##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        AscI        Asp718I     AspEI       AviII       
BbrPI       BcgI        BfrI        BsaBI       BseRI       
BsiEI       BsiWI       BspDI       BssHII      Bst1107I    
BstAPI      BstEII      BstPI       BstZ17I     ClaI        
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco52I      EcoO65I     FseI        FspI        
HgaI        HindIII     HpaI        KpnI        KspI        
MamI        MluI        NheI        NotI        NruI        
PacI        PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PstI        PvuI        
RsrII       SacII       SalI        SbfI        SfiI        
SgrAI       SmaI        SpeI        Sse8387I    SwaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI         2742  GTCTAC               GT^[AC][GT]AC
AccII        4593  CGCG                 CG^CG
Alw44I       1530  GTGCAC               G^TGCAC
Aor51HI      1301  AGCGCT               AGC^GCT
ApaLI        1530  GTGCAC               G^TGCAC
AseI         3410  ATTAAT               AT^TAAT
AsnI         3410  ATTAAT               AT^TAAT
AvrII        2890  CCTAGG               C^CTAGG
BciVI         167  GGATAC               GTATCC(6/5)
BlnI         2890  CCTAGG               C^CTAGG
BlpI          441  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I      441  GCTTAGC              GC^T[ACGT]AGC
BsmI         3985  GAATGC               GAATGC(1/-1)
Bsp1407I     4288  TGTACA               T^GTACA
BspHI        2823  TCATGA               T^CATGA
BspMI        2097  GCAGGT               ACCTGC(4/8)
BsrBI         670  CCGCTC               CCGCTC(-3/-3)
BsrGI        4288  TGTACA               T^GTACA
BstBI         832  TTCGAA               TT^CGAA
BstUI        4593  CGCG                 CG^CG
BstXI         656  CCATGGTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         441  GCTTAGC              GC^T[ACGT]AGC
Eco47III     1301  AGCGCT               AGC^GCT
EcoNI         181  CCTGAGCTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        3315  GAATTC               G^AATTC
EcoRV        1183  GATATC               GAT^ATC
EcoT22I      4059  ATGCAT               ATGCA^T
MfeI          564  CAATTG               C^AATTG
MunI          564  CAATTG               C^AATTG
MvnI         4593  CGCG                 CG^CG
NaeI         1779  GCCGGC               GCC^GGC
NgoMIV       1779  GCCGGC               G^CCGGC
NsiI         4059  ATGCAT               ATGCA^T
NspV          832  TTCGAA               TT^CGAA
PaeR7I       2946  CTCGAG               C^TCGAG
PshBI        3410  ATTAAT               AT^TAAT
RcaI         2823  TCATGA               T^CATGA
SacI         3069  GAGCTC               GAGCT^C
SapI         2212  GAAGAGC              GCTCTTC(1/4)
ScaI         2192  AGTACT               AGT^ACT
SexAI         422  ACCAGGT              A^CC[AT]GGT
SfuI          832  TTCGAA               TT^CGAA
SnaBI        2745  TACGTA               TAC^GTA
SspBI        4288  TGTACA               T^GTACA
XbaI         3961  TCTAGA               T^CTAGA
XhoI         2946  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AcyI         1587  GGCGCC               G[GA]^CG[CT]C
             4739  GGCGCC              
AlwNI         499  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2648  CAGTAACTG           
AocI          162  CCTGAGG              CC^T[ACGT]AGG
             1571  CCTCAGG             
Asp700I      2793  GAAAATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3503  GAACAATTTC          
AspI         2871  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4858  GACTCCGTC           
BalI          906  TGGCCA               TGG^CCA
             1518  TGGCCA              
BamHI        3078  GGATCC               G^GATCC
             3351  GGATCC              
BbeI         1587  GGCGCC               GGCGC^C
             4739  GGCGCC              
BbvCI         379  CCTCAGC              CCTCAGC(-5/-2)
             4767  GCTGAGG             
BclI          582  TGATCA               T^GATCA
              812  TGATCA              
BglI          523  GCCGACAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             4790  GCCTGGGAGGC         
BglII        1271  AGATCT               A^GATCT
             2010  AGATCT              
BsaAI        1579  CACGTA               [CT]AC^GT[GA]
             2745  TACGTA              
BsaHI        1587  GGCGCC               G[GA]^CG[CT]C
             4739  GGCGCC              
BsgI         2016  CTGCAC               GTGCAG(16/14)
             2901  GTGCAG              
BsrFI         281  ACCGGC               [GA]^CCGG[CT]
             1779  GCCGGC              
Bsu36I        162  CCTGAGG              CC^T[ACGT]AGG
             1571  CCTCAGG             
Cfr10I        281  ACCGGC               [GA]^CCGG[CT]
             1779  GCCGGC              
Eco81I        162  CCTGAGG              CC^T[ACGT]AGG
             1571  CCTCAGG             
FbaI          582  TGATCA               T^GATCA
              812  TGATCA              
Hin1I        1587  GGCGCC               G[GA]^CG[CT]C
             4739  GGCGCC              
HincII       3333  GTTGAC               GT[CT]^[GA]AC
             3766  GTCAAC              
HindII       3333  GTTGAC               GT[CT]^[GA]AC
             3766  GTCAAC              
KasI         1587  GGCGCC               G^GCGCC
             4739  GGCGCC              
MluNI         906  TGGCCA               TGG^CCA
             1518  TGGCCA              
MscI          906  TGGCCA               TGG^CCA
             1518  TGGCCA              
NarI         1587  GGCGCC               GG^CGCC
             4739  GGCGCC              
PflFI        2871  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4858  GACTCCGTC           
PvuII        1535  CAGCTG               CAG^CTG
             2321  CAGCTG              
SfoI         1587  GGCGCC               GGC^GCC
             4739  GGCGCC              
SphI          574  GCATGC               GCATG^C
             2999  GCATGC              
Tth111I      2871  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4858  GACTCCGTC           
XmnI         2793  GAAAATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3503  GAACAATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AccIII      AciI        AcsI        AfaI        AflIII      
AluI        AlwI        ApaI        ApoI        AspHI       
AvaI        AvaII       BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaI        BsaJI       BsaWI       BseAI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    BspEI       BspLU11I    
BsrDI       BsrI        BssKI       BssSI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MroI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII