##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
Asp718I AspEI AviII AvrII BalI
BanI BbeI BbrPI BbvCI BcgI
BglI BlnI BpmI BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BsrBI BsrFI BsrGI BssHII
BssSI BstEII BstPI BstXI Bsu36I
Cfr10I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT14I FauI
FseI FspI HgaI HpaI KasI
KpnI MamI MluI MluNI MroI
MscI NaeI NarI NcoI NgoMIV
NheI NotI NruI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SapI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 45 GACGTC GACGT^C
AccII 4 CGCG CG^CG
AcyI 45 GACGTC G[GA]^CG[CT]C
AflII 1730 CTTAAG C^TTAAG
AlwNI 3057 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 30 GGGCCC GGGCC^C
AseI 2659 ATTAAT AT^TAAT
AsnI 2659 ATTAAT AT^TAAT
Asp700I 1930 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 76 GAGCAC G[AT]GC[AT]^C
AvaI 112 CCCGAG C^[CT]CG[GA]G
AvaII 2253 GGACC G^G[AT]CC
BanII 30 GGGCCC G[GA]GC[CT]^C
BciVI 2824 GGATAC GTATCC(6/5)
BfrI 1730 CTTAAG C^TTAAG
BglII 1736 AGATCT A^GATCT
BsaHI 45 GACGTC G[GA]^CG[CT]C
BsgI 241 GTGCAG GTGCAG(16/14)
BsiHKAI 76 GAGCAC G[AT]GC[AT]^C
BsoBI 112 CCCGAG C^[CT]CG[GA]G
BspHI 4239 TCATGA T^CATGA
BstAPI 234 GCAGATTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 980 TTCGAA TT^CGAA
BstUI 4 CGCG CG^CG
CfoI 153 GCGC GCG^C
EaeI 62 TGGCCG [CT]^GGCC[GA]
EcoRI 464 GAATTC G^AATTC
HaeII 152 GGCGCT [GA]GCGC^[CT]
HhaI 153 GCGC GCG^C
Hin1I 45 GACGTC G[GA]^CG[CT]C
HinP1I 153 GCGC G^CGC
HincII 51 GTCGAC GT[CT]^[GA]AC
HindII 51 GTCGAC GT[CT]^[GA]AC
KspI 3 CCGCGG CCGC^GG
MvnI 4 CGCG CG^CG
NspV 980 TTCGAA TT^CGAA
PacI 2601 TTAATTAA TTAAT^TAA
PshBI 2659 ATTAAT AT^TAAT
RcaI 4239 TCATGA T^CATGA
SacII 3 CCGCGG CCGC^GG
SalI 51 GTCGAC G^TCGAC
SexAI 158 ACCAGGT A^CC[AT]GGT
SfuI 980 TTCGAA TT^CGAA
SmlI 1730 CTTAAG C^T[CT][GA]AG
XcmI 138 CCAGCCGGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1930 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 800 ACATGT A^C[GA][CT]GT
2828 ACATGT
AspI 42 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
45 GACGTCGTC
BamHI 108 GGATCC G^GATCC
668 GGATCC
BcnI 118 CCGGG CC^[GC]GG
142 CCGGG
BspLU11I 800 ACATGT A^CATGT
2828 ACATGT
BspMI 1347 ACCTGC ACCTGC(4/8)
3469 ACCTGC
BsrDI 430 GCAATG GCAATG(2/0)
1456 GCAATG
Bst1107I 1534 GTATAC GTA^TAC
2902 GTATAC
BstNI 159 CCAGG CC^[AT]GG
3683 CCTGG
BstZ17I 1534 GTATAC GTA^TAC
2902 GTATAC
DraII 29 GGGGCCC [GA]G^G[ACGT]CC[CT]
30 GGGCCCT
DrdI 42 GACGACGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2041 GACTGTGGGGTC
DsaI 3 CCGCGG C^C[GA][CT]GG
65 CCGTGG
EcoO109I 29 GGGGCCC [GA]G^G[ACGT]CC[CT]
30 GGGCCCT
EcoRII 159 CCAGG ^CC[AT]GG
3683 CCTGG
MaeII 46 ACGT A^CGT
1576 ACGT
MfeI 3535 CAATTG C^AATTG
4078 CAATTG
MunI 3535 CAATTG C^AATTG
4078 CAATTG
MvaI 159 CCAGG CC^[AT]GG
3683 CCTGG
NciI 118 CCGGG CC^[GC]GG
142 CCGGG
PflFI 42 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
45 GACGTCGTC
SspI 2571 AATATT AAT^ATT
3481 AATATT
Tth111I 42 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
45 GACGTCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI BbsI BbvI BclI
BfaI BlpI BmyI Bpu1102I BpuAI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraI EarI EcoT22I FbaI Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MwoI
NdeI NdeII NlaIII NlaIV NsiI
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII