]^CCGG[CT]
BssHII 128 GCGCGC G^CGCGC
BstEII 553 GGTGACC G^GT[ACGT]ACC
BstPI 553 GGTGACC G^GT[ACGT]ACC
Cfr10I 1476 GCCGGC [GA]^CCGG[CT]
DraI 2334 TTTAAA TTT^AAA
EagI 114 CGGCCG C^GGCCG
EclXI 114 CGGCCG C^GGCCG
Eco47III 498 AGCGCT AGC^GCT
Eco52I 114 CGGCCG C^GGCCG
EcoO65I 553 GGTGACC G^GT[ACGT]ACC
EcoRI 427 GAATTC G^AATTC
EcoRV 3391 GATATC GAT^ATC
EcoT22I 1938 ATGCAT ATGCA^T
FbaI 1854 TGATCA T^GATCA
Hin1I 28 GGCGCC G[GA]^CG[CT]C
KasI 28 GGCGCC G^GCGCC
KspI 1144 CCGCGG CCGC^GG
NaeI 1476 GCCGGC GCC^GGC
NarI 28 GGCGCC GG^CGCC
NcoI 3889 CCATGG C^CATGG
NgoMIV 1476 GCCGGC G^CCGGC
NsiI 1938 ATGCAT ATGCA^T
PmaCI 3203 CACGTG CAC^GTG
PmlI 3203 CACGTG CAC^GTG
PshAI 1571 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1144 CCGCGG CCGC^GG
SfoI 28 GGCGCC GGC^GCC
SmaI 1722 CCCGGG CCC^GGG
SnaBI 3443 TACGTA TAC^GTA
SspI 4067 AATATT AAT^ATT
XmaCI 1722 CCCGGG C^CCGGG
XmaI 1722 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 427 GAATTC [GA]^AATT[CT]
2338 AAATTC
Alw44I 62 GTGCAC G^TGCAC
3826 GTGCAC
ApaLI 62 GTGCAC G^TGCAC
3826 GTGCAC
ApoI 427 GAATTC [GA]^AATT[CT]
2338 AAATTC
Asp700I 1354 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2747 GAAACCATTC
AspI 657 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1533 GACTCTGTC
BbsI 1568 GAAGAC GAAGAC(2/6)
3085 GTCTTC
BglII 2409 AGATCT A^GATCT
3271 AGATCT
BlpI 2272 GCTGAGC GC^T[ACGT]AGC
2834 GCTGAGC
Bpu1102I 2272 GCTGAGC GC^T[ACGT]AGC
2834 GCTGAGC
BpuAI 1568 GAAGAC GAAGAC(2/6)
3085 GTCTTC
CelII 2272 GCTGAGC GC^T[ACGT]AGC
2834 GCTGAGC
DrdI 1151 GACCTCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2809 GACTGCCATGTC
HincII 1457 GTCAAC GT[CT]^[GA]AC
1946 GTTGAC
HindII 1457 GTCAAC GT[CT]^[GA]AC
1946 GTTGAC
PaeR7I 1600 CTCGAG C^TCGAG
2032 CTCGAG
PflFI 657 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1533 GACTCTGTC
SacI 2837 GAGCTC GAGCT^C
3884 GAGCTC
SexAI 1696 ACCTGGT A^CC[AT]GGT
2653 ACCAGGT
Tth111I 657 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1533 GACTCTGTC
XhoI 1600 CTCGAG C^TCGAG
2032 CTCGAG
XmnI 1354 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2747 GAAACCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AocI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII