##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
BfrI BsaBI BsiWI Bsp1407I BspHI
BsrGI BssHII Bst1107I BstBI BstZ17I
CpoI DraI Eco47III EcoRI EcoRV
EcoT22I HpaI KspI MamI MfeI
MluI MunI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I RcaI RsrII
SacII ScaI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 406 TCCGGA T^CCGGA
AflIII 1389 ACATGT A^C[GA][CT]GT
AhdI 3072 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3072 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4018 TGCGCA TGC^GCA
BbrPI 963 CACGTG CAC^GTG
BbsI 1551 GTCTTC GAAGAC(2/6)
BcgI 3341 GCAAGGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3778 TGATCA T^GATCA
BpuAI 1551 GTCTTC GAAGAC(2/6)
BseAI 406 TCCGGA T^CCGGA
BsmBI 1968 GAGACG CGTCTC(1/5)
BsmI 4021 GCATTC GAATGC(1/-1)
BspDI 447 ATCGAT AT^CGAT
BspEI 406 TCCGGA T^CCGGA
BspLU11I 1389 ACATGT A^CATGT
BstEII 440 GGTGACC G^GT[ACGT]ACC
BstPI 440 GGTGACC G^GT[ACGT]ACC
ClaI 447 ATCGAT AT^CGAT
DrdI 1599 GACCCCTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1878 CGGCCG C^GGCCG
Eam1105I 3072 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1878 CGGCCG C^GGCCG
Eco52I 1878 CGGCCG C^GGCCG
EcoO65I 440 GGTGACC G^GT[ACGT]ACC
FbaI 3778 TGATCA T^GATCA
FseI 1875 GGCCGGCC GGCCGG^CC
FspI 4018 TGCGCA TGC^GCA
HincII 1614 GTCGAC GT[CT]^[GA]AC
HindII 1614 GTCGAC GT[CT]^[GA]AC
HindIII 999 AAGCTT A^AGCTT
MroI 406 TCCGGA T^CCGGA
NcoI 931 CCATGG C^CATGG
NdeI 2692 CATATG CA^TATG
PaeR7I 3349 CTCGAG C^TCGAG
PmaCI 963 CACGTG CAC^GTG
PmlI 963 CACGTG CAC^GTG
PvuI 2223 CGATCG CGAT^CG
SacI 3440 GAGCTC GAGCT^C
SalI 1614 GTCGAC G^TCGAC
SbfI 4810 CCTGCAGG CCTGCA^GG
SexAI 3075 ACCAGGT A^CC[AT]GGT
SfiI 3345 GGCCCTCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 890 GCATGC GCATG^C
Sse8387I 4810 CCTGCAGG CCTGCA^GG
XhoI 3349 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 2635 GGTACC G^GTACC
2857 GGTACC
AccI 1290 GTCTAC GT^[AC][GT]AC
1614 GTCGAC
AcsI 1086 AAATTC [GA]^AATT[CT]
4993 GAATTT
AocI 3249 CCTCAGG CC^T[ACGT]AGG
4629 CCTTAGG
ApoI 1086 AAATTC [GA]^AATT[CT]
4993 GAATTT
Asp700I 398 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4280 GAAGCTCTTC
Asp718I 2635 GGTACC G^GTACC
2857 GGTACC
AspI 3106 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4145 GACAGAGTC
AvrII 366 CCTAGG C^CTAGG
1922 CCTAGG
BamHI 591 GGATCC G^GATCC
2172 GGATCC
BglII 1039 AGATCT A^GATCT
4381 AGATCT
BlnI 366 CCTAGG C^CTAGG
1922 CCTAGG
BlpI 375 GCTGAGC GC^T[ACGT]AGC
2819 GCTGAGC
Bpu1102I 375 GCTGAGC GC^T[ACGT]AGC
2819 GCTGAGC
BsaAI 693 TACGTG [CT]AC^GT[GA]
963 CACGTG
BsiEI 1878 CGGCCG CG[GA][CT]^CG
2223 CGATCG
BsrBI 657 CCGCTC CCGCTC(-3/-3)
666 GAGCGG
Bsu36I 3249 CCTCAGG CC^T[ACGT]AGG
4629 CCTTAGG
CelII 375 GCTGAGC GC^T[ACGT]AGC
2819 GCTGAGC
Eco81I 3249 CCTCAGG CC^T[ACGT]AGG
4629 CCTTAGG
KpnI 2635 GGTACC GGTAC^C
2857 GGTACC
NaeI 1856 GCCGGC GCC^GGC
1876 GCCGGC
NgoMIV 1856 GCCGGC G^CCGGC
1876 GCCGGC
PflFI 3106 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4145 GACAGAGTC
TfiI 50 GAATC G^A[AT]TC
4931 GATTC
Tsp509I 1087 AATT ^AATT
4994 AATT
Tth111I 3106 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4145 GACAGAGTC
XmnI 398 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4280 GAAGCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbvCI BbvI BciVI BcnI BfaI
BglI BmyI BpmI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI