##################################
# NO CUT ENZYMES (   64 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AgeI        
Aor51HI     ApoI        AscI        AseI        AsnI        
Asp718I     AvrII       BciVI       BclI        BglII       
BlnI        BsiWI       BsmI        BspDI       BspHI       
BspLU11I    BssHII      BssSI       Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        Eco47III    EcoRI       
EcoRV       EcoT22I     FbaI        HincII      HindII      
HpaI        KpnI        MfeI        MluI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SalI        SbfI        SfuI        SpeI        
Sse8387I    SspI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   24 enzymes) #
###################################

AflII        4334  CTTAAG               C^TTAAG
Asp700I      1836  GAAGGGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI         3961  CGACACTAATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         1291  CTAG                 C^TAG
BfrI         4334  CTTAAG               C^TTAAG
BlpI          230  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      230  GCTCAGC              GC^T[ACGT]AGC
BsaBI        4347  GATGCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I      600  TGTACA               T^GTACA
BsrDI        3088  GCAATG               GCAATG(2/0)
BsrGI         600  TGTACA               T^GTACA
BstAPI       1187  GCATTTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII         230  GCTCAGC              GC^T[ACGT]AGC
FseI         1996  GGCCGGCC             GGCCGG^CC
MaeI         1291  CTAG                 C^TAG
MamI         4347  GATGCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
ScaI         3606  AGTACT               AGT^ACT
SexAI        3056  ACCTGGT              A^CC[AT]GGT
SfiI         1945  GGCCAGGTCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI         279  TACGTA               TAC^GTA
SphI          397  GCATGC               GCATG^C
SspBI         600  TGTACA               T^GTACA
SwaI          265  ATTTAAAT             ATTT^AAAT
XmnI         1836  GAAGGGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccI         3384  GTAGAC               GT^[AC][GT]AC
             4285  GTAGAC              
AflIII        597  ACGTGT               A^C[GA][CT]GT
              631  ACGTGT              
Alw44I       1230  GTGCAC               G^TGCAC
             3360  GTGCAC              
AocI         3158  CCTGAGG              CC^T[ACGT]AGG
             4078  CCTGAGG             
ApaLI        1230  GTGCAC               G^TGCAC
             3360  GTGCAC              
AviII        1179  TGCGCA               TGC^GCA
             1870  TGCGCA              
BalI          940  TGGCCA               TGG^CCA
             3062  TGGCCA              
BbrPI         596  CACGTG               CAC^GTG
              893  CACGTG              
BglI         1776  GCCAGCCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1946  GCCAGGTCGGC         
BsgI         3539  CTGCAC               GTGCAG(16/14)
              956  GTGCAG              
BsrBI        2586  CCGCTC               CCGCTC(-3/-3)
             1595  GAGCGG              
Bsu36I       3158  CCTGAGG              CC^T[ACGT]AGG
             4078  CCTGAGG             
DraIII       1881  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3055  CACCTGGTG           
DrdI         1323  GACCAGGGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2974  GACGCCCATGTC        
EagI          391  CGGCCG               C^GGCCG
             1995  CGGCCG              
EclXI         391  CGGCCG               C^GGCCG
             1995  CGGCCG              
Eco52I        391  CGGCCG               C^GGCCG
             1995  CGGCCG              
Eco81I       3158  CCTGAGG              CC^T[ACGT]AGG
             4078  CCTGAGG             
FspI         1179  TGCGCA               TGC^GCA
             1870  TGCGCA              
HindIII      2053  AAGCTT               A^AGCTT
             4060  AAGCTT              
KspI           54  CCGCGG               CCGC^GG
              102  CCGCGG              
MluNI         940  TGGCCA               TGG^CCA
             3062  TGGCCA              
MscI          940  TGGCCA               TGG^CCA
             3062  TGGCCA              
NaeI          393  GCCGGC               GCC^GGC
             1997  GCCGGC              
NgoMIV        393  GCCGGC               G^CCGGC
             1997  GCCGGC              
PmaCI         596  CACGTG               CAC^GTG
              893  CACGTG              
PmlI          596  CACGTG               CAC^GTG
              893  CACGTG              
SacII          54  CCGCGG               CCGC^GG
              102  CCGCGG              
StuI         2996  AGGCCT               AGG^CCT
             3535  AGGCCT              
Tsp509I      1696  AATT                 ^AATT
             3622  AATT                
XcmI         1064  CCAGGGGAGGCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2506  CCAGACCCACTGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  139 enzymes) #
#########################################

AccII       AccIII      AciI        AcyI        AfaI        
AhdI        AluI        AlwI        AlwNI       ApaI        
AspEI       AspHI       AspI        AvaI        AvaII       
BamHI       BanI        BanII       BbeI        BbsI        
BbvCI       BbvI        BcnI        BmyI        BpmI        
BpuAI       BsaAI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    BspEI       BspMI       BsrFI       
BsrI        BssKI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EaeI        Eam1105I    
EarI        EcoNI       EcoO109I    EcoO65I     EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
KasI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MroI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PflFI       PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SfoI        SgrAI       SmaI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      TspRI       Tth111I     TthHB8I     
Van91I      XhoII       XmaCI       XmaI