##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AccI AclI AflII AhdI AscI
AspEI AspI AvrII BbrPI BbvCI
BfrI BglII BlnI BsaBI BsiWI
BspDI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I ClaI DrdI Eam1105I
EcoO65I EcoRI EcoT22I FseI HincII
HindII HpaI MamI MfeI MluI
MunI NaeI NdeI NgoMIV NotI
NruI NsiI PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI SalI SbfI ScaI SexAI
SfiI SgrAI SnaBI SpeI Sse8387I
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1692 GACGTC GACGT^C
AccIII 383 TCCGGA T^CCGGA
AgeI 3434 ACCGGT A^CCGGT
Alw44I 3498 GTGCAC G^TGCAC
AocI 2000 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1621 AGCGCT AGC^GCT
ApaLI 3498 GTGCAC G^TGCAC
AseI 3766 ATTAAT AT^TAAT
AsnI 3766 ATTAAT AT^TAAT
BbsI 481 GTCTTC GAAGAC(2/6)
BcgI 203 CGAACTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3336 TGATCA T^GATCA
BpuAI 481 GTCTTC GAAGAC(2/6)
BsaAI 3574 CACGTA [CT]AC^GT[GA]
BseAI 383 TCCGGA T^CCGGA
Bsp1407I 1876 TGTACA T^GTACA
BspEI 383 TCCGGA T^CCGGA
BspHI 3549 TCATGA T^CATGA
BspMI 1906 ACCTGC ACCTGC(4/8)
BsrGI 1876 TGTACA T^GTACA
BstAPI 238 GCAGCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2000 CCTCAGG CC^T[ACGT]AGG
CpoI 2202 CGGACCG CG^G[AT]CCG
DraI 3309 TTTAAA TTT^AAA
EagI 3300 CGGCCG C^GGCCG
EclXI 3300 CGGCCG C^GGCCG
Eco47III 1621 AGCGCT AGC^GCT
Eco52I 3300 CGGCCG C^GGCCG
Eco81I 2000 CCTCAGG CC^T[ACGT]AGG
EcoRV 1161 GATATC GAT^ATC
FbaI 3336 TGATCA T^GATCA
HgaI 1794 GACGC GACGC(5/10)
HindIII 1101 AAGCTT A^AGCTT
KspI 175 CCGCGG CCGC^GG
MroI 383 TCCGGA T^CCGGA
NheI 2542 GCTAGC G^CTAGC
PinAI 3434 ACCGGT A^CCGGT
PshBI 3766 ATTAAT AT^TAAT
RcaI 3549 TCATGA T^CATGA
RsrII 2202 CGGACCG CG^G[AT]CCG
SacII 175 CCGCGG CCGC^GG
SapI 983 GCTCTTC GCTCTTC(1/4)
SmaI 91 CCCGGG CCC^GGG
SspBI 1876 TGTACA T^GTACA
StuI 3524 AGGCCT AGG^CCT
XmaCI 91 CCCGGG C^CCGGG
XmaI 91 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 1258 GGTACC G^GTACC
2152 GGTACC
AcsI 459 AAATTC [GA]^AATT[CT]
2620 AAATTC
ApaI 1764 GGGCCC GGGCC^C
2607 GGGCCC
ApoI 459 AAATTC [GA]^AATT[CT]
2620 AAATTC
Asp718I 1258 GGTACC G^GTACC
2152 GGTACC
AvaI 35 CCCGAG C^[CT]CG[GA]G
91 CCCGGG
AviII 631 TGCGCA TGC^GCA
1912 TGCGCA
BamHI 1060 GGATCC G^GATCC
2710 GGATCC
BbeI 67 GGCGCC GGCGC^C
288 GGCGCC
BciVI 867 GGATAC GTATCC(6/5)
3577 GTATCC
BglI 274 GCCTCGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2494 GCCGCTACGGC
BsaI 2696 GAGACC GGTCTC(1/5)
3588 GAGACC
BsmI 2744 GCATTC GAATGC(1/-1)
3023 GCATTC
BsoBI 35 CCCGAG C^[CT]CG[GA]G
91 CCCGGG
BspLU11I 1948 ACATGT A^CATGT
2485 ACATGT
BsrBI 224 CCGCTC CCGCTC(-3/-3)
1523 CCGCTC
BstBI 531 TTCGAA TT^CGAA
3530 TTCGAA
BstXI 1768 CCATCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3056 CCAACCACTTGG
DraIII 1854 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
1869 CACATCGTG
EcoNI 683 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3681 CCTGTCCAAGG
FspI 631 TGCGCA TGC^GCA
1912 TGCGCA
KasI 67 GGCGCC G^GCGCC
288 GGCGCC
KpnI 1258 GGTACC GGTAC^C
2152 GGTACC
NarI 67 GGCGCC GG^CGCC
288 GGCGCC
NspV 531 TTCGAA TT^CGAA
3530 TTCGAA
PstI 443 CTGCAG CTGCA^G
1675 CTGCAG
SacI 1038 GAGCTC GAGCT^C
1398 GAGCTC
SfoI 67 GGCGCC GGC^GCC
288 GGCGCC
SfuI 531 TTCGAA TT^CGAA
3530 TTCGAA
SmlI 1041 CTCAAG C^T[CT][GA]AG
2463 CTCAAG
SphI 634 GCATGC GCATG^C
1408 GCATGC
SspI 3763 AATATT AAT^ATT
3769 AATATT
XcmI 495 CCAGCCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2593 CCACTCGCTGTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI Asp700I AspHI
AvaII BalI BanI BanII BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaHI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI