##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AclI AcyI AgeI Aor51HI
AscI AvaI BbeI BbrPI BcgI
BsaHI BsaI BsiEI BsiWI BsmBI
BsmI BsoBI BsrFI BstAPI BstBI
Cfr10I CpoI DrdI DsaI EagI
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FseI Hin1I KasI KspI
MluI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SfiI SfoI SfuI SgrAI SmaI
SpeI StuI SwaI Van91I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 4303 GGTACC G^GTACC
AccIII 362 TCCGGA T^CCGGA
AhdI 4768 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1119 GTGCAC G^TGCAC
AocI 3275 CCTGAGG CC^T[ACGT]AGG
ApaI 903 GGGCCC GGGCC^C
ApaLI 1119 GTGCAC G^TGCAC
Asp718I 4303 GGTACC G^GTACC
AspEI 4768 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1119 GTGCAC G[AT]GC[AT]^C
AspI 2965 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2217 TGCGCA TGC^GCA
BamHI 395 GGATCC G^GATCC
BciVI 5149 GGATAC GTATCC(6/5)
BclI 3926 TGATCA T^GATCA
BglI 4650 GCCACCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2572 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2572 GCTAAGC GC^T[ACGT]AGC
BsaAI 1845 TACGTA [CT]AC^GT[GA]
BsaWI 362 TCCGGA [AT]^CCGG[AT]
BseAI 362 TCCGGA T^CCGGA
BsgI 935 CTGCAC GTGCAG(16/14)
BsiHKAI 1119 GTGCAC G[AT]GC[AT]^C
BspDI 3453 ATCGAT AT^CGAT
BspEI 362 TCCGGA T^CCGGA
BspLU11I 1540 ACATGT A^CATGT
BspMI 4243 ACCTGC ACCTGC(4/8)
BsrBI 4343 GAGCGG CCGCTC(-3/-3)
BssHII 4093 GCGCGC G^CGCGC
BssSI 3108 CTCGTG CACGAG(-5/-1)
Bst1107I 5071 GTATAC GTA^TAC
BstEII 4782 GGTTACC G^GT[ACGT]ACC
BstPI 4782 GGTTACC G^GT[ACGT]ACC
BstZ17I 5071 GTATAC GTA^TAC
Bsu36I 3275 CCTGAGG CC^T[ACGT]AGG
CelII 2572 GCTAAGC GC^T[ACGT]AGC
ClaI 3453 ATCGAT AT^CGAT
DraIII 4111 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 4768 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3275 CCTGAGG CC^T[ACGT]AGG
EcoNI 2214 CCTTGCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4782 GGTTACC G^GT[ACGT]ACC
FbaI 3926 TGATCA T^GATCA
FspI 2217 TGCGCA TGC^GCA
HaeII 331 AGCGCC [GA]GCGC^[CT]
HgaI 4103 GCGTC GACGC(5/10)
HindIII 3269 AAGCTT A^AGCTT
HpaI 4966 GTTAAC GTT^AAC
KpnI 4303 GGTACC GGTAC^C
MfeI 3636 CAATTG C^AATTG
MroI 362 TCCGGA T^CCGGA
MunI 3636 CAATTG C^AATTG
PflFI 2965 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 2940 GACTC GAGTC(4/5)
SbfI 1933 CCTGCAGG CCTGCA^GG
ScaI 1801 AGTACT AGT^ACT
SnaBI 1845 TACGTA TAC^GTA
SphI 1308 GCATGC GCATG^C
Sse8387I 1933 CCTGCAGG CCTGCA^GG
Tth111I 2965 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4862 TCTAGA T^CTAGA
XcmI 4748 CCAGACATCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 4092 CGCG CG^CG
4094 CGCG
AflII 2834 CTTAAG C^TTAAG
4741 CTTAAG
AflIII 347 ACGTGT A^C[GA][CT]GT
1540 ACATGT
AlwNI 1083 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
2804 CAGCAGCTG
AvrII 3035 CCTAGG C^CTAGG
3585 CCTAGG
BalI 641 TGGCCA TGG^CCA
4648 TGGCCA
BbvCI 676 CCTCAGC CCTCAGC(-5/-2)
1257 GCTGAGG
BcnI 4113 CCCGG CC^[GC]GG
4772 CCCGG
BfrI 2834 CTTAAG C^TTAAG
4741 CTTAAG
BlnI 3035 CCTAGG C^CTAGG
3585 CCTAGG
BsaBI 954 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3921 GATTTTGATC
BseRI 4248 CTCCTC GAGGAG(10/8)
4619 GAGGAG
Bsp1407I 985 TGTACA T^GTACA
4409 TGTACA
BsrGI 985 TGTACA T^GTACA
4409 TGTACA
BstUI 4092 CGCG CG^CG
4094 CGCG
BstXI 69 CCAGTAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4822 CCACTGAGATGG
EaeI 641 TGGCCA [CT]^GGCC[GA]
4648 TGGCCA
HincII 255 GTCAAC GT[CT]^[GA]AC
4966 GTTAAC
HindII 255 GTCAAC GT[CT]^[GA]AC
4966 GTTAAC
MamI 954 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3921 GATTTTGATC
MluNI 641 TGGCCA TGG^CCA
4648 TGGCCA
MscI 641 TGGCCA TGG^CCA
4648 TGGCCA
MvnI 4092 CGCG CG^CG
4094 CGCG
NciI 4113 CCCGG CC^[GC]GG
4772 CCCGG
PpuMI 3581 GGGACCT [GA]G^G[AT]CC[CT]
4761 GGGACCT
SexAI 110 ACCTGGT A^CC[AT]GGT
1324 ACCTGGT
SspBI 985 TGTACA T^GTACA
4409 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
AvaII BanI BanII BbsI BbvI
BfaI BglII BmyI BpmI BpuAI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeI
NdeII NlaIII NlaIV NspI PshBI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI