##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflIII      
AgeI        AhdI        Aor51HI     AscI        Asp718I     
AspEI       AviII       AvrII       BalI        BamHI       
BbrPI       BbvCI       BglI        BglII       BlnI        
BsaAI       BsaBI       BsiWI       BsmBI       BsmI        
BspLU11I    Bst1107I    BstBI       BstZ17I     CpoI        
DrdI        Eam1105I    Eco47III    EcoRV       FspI        
HpaI        KpnI        MamI        MluI        MluNI       
MscI        NdeI        NheI        NotI        NruI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       Psp1406I    
PvuI        RsrII       SalI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
Sse8387I    XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AlwNI         320  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI          667  ATTAAT               AT^TAAT
AsnI          667  ATTAAT               AT^TAAT
Asp700I      1441  GAAACCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         4420  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI            2  GGCGCC               GGCGC^C
BcgI         3592  GCAATCCGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        3217  GTATCC               GTATCC(6/5)
BclI         2601  TGATCA               T^GATCA
Bsp1407I     3804  TGTACA               T^GTACA
BspDI         514  ATCGAT               AT^CGAT
BspHI        2637  TCATGA               T^CATGA
BsrBI          71  GAGCGG               CCGCTC(-3/-3)
BsrGI        3804  TGTACA               T^GTACA
BssHII        126  GCGCGC               G^CGCGC
BssSI        2495  CACGAG               CACGAG(-5/-1)
BstAPI       2745  GCATCAAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       3630  GGTTACC              G^GT[ACGT]ACC
BstPI        3630  GGTTACC              G^GT[ACGT]ACC
ClaI          514  ATCGAT               AT^CGAT
DraII          23  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoNI         116  CCTCGGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       23  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I      3630  GGTTACC              G^GT[ACGT]ACC
FbaI         2601  TGATCA               T^GATCA
FseI           56  GGCCGGCC             GGCCGG^CC
HindIII      4794  AAGCTT               A^AGCTT
KasI            2  GGCGCC               G^GCGCC
KspI            7  CCGCGG               CCGC^GG
MaeII         386  ACGT                 A^CGT
MfeI         4133  CAATTG               C^AATTG
MunI         4133  CAATTG               C^AATTG
NarI            2  GGCGCC               GG^CGCC
PflFI        4420  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI         667  ATTAAT               AT^TAAT
RcaI         2637  TCATGA               T^CATGA
SacI         2766  GAGCTC               GAGCT^C
SacII           7  CCGCGG               CCGC^GG
SfoI            2  GGCGCC               GGC^GCC
SmaI           45  CCCGGG               CCC^GGG
SpeI         4195  ACTAGT               A^CTAGT
SphI         2613  GCATGC               GCATG^C
SspBI        3804  TGTACA               T^GTACA
StuI         1162  AGGCCT               AGG^CCT
SwaI         2886  ATTTAAAT             ATTT^AAAT
Tth111I      4420  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         4735  TCTAGA               T^CTAGA
XmaCI          45  CCCGGG               C^CCGGG
XmaI           45  CCCGGG               C^CCGGG
XmnI         1441  GAAACCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

AccIII       3063  TCCGGA               T^CCGGA
             4728  TCCGGA              
AcyI            2  GGCGCC               G[GA]^CG[CT]C
              244  GACGCC              
AfaI         2086  GTAC                 GT^AC
             3805  GTAC                
AocI         1420  CCTGAGG              CC^T[ACGT]AGG
             1531  CCTGAGG             
ApaI           24  GGGCCC               GGGCC^C
             1869  GGGCCC              
BlpI           95  GCTCAGC              GC^T[ACGT]AGC
             1254  GCTCAGC             
Bpu1102I       95  GCTCAGC              GC^T[ACGT]AGC
             1254  GCTCAGC             
BsaHI           2  GGCGCC               G[GA]^CG[CT]C
              244  GACGCC              
BsaWI        3063  TCCGGA               [AT]^CCGG[AT]
             4728  TCCGGA              
BseAI        3063  TCCGGA               T^CCGGA
             4728  TCCGGA              
BseRI        3173  CTCCTC               GAGGAG(10/8)
             1423  GAGGAG              
BsiEI          59  CGGCCG               CG[GA][CT]^CG
              222  CGGCCG              
BspEI        3063  TCCGGA               T^CCGGA
             4728  TCCGGA              
BsrDI        1724  GCAATG               GCAATG(2/0)
             1823  GCAATG              
BsrFI          57  GCCGGC               [GA]^CCGG[CT]
              147  GCCGGC              
BstYI        2334  AGATCC               [GA]^GATC[CT]
             2352  AGATCC              
Bsu36I       1420  CCTGAGG              CC^T[ACGT]AGG
             1531  CCTGAGG             
CelII          95  GCTCAGC              GC^T[ACGT]AGC
             1254  GCTCAGC             
Cfr10I         57  GCCGGC               [GA]^CCGG[CT]
              147  GCCGGC              
EagI           59  CGGCCG               C^GGCCG
              222  CGGCCG              
EclXI          59  CGGCCG               C^GGCCG
              222  CGGCCG              
Eco52I         59  CGGCCG               C^GGCCG
              222  CGGCCG              
Eco81I       1420  CCTGAGG              CC^T[ACGT]AGG
             1531  CCTGAGG             
EcoT22I       713  ATGCAT               ATGCA^T
             2611  ATGCAT              
HaeII           2  GGCGCC               [GA]GCGC^[CT]
              201  AGCGCC              
Hin1I           2  GGCGCC               G[GA]^CG[CT]C
              244  GACGCC              
HincII        868  GTCAAC               GT[CT]^[GA]AC
             1840  GTTGAC              
HindII        868  GTCAAC               GT[CT]^[GA]AC
             1840  GTTGAC              
MflI         2334  AGATCC               [GA]^GATC[CT]
             2352  AGATCC              
MroI         3063  TCCGGA               T^CCGGA
             4728  TCCGGA              
NaeI           57  GCCGGC               GCC^GGC
              147  GCCGGC              
NcoI         1754  CCATGG               C^CATGG
             2783  CCATGG              
NgoMIV         57  GCCGGC               G^CCGGC
              147  GCCGGC              
NsiI          713  ATGCAT               ATGCA^T
             2611  ATGCAT              
RsaI         2086  GTAC                 GT^AC
             3805  GTAC                
SapI         2698  GAAGAGC              GCTCTTC(1/4)
             4723  GCTCTTC             
TaqI          515  TCGA                 T^CGA
             2563  TCGA                
TthHB8I       515  TCGA                 T^CGA
             2563  TCGA                
XhoII        2334  AGATCC               [GA]^GATC[CT]
             2352  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (  101 enzymes) #
#########################################

AccII       AciI        AcsI        AflII       AluI        
Alw44I      AlwI        ApaLI       ApoI        AspHI       
AvaI        AvaII       BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BfrI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraIII      DsaI        EaeI        EarI        EcoRI       
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PstI        
PvuII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Van91I