##################################
# NO CUT ENZYMES (   55 enzymes) #
##################################

AccIII      AclI        AgeI        AhdI        Alw44I      
ApaLI       AscI        AseI        AsnI        AspEI       
AviII       AvrII       BamHI       BcgI        BciVI       
BlnI        BsaBI       BsaWI       BseAI       BsiWI       
BspDI       BspEI       BsrDI       Bst1107I    BstEII      
BstPI       BstZ17I     ClaI        CpoI        Eam1105I    
EcoO65I     EcoRV       FspI        MamI        MfeI        
MroI        MunI        NdeI        NheI        NotI        
NruI        PacI        PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SalI        
SgrAI       SnaBI       SpeI        SspI        SwaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII        3273  GACGTC               GACGT^C
Acc65I       1399  GGTACC               G^GTACC
AccI          117  GTCTAC               GT^[AC][GT]AC
AflII         183  CTTAAG               C^TTAAG
Aor51HI      3221  AGCGCT               AGC^GCT
Asp700I      4161  GAAAAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1399  GGTACC               G^GTACC
AspI         3273  GACGTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        2602  CACGTG               CAC^GTG
BclI         1046  TGATCA               T^GATCA
BfrI          183  CTTAAG               C^TTAAG
BglII        1096  AGATCT               A^GATCT
BsiEI        2965  CGGCCG               CG[GA][CT]^CG
BsmI         3690  GAATGC               GAATGC(1/-1)
Bsp1407I     4608  TGTACA               T^GTACA
BspLU11I     3296  ACATGT               A^CATGT
BsrGI        4608  TGTACA               T^GTACA
BssHII       2456  GCGCGC               G^CGCGC
BstBI        4248  TTCGAA               TT^CGAA
DrdI         1185  GACAGTGCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         2965  CGGCCG               C^GGCCG
EclXI        2965  CGGCCG               C^GGCCG
Eco47III     3221  AGCGCT               AGC^GCT
Eco52I       2965  CGGCCG               C^GGCCG
EcoRI         843  GAATTC               G^AATTC
EcoT22I      4411  ATGCAT               ATGCA^T
FbaI         1046  TGATCA               T^GATCA
FseI         2962  GGCCGGCC             GGCCGG^CC
KpnI         1399  GGTACC               GGTAC^C
MluI          449  ACGCGT               A^CGCGT
NcoI         3726  CCATGG               C^CATGG
NsiI         4411  ATGCAT               ATGCA^T
NspV         4248  TTCGAA               TT^CGAA
PflFI        3273  GACGTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        2602  CACGTG               CAC^GTG
PmlI         2602  CACGTG               CAC^GTG
ScaI         3408  AGTACT               AGT^ACT
SfiI         2648  GGCCTGGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI         4248  TTCGAA               TT^CGAA
SphI         2797  GCATGC               GCATG^C
SspBI        4608  TGTACA               T^GTACA
Tth111I      3273  GACGTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          884  TCTAGA               T^CTAGA
XmnI         4161  GAAAAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

ApaI         1156  GGGCCC               GGGCC^C
             2529  GGGCCC              
BalI         1057  TGGCCA               TGG^CCA
             2588  TGGCCA              
BfaI          885  CTAG                 C^TAG
             4238  CTAG                
BsaAI        2602  CACGTG               [CT]AC^GT[GA]
             4074  TACGTG              
BssSI        2121  CACGAG               CACGAG(-5/-1)
              978  CTCGTG              
BstXI        2643  CCACTGGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2937  CCACGGATGTGG        
DraI         3443  TTTAAA               TTT^AAA
             4263  TTTAAA              
HpaI          255  GTTAAC               GTT^AAC
             3611  GTTAAC              
KspI         2177  CCGCGG               CCGC^GG
             2510  CCGCGG              
MaeI          885  CTAG                 C^TAG
             4238  CTAG                
MluNI        1057  TGGCCA               TGG^CCA
             2588  TGGCCA              
MscI         1057  TGGCCA               TGG^CCA
             2588  TGGCCA              
PaeR7I        771  CTCGAG               C^TCGAG
             2484  CTCGAG              
SacII        2177  CCGCGG               CCGC^GG
             2510  CCGCGG              
SmaI         2887  CCCGGG               CCC^GGG
             3070  CCCGGG              
XhoI          771  CTCGAG               C^TCGAG
             2484  CTCGAG              
XmaCI        2887  CCCGGG               C^CCGGG
             3070  CCCGGG              
XmaI         2887  CCCGGG               C^CCGGG
             3070  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  141 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       AocI        
ApoI        AspHI       AvaI        AvaII       BanI        
BanII       BbeI        BbsI        BbvCI       BbvI        
BcnI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspHI       BspMI       BsrBI       BsrFI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstYI       Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DraIII      DsaI        EaeI        EarI        Eco81I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NdeII       
NgoMIV      NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RcaI        
RsaI        SacI        SapI        Sau3AI      Sau96I      
SbfI        ScrFI       SexAI       SfaNI       SfcI        
SfoI        SmlI        Sse8387I    StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII