##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AgeI AhdI AocI ApaI AscI
AspEI AviII AvrII BamHI BcgI
BglII BlnI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrDI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I Bsu36I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I FseI FspI HgaI
KspI MamI MfeI MluI MroI
MunI MvnI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmeI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI Sse8387I StuI
SwaI Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 1869 GGTACC G^GTACC
AcyI 2381 GGCGCC G[GA]^CG[CT]C
Alw44I 2031 GTGCAC G^TGCAC
Aor51HI 2675 AGCGCT AGC^GCT
ApaLI 2031 GTGCAC G^TGCAC
AseI 4371 ATTAAT AT^TAAT
AsnI 4371 ATTAAT AT^TAAT
Asp718I 1869 GGTACC G^GTACC
AspI 2896 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1015 CTCGGG C^[CT]CG[GA]G
BbeI 2381 GGCGCC GGCGC^C
BbrPI 2581 CACGTG CAC^GTG
BbvCI 1864 GCTGAGG CCTCAGC(-5/-2)
BclI 2358 TGATCA T^GATCA
BcnI 2256 CCGGG CC^[GC]GG
BsaAI 2581 CACGTG [CT]AC^GT[GA]
BsaHI 2381 GGCGCC G[GA]^CG[CT]C
BsaI 4172 GGTCTC GGTCTC(1/5)
BsoBI 1015 CTCGGG C^[CT]CG[GA]G
BspMI 2934 ACCTGC ACCTGC(4/8)
BsrBI 3445 GAGCGG CCGCTC(-3/-3)
BsrFI 3149 GCCGGC [GA]^CCGG[CT]
BstEII 3027 GGTTACC G^GT[ACGT]ACC
BstPI 3027 GGTTACC G^GT[ACGT]ACC
Cfr10I 3149 GCCGGC [GA]^CCGG[CT]
Eco47III 2675 AGCGCT AGC^GCT
EcoO65I 3027 GGTTACC G^GT[ACGT]ACC
EcoRV 4687 GATATC GAT^ATC
FbaI 2358 TGATCA T^GATCA
Hin1I 2381 GGCGCC G[GA]^CG[CT]C
HpaI 3889 GTTAAC GTT^AAC
KasI 2381 GGCGCC G^GCGCC
KpnI 1869 GGTACC GGTAC^C
MaeII 2582 ACGT A^CGT
NaeI 3149 GCCGGC GCC^GGC
NarI 2381 GGCGCC GG^CGCC
NciI 2256 CCGGG CC^[GC]GG
NdeI 474 CATATG CA^TATG
NgoMIV 3149 GCCGGC G^CCGGC
NheI 2167 GCTAGC G^CTAGC
PflFI 2896 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2581 CACGTG CAC^GTG
PmlI 2581 CACGTG CAC^GTG
PpuMI 2716 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 4995 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4371 ATTAAT AT^TAAT
SapI 3532 GCTCTTC GCTCTTC(1/4)
SfoI 2381 GGCGCC GGC^GCC
SpeI 4600 ACTAGT A^CTAGT
SphI 92 GCATGC GCATG^C
Tth111I 2896 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2507 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AflII 1653 CTTAAG C^TTAAG
4260 CTTAAG
BalI 1558 TGGCCA TGG^CCA
1672 TGGCCA
BciVI 276 GTATCC GTATCC(6/5)
4928 GTATCC
BfrI 1653 CTTAAG C^TTAAG
4260 CTTAAG
BglI 2109 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2147 GCCATTCGGGC
FauI 1419 GCGGG CCCGC(4/6)
4456 GCGGG
HaeII 2381 GGCGCC [GA]GCGC^[CT]
2675 AGCGCT
MluNI 1558 TGGCCA TGG^CCA
1672 TGGCCA
MscI 1558 TGGCCA TGG^CCA
1672 TGGCCA
SacI 2682 GAGCTC GAGCT^C
2788 GAGCTC
XcmI 23 CCATTCTCAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2110 CCACCAAGGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BanI BanII BbsI BbvI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspHI BspLU11I BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV NsiI
NspI PleI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI