##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AclI AflII AhdI Aor51HI
AscI AseI AsnI Asp700I AspEI
AviII BbvCI BfrI BglII BsaBI
BsiWI Bsp1407I BspLU11I BsrGI Bst1107I
BstBI BstZ17I CpoI DraI DrdI
Eam1105I Eco47III FspI HindIII HpaI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NspV PacI
PaeR7I PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI ScaI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 3209 GGTACC G^GTACC
AccIII 1445 TCCGGA T^CCGGA
AflIII 4851 ACACGT A^C[GA][CT]GT
AgeI 4592 ACCGGT A^CCGGT
Alw44I 3201 GTGCAC G^TGCAC
AocI 220 CCTCAGG CC^T[ACGT]AGG
ApaLI 3201 GTGCAC G^TGCAC
Asp718I 3209 GGTACC G^GTACC
BcgI 2791 GCAAGAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 639 GGATAC GTATCC(6/5)
BclI 4776 TGATCA T^GATCA
BseAI 1445 TCCGGA T^CCGGA
BsmI 4292 GCATTC GAATGC(1/-1)
BspDI 4011 ATCGAT AT^CGAT
BspEI 1445 TCCGGA T^CCGGA
BsrBI 4734 GAGCGG CCGCTC(-3/-3)
BstEII 1499 GGTGACC G^GT[ACGT]ACC
BstPI 1499 GGTGACC G^GT[ACGT]ACC
Bsu36I 220 CCTCAGG CC^T[ACGT]AGG
ClaI 4011 ATCGAT AT^CGAT
Eco81I 220 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1499 GGTGACC G^GT[ACGT]ACC
EcoRI 516 GAATTC G^AATTC
EcoRV 2049 GATATC GAT^ATC
EcoT22I 4872 ATGCAT ATGCA^T
FbaI 4776 TGATCA T^GATCA
FseI 2498 GGCCGGCC GGCCGG^CC
KpnI 3209 GGTACC GGTAC^C
MroI 1445 TCCGGA T^CCGGA
NsiI 4872 ATGCAT ATGCA^T
PinAI 4592 ACCGGT A^CCGGT
SfiI 2468 GGCCGCGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 3505 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 1398 GTCTAC GT^[AC][GT]AC
4947 GTCTAC
AcsI 516 GAATTC [GA]^AATT[CT]
4526 AAATTC
ApoI 516 GAATTC [GA]^AATT[CT]
4526 AAATTC
AspI 1095 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1701 GACTTTGTC
BalI 3776 TGGCCA TGG^CCA
4318 TGGCCA
BbrPI 3766 CACGTG CAC^GTG
4852 CACGTG
BspHI 755 TCATGA T^CATGA
3226 TCATGA
BssSI 2841 CTCGTG CACGAG(-5/-1)
3772 CTCGTG
BstAPI 3104 GCACCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3854 GCAGGCCCTGC
HincII 818 GTCAAC GT[CT]^[GA]AC
3702 GTTGAC
HindII 818 GTCAAC GT[CT]^[GA]AC
3702 GTTGAC
MluNI 3776 TGGCCA TGG^CCA
4318 TGGCCA
MscI 3776 TGGCCA TGG^CCA
4318 TGGCCA
NcoI 212 CCATGG C^CATGG
3119 CCATGG
PflFI 1095 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1701 GACTTTGTC
PmaCI 3766 CACGTG CAC^GTG
4852 CACGTG
PmlI 3766 CACGTG CAC^GTG
4852 CACGTG
RcaI 755 TCATGA T^CATGA
3226 TCATGA
SapI 3086 GAAGAGC GCTCTTC(1/4)
4128 GCTCTTC
SexAI 3205 ACCAGGT A^CC[AT]GGT
3415 ACCTGGT
Tth111I 1095 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1701 GACTTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII AvrII BamHI BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EagI EarI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SacII
Sau3AI Sau96I SbfI ScrFI SfaNI
SfcI SfoI SmaI SmlI Sse8387I
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI