##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AccIII AclI AgeI AhdI Alw44I
ApaLI AscI AseI AsnI AspEI
AviII AvrII BamHI BcgI BciVI
BlnI BsaBI BsaWI BseAI BsiWI
BspDI BspEI BsrDI Bst1107I BstEII
BstPI BstZ17I ClaI CpoI Eam1105I
EcoO65I EcoRV FspI MamI MfeI
MroI MunI NdeI NheI NotI
NruI PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SalI
SgrAI SnaBI SpeI SspI SwaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 3273 GACGTC GACGT^C
Acc65I 1399 GGTACC G^GTACC
AccI 117 GTCTAC GT^[AC][GT]AC
AflII 183 CTTAAG C^TTAAG
Aor51HI 3221 AGCGCT AGC^GCT
Asp700I 4161 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1399 GGTACC G^GTACC
AspI 3273 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2602 CACGTG CAC^GTG
BclI 1046 TGATCA T^GATCA
BfrI 183 CTTAAG C^TTAAG
BglII 1096 AGATCT A^GATCT
BsiEI 2965 CGGCCG CG[GA][CT]^CG
BsmI 3690 GAATGC GAATGC(1/-1)
Bsp1407I 4608 TGTACA T^GTACA
BspLU11I 3296 ACATGT A^CATGT
BsrGI 4608 TGTACA T^GTACA
BssHII 2456 GCGCGC G^CGCGC
BstBI 4248 TTCGAA TT^CGAA
DrdI 1185 GACAGTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2965 CGGCCG C^GGCCG
EclXI 2965 CGGCCG C^GGCCG
Eco47III 3221 AGCGCT AGC^GCT
Eco52I 2965 CGGCCG C^GGCCG
EcoRI 843 GAATTC G^AATTC
EcoT22I 4411 ATGCAT ATGCA^T
FbaI 1046 TGATCA T^GATCA
FseI 2962 GGCCGGCC GGCCGG^CC
KpnI 1399 GGTACC GGTAC^C
MluI 449 ACGCGT A^CGCGT
NcoI 3726 CCATGG C^CATGG
NsiI 4411 ATGCAT ATGCA^T
NspV 4248 TTCGAA TT^CGAA
PflFI 3273 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2602 CACGTG CAC^GTG
PmlI 2602 CACGTG CAC^GTG
ScaI 3408 AGTACT AGT^ACT
SfiI 2648 GGCCTGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 4248 TTCGAA TT^CGAA
SphI 2797 GCATGC GCATG^C
SspBI 4608 TGTACA T^GTACA
Tth111I 3273 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 884 TCTAGA T^CTAGA
XmnI 4161 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
ApaI 1156 GGGCCC GGGCC^C
2529 GGGCCC
BalI 1057 TGGCCA TGG^CCA
2588 TGGCCA
BfaI 885 CTAG C^TAG
4238 CTAG
BsaAI 2602 CACGTG [CT]AC^GT[GA]
4074 TACGTG
BssSI 2121 CACGAG CACGAG(-5/-1)
978 CTCGTG
BstXI 2643 CCACTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2937 CCACGGATGTGG
DraI 3443 TTTAAA TTT^AAA
4263 TTTAAA
HpaI 255 GTTAAC GTT^AAC
3611 GTTAAC
KspI 2177 CCGCGG CCGC^GG
2510 CCGCGG
MaeI 885 CTAG C^TAG
4238 CTAG
MluNI 1057 TGGCCA TGG^CCA
2588 TGGCCA
MscI 1057 TGGCCA TGG^CCA
2588 TGGCCA
PaeR7I 771 CTCGAG C^TCGAG
2484 CTCGAG
SacII 2177 CCGCGG CCGC^GG
2510 CCGCGG
SmaI 2887 CCCGGG CCC^GGG
3070 CCCGGG
XhoI 771 CTCGAG C^TCGAG
2484 CTCGAG
XmaCI 2887 CCCGGG C^CCGGG
3070 CCCGGG
XmaI 2887 CCCGGG C^CCGGG
3070 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI KasI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SacI SapI Sau3AI Sau96I
SbfI ScrFI SexAI SfaNI SfcI
SfoI SmlI Sse8387I StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII