##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AclI AflIII AgeI AhdI AseI
AsnI AspEI BciVI BclI BglII
BsaBI BsiWI BsmI BspDI BspLU11I
BsrDI ClaI CpoI DraI Eam1105I
FbaI FseI HincII HindII HindIII
HpaI MamI MfeI MluI MunI
NotI NruI PacI PinAI PmeI
PshBI Psp1406I PvuI RsrII SalI
SapI SbfI ScaI SnaBI SpeI
SphI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 4192 GACGTC GACGT^C
Acc65I 4338 GGTACC G^GTACC
AcsI 916 GAATTC [GA]^AATT[CT]
AflII 3160 CTTAAG C^TTAAG
AocI 3139 CCTGAGG CC^T[ACGT]AGG
ApoI 916 GAATTC [GA]^AATT[CT]
AscI 4000 GGCGCGCC GG^CGCGCC
Asp700I 1152 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4338 GGTACC G^GTACC
AspI 735 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2530 GGATCC G^GATCC
BfrI 3160 CTTAAG C^TTAAG
Bst1107I 4789 GTATAC GTA^TAC
BstBI 3199 TTCGAA TT^CGAA
BstZ17I 4789 GTATAC GTA^TAC
Bsu36I 3139 CCTGAGG CC^T[ACGT]AGG
DrdI 3007 GACACGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3139 CCTGAGG CC^T[ACGT]AGG
EcoRI 916 GAATTC G^AATTC
EcoRV 1309 GATATC GAT^ATC
EcoT22I 2654 ATGCAT ATGCA^T
KpnI 4338 GGTACC GGTAC^C
KspI 2525 CCGCGG CCGC^GG
MseI 3161 TTAA T^TAA
NdeI 4794 CATATG CA^TATG
NheI 1485 GCTAGC G^CTAGC
NsiI 2654 ATGCAT ATGCA^T
NspV 3199 TTCGAA TT^CGAA
PflFI 735 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 424 GAGCTC GAGCT^C
SacII 2525 CCGCGG CCGC^GG
SexAI 1205 ACCTGGT A^CC[AT]GGT
SfiI 2501 GGCCTCCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 3199 TTCGAA TT^CGAA
SgrAI 298 CGCCGGTG C[GA]^CCGG[CT]G
SspI 1432 AATATT AAT^ATT
Tru9I 3161 TTAA T^TAA
Tth111I 735 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1152 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 2349 TCCGGA T^CCGGA
3323 TCCGGA
AlwNI 2780 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
4390 CAGCCTCTG
ApaI 2268 GGGCCC GGGCC^C
2546 GGGCCC
AvrII 207 CCTAGG C^CTAGG
3486 CCTAGG
BalI 4247 TGGCCA TGG^CCA
4259 TGGCCA
BbrPI 2089 CACGTG CAC^GTG
3103 CACGTG
BbsI 987 GAAGAC GAAGAC(2/6)
2328 GAAGAC
BcgI 3344 CGAACCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3555 GCACCACATTCG
BlnI 207 CCTAGG C^CTAGG
3486 CCTAGG
BpuAI 987 GAAGAC GAAGAC(2/6)
2328 GAAGAC
BsaAI 2089 CACGTG [CT]AC^GT[GA]
3103 CACGTG
BsaWI 2349 TCCGGA [AT]^CCGG[AT]
3323 TCCGGA
BseAI 2349 TCCGGA T^CCGGA
3323 TCCGGA
Bsp1407I 4777 TGTACA T^GTACA
4798 TGTACA
BspEI 2349 TCCGGA T^CCGGA
3323 TCCGGA
BspHI 1607 TCATGA T^CATGA
2798 TCATGA
BsrGI 4777 TGTACA T^GTACA
4798 TGTACA
BssSI 2209 CACGAG CACGAG(-5/-1)
4291 CACGAG
BstAPI 1346 GCATGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3426 GCACGGGCTGC
BstEII 202 GGTCACC G^GT[ACGT]ACC
2623 GGTGACC
BstPI 202 GGTCACC G^GT[ACGT]ACC
2623 GGTGACC
BstYI 1283 AGATCC [GA]^GATC[CT]
2530 GGATCC
EcoNI 278 CCTCCCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
942 CCTGCTGGAGG
EcoO65I 202 GGTCACC G^GT[ACGT]ACC
2623 GGTGACC
MflI 1283 AGATCC [GA]^GATC[CT]
2530 GGATCC
MluNI 4247 TGGCCA TGG^CCA
4259 TGGCCA
MroI 2349 TCCGGA T^CCGGA
3323 TCCGGA
MscI 4247 TGGCCA TGG^CCA
4259 TGGCCA
NcoI 1559 CCATGG C^CATGG
3933 CCATGG
PaeR7I 3519 CTCGAG C^TCGAG
3898 CTCGAG
PflMI 939 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3708 CCACACGGTGG
PmaCI 2089 CACGTG CAC^GTG
3103 CACGTG
PmlI 2089 CACGTG CAC^GTG
3103 CACGTG
PpuMI 1684 GGGACCC [GA]G^G[AT]CC[CT]
3089 GGGACCT
PshAI 4037 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4611 GACCCCAGTC
RcaI 1607 TCATGA T^CATGA
2798 TCATGA
SspBI 4777 TGTACA T^GTACA
4798 TGTACA
Van91I 939 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3708 CCACACGGTGG
XhoI 3519 CTCGAG C^TCGAG
3898 CTCGAG
XhoII 1283 AGATCC [GA]^GATC[CT]
2530 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI Alw44I AlwI Aor51HI ApaLI
AspHI AvaI AvaII AviII BanI
BanII BbeI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI BstXI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EarI EclXI Eco47III
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XmaCI XmaI