##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AccIII AclI AflIII AhdI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BalI BbrPI BcgI
BciVI BglI BlnI BlpI Bpu1102I
BsaAI BseAI BsiWI BspDI BspEI
BspLU11I Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
FseI FspI HindIII MfeI MluI
MluNI MroI MscI MunI NaeI
NdeI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SfiI
SfuI SnaBI Sse8387I StuI SwaI
Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 3306 GACGTC GACGT^C
AflII 4078 CTTAAG C^TTAAG
AgeI 1221 ACCGGT A^CCGGT
Alw44I 2675 GTGCAC G^TGCAC
AocI 3260 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2188 AGCGCT AGC^GCT
ApaLI 2675 GTGCAC G^TGCAC
BamHI 583 GGATCC G^GATCC
BbsI 1155 GTCTTC GAAGAC(2/6)
BclI 4111 TGATCA T^GATCA
BfrI 4078 CTTAAG C^TTAAG
BpuAI 1155 GTCTTC GAAGAC(2/6)
BsaBI 787 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 129 CGGTCG CG[GA][CT]^CG
BssHII 124 GCGCGC G^CGCGC
BssSI 977 CACGAG CACGAG(-5/-1)
BstXI 2644 CCAGCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3260 CCTGAGG CC^T[ACGT]AGG
DraIII 2669 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 975 GACACGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2188 AGCGCT AGC^GCT
Eco81I 3260 CCTGAGG CC^T[ACGT]AGG
EcoRV 1927 GATATC GAT^ATC
FbaI 4111 TGATCA T^GATCA
HpaI 3569 GTTAAC GTT^AAC
KspI 60 CCGCGG CCGC^GG
MamI 787 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 1777 GCTAGC G^CTAGC
PinAI 1221 ACCGGT A^CCGGT
PpuMI 2927 AGGACCC [GA]G^G[AT]CC[CT]
SacI 3946 GAGCTC GAGCT^C
SacII 60 CCGCGG CCGC^GG
SapI 4137 GCTCTTC GCTCTTC(1/4)
SexAI 2650 ACCTGGT A^CC[AT]GGT
SgrAI 143 CACCGGCG C[GA]^CCGG[CT]G
SpeI 3921 ACTAGT A^CTAGT
SphI 2786 GCATGC GCATG^C
TaqI 899 TCGA T^CGA
TthHB8I 899 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AvaI 106 CCCGGG C^[CT]CG[GA]G
167 CCCGGG
BglII 849 AGATCT A^GATCT
2577 AGATCT
BsaWI 1221 ACCGGT [AT]^CCGG[AT]
1791 TCCGGT
BsmBI 1178 CGTCTC CGTCTC(1/5)
1069 GAGACG
BsmI 2589 GAATGC GAATGC(1/-1)
2765 GAATGC
BsoBI 106 CCCGGG C^[CT]CG[GA]G
167 CCCGGG
Bsp1407I 3332 TGTACA T^GTACA
4017 TGTACA
BspHI 336 TCATGA T^CATGA
3770 TCATGA
BsrDI 1732 GCAATG GCAATG(2/0)
2479 GCAATG
BsrGI 3332 TGTACA T^GTACA
4017 TGTACA
EaeI 83 CGGCCA [CT]^GGCC[GA]
419 TGGCCG
HgaI 228 GACGC GACGC(5/10)
1071 GACGC
HincII 2607 GTTGAC GT[CT]^[GA]AC
3569 GTTAAC
HindII 2607 GTTGAC GT[CT]^[GA]AC
3569 GTTAAC
NcoI 588 CCATGG C^CATGG
2426 CCATGG
PstI 1598 CTGCAG CTGCA^G
1967 CTGCAG
RcaI 336 TCATGA T^CATGA
3770 TCATGA
SmaI 106 CCCGGG CCC^GGG
167 CCCGGG
SmlI 994 CTCAAG C^T[CT][GA]AG
4078 CTTAAG
SspBI 3332 TGTACA T^GTACA
4017 TGTACA
XmaCI 106 CCCGGG C^CCGGG
167 CCCGGG
XmaI 106 CCCGGG C^CCGGG
167 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AcyI AfaI AluI AlwI AlwNI
ApaI ApoI Asp718I AspHI AvaII
BanI BanII BbeI BbvCI BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoNI EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XbaI XcmI XhoII