##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflIII AgeI AhdI Aor51HI ApaI
AscI AspEI AspI AvaI AviII
AvrII BbeI BbrPI BbvCI BcgI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsoBI BspDI BspEI BspLU11I BsrFI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoT22I FseI FspI Hin1I
KasI KspI MamI MluI MroI
MvnI NaeI NarI NcoI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI Psp1406I PstI PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 717 GGTACC G^GTACC
Alw44I 1939 GTGCAC G^TGCAC
ApaLI 1939 GTGCAC G^TGCAC
Asp718I 717 GGTACC G^GTACC
BalI 609 TGGCCA TGG^CCA
BamHI 39 GGATCC G^GATCC
BglI 1044 GCCACTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 4070 CTCCAG CTGGAG(16/14)
BsgI 2168 CTGCAC GTGCAG(16/14)
BsmI 2848 GAATGC GAATGC(1/-1)
Bsp1407I 3302 TGTACA T^GTACA
BsrBI 61 GAGCGG CCGCTC(-3/-3)
BsrGI 3302 TGTACA T^GTACA
BstEII 4594 GGTGACC G^GT[ACGT]ACC
BstPI 4594 GGTGACC G^GT[ACGT]ACC
BstXI 4628 CCATACACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 972 GCGC GCG^C
DraII 2268 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 609 TGGCCA [CT]^GGCC[GA]
EcoNI 5058 CCTCTGATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2268 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 4594 GGTGACC G^GT[ACGT]ACC
EcoRI 5234 GAATTC G^AATTC
EcoRV 1078 GATATC GAT^ATC
EcoT14I 3267 CCAAGG C^C[AT][AT]GG
FauI 3015 GCGGG CCCGC(4/6)
HaeII 971 AGCGCC [GA]GCGC^[CT]
HgaI 4289 GACGC GACGC(5/10)
HhaI 972 GCGC GCG^C
HinP1I 972 GCGC G^CGC
HincII 3508 GTTAAC GT[CT]^[GA]AC
HindII 3508 GTTAAC GT[CT]^[GA]AC
HpaI 3508 GTTAAC GTT^AAC
KpnI 717 GGTACC GGTAC^C
MluNI 609 TGGCCA TGG^CCA
MscI 609 TGGCCA TGG^CCA
NheI 1117 GCTAGC G^CTAGC
PpuMI 2268 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 2571 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SphI 3033 GCATGC GCATG^C
SspBI 3302 TGTACA T^GTACA
StuI 2892 AGGCCT AGG^CCT
StyI 3267 CCAAGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 640 GTAGAC GT^[AC][GT]AC
4687 GTAGAC
AocI 1192 CCTGAGG CC^T[ACGT]AGG
3901 CCTGAGG
Asp700I 95 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1746 GAAGAAATTC
BanI 717 GGTACC G^G[CT][GA]CC
5004 GGCACC
BbsI 4235 GAAGAC GAAGAC(2/6)
3675 GTCTTC
BglII 2493 AGATCT A^GATCT
4839 AGATCT
BpuAI 4235 GAAGAC GAAGAC(2/6)
3675 GTCTTC
BsaI 2329 GAGACC GGTCTC(1/5)
4963 GGTCTC
BsmBI 204 GAGACG CGTCTC(1/5)
3710 GAGACG
BstAPI 2161 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2929 GCAATCTGTGC
Bsu36I 1192 CCTGAGG CC^T[ACGT]AGG
3901 CCTGAGG
DraIII 3306 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
4144 CACCGAGTG
DrdI 2270 GACCCCCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4647 GACTATGGAGTC
Eco81I 1192 CCTGAGG CC^T[ACGT]AGG
3901 CCTGAGG
HindIII 4482 AAGCTT A^AGCTT
4521 AAGCTT
MaeII 2736 ACGT A^CGT
4605 ACGT
MfeI 4196 CAATTG C^AATTG
4916 CAATTG
MunI 4196 CAATTG C^AATTG
4916 CAATTG
SapI 2969 GAAGAGC GCTCTTC(1/4)
4018 GAAGAGC
ScaI 1800 AGTACT AGT^ACT
3346 AGTACT
SfcI 1517 CTACAG C^T[GA][CT]AG
4406 CTACAG
SwaI 942 ATTTAAAT ATTT^AAAT
1562 ATTTAAAT
XmnI 95 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1746 GAAGAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AvaII BanII BbvI BciVI
BclI BcnI BfaI BfrI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRII FbaI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PleI PshBI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SmlI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII