##################################
# NO CUT ENZYMES (   61 enzymes) #
##################################

AccIII      AclI        AcsI        AflII       Aor51HI     
ApoI        AscI        AseI        AsnI        Asp700I     
AvrII       BamHI       BclI        BfrI        BglII       
BlnI        BsaBI       BseAI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BsrGI       
BstBI       ClaI        DraI        DrdI        EagI        
EclXI       Eco47III    Eco52I      EcoRI       EcoRV       
FbaI        HindIII     HpaI        MamI        MfeI        
MluI        MroI        MunI        NdeI        NheI        
NotI        NspV        PacI        PmeI        PshBI       
Psp1406I    RcaI        SalI        SfiI        SfuI        
SpeI        SspBI       SspI        StuI        SwaI        
XmnI        


###################################
# ONE CUT ENZYMES (   20 enzymes) #
###################################

AatII         824  GACGTC               GACGT^C
BbvCI        2998  CCTCAGC              CCTCAGC(-5/-2)
BspLU11I     1948  ACATGT               A^CATGT
Bst1107I     1388  GTATAC               GTA^TAC
BstYI        1915  AGATCC               [GA]^GATC[CT]
BstZ17I      1388  GTATAC               GTA^TAC
EcoT22I       944  ATGCAT               ATGCA^T
FseI         4542  GGCCGGCC             GGCCGG^CC
MflI         1915  AGATCC               [GA]^GATC[CT]
NsiI          944  ATGCAT               ATGCA^T
PaeR7I       2393  CTCGAG               C^TCGAG
PshAI        3502  GACGTGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI         3687  CGATCG               CGAT^CG
SbfI          547  CCTGCAGG             CCTGCA^GG
ScaI         2409  AGTACT               AGT^ACT
SnaBI        4696  TACGTA               TAC^GTA
Sse8387I      547  CCTGCAGG             CCTGCA^GG
Tsp509I      2422  AATT                 ^AATT
XhoI         2393  CTCGAG               C^TCGAG
XhoII        1915  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AccI          125  GTCTAC               GT^[AC][GT]AC
             1388  GTATAC              
AgeI         3853  ACCGGT               A^CCGGT
             3994  ACCGGT              
AhdI         1673  GACAAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2426  GACGATGAGTC         
AocI         1835  CCTGAGG              CC^T[ACGT]AGG
             4513  CCTGAGG             
AspEI        1673  GACAAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2426  GACGATGAGTC         
AspI         2357  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4389  GACTGTGTC           
BalI          771  TGGCCA               TGG^CCA
             3698  TGGCCA              
BcgI         2915  CGAGTCCTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
               30  GCAGCCACTTCG        
BciVI        4693  GGATAC               GTATCC(6/5)
             2209  GTATCC              
BglI         4538  GCCAGGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             4592  GCCAACTGGGC         
BsmI          841  GAATGC               GAATGC(1/-1)
             5063  GCATTC              
BsrDI        4313  CATTGC               GCAATG(2/0)
             3414  GCAATG              
BssSI        3347  CACGAG               CACGAG(-5/-1)
             2197  CTCGTG              
BstXI        1849  CCAGGACAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4645  CCACACCTATGG        
Bsu36I       1835  CCTGAGG              CC^T[ACGT]AGG
             4513  CCTGAGG             
Eam1105I     1673  GACAAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2426  GACGATGAGTC         
Eco81I       1835  CCTGAGG              CC^T[ACGT]AGG
             4513  CCTGAGG             
HincII       1114  GTTGAC               GT[CT]^[GA]AC
             1684  GTTGAC              
HindII       1114  GTTGAC               GT[CT]^[GA]AC
             1684  GTTGAC              
KspI          210  CCGCGG               CCGC^GG
             3743  CCGCGG              
MluNI         771  TGGCCA               TGG^CCA
             3698  TGGCCA              
MscI          771  TGGCCA               TGG^CCA
             3698  TGGCCA              
MseI         1455  TTAA                 T^TAA
             4640  TTAA                
NaeI         2277  GCCGGC               GCC^GGC
             4543  GCCGGC              
NcoI         3599  CCATGG               C^CATGG
             3636  CCATGG              
NgoMIV       2277  GCCGGC               G^CCGGC
             4543  GCCGGC              
NruI         2488  TCGCGA               TCG^CGA
             4344  TCGCGA              
PflFI        2357  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4389  GACTGTGTC           
PinAI        3853  ACCGGT               A^CCGGT
             3994  ACCGGT              
SacII         210  CCGCGG               CCGC^GG
             3743  CCGCGG              
SapI         1463  GAAGAGC              GCTCTTC(1/4)
             3296  GAAGAGC             
SexAI        1445  ACCTGGT              A^CC[AT]GGT
             1751  ACCTGGT             
SgrAI         234  CGCCGGTG             C[GA]^CCGG[CT]G
             4251  CGCCGGTG            
TfiI          116  GATTC                G^A[AT]TC
             1198  GATTC               
Tru9I        1455  TTAA                 T^TAA
             4640  TTAA                
Tth111I      2357  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4389  GACTGTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  141 enzymes) #
#########################################

Acc65I      AccII       AciI        AcyI        AfaI        
AflIII      AluI        Alw44I      AlwI        AlwNI       
ApaI        ApaLI       Asp718I     AspHI       AvaI        
AvaII       AviII       BanI        BanII       BbeI        
BbrPI       BbsI        BbvI        BcnI        BfaI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssHII      
BssKI       BstAPI      BstEII      BstF5I      BstNI       
BstPI       BstUI       Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      CpoI        DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoO65I     EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
KasI        KpnI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NarI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PmaCI       PmlI        
PpuMI       PstI        PvuII       RsaI        RsrII       
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmaI        SmlI        SphI        
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     Van91I      XbaI        XcmI        XmaCI       
XmaI