##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Alw44I ApaLI AscI AspEI AviII
BbrPI BclI BfrI BsaAI BsiWI
Bsp1407I BspDI BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
DraI DrdI Eam1105I EcoRI EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KspI MfeI MluI
MunI NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfuI SnaBI
SpeI Sse8387I SspBI SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 1298 GGTACC G^GTACC
AccI 2928 GTAGAC GT^[AC][GT]AC
AccIII 88 TCCGGA T^CCGGA
AcsI 288 AAATTT [GA]^AATT[CT]
AcyI 3432 GGCGCC G[GA]^CG[CT]C
ApoI 288 AAATTT [GA]^AATT[CT]
AseI 3750 ATTAAT AT^TAAT
AsnI 3750 ATTAAT AT^TAAT
Asp700I 1311 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1298 GGTACC G^GTACC
AspI 3081 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4408 CCTAGG C^CTAGG
BalI 263 TGGCCA TGG^CCA
BbeI 3432 GGCGCC GGCGC^C
BcgI 436 GCAAAGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2269 AGATCT A^GATCT
BlnI 4408 CCTAGG C^CTAGG
BlpI 699 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 699 GCTGAGC GC^T[ACGT]AGC
BsaHI 3432 GGCGCC G[GA]^CG[CT]C
BsaI 2186 GGTCTC GGTCTC(1/5)
BseAI 88 TCCGGA T^CCGGA
BspEI 88 TCCGGA T^CCGGA
BspHI 4768 TCATGA T^CATGA
BspLU11I 316 ACATGT A^CATGT
BssSI 2922 CACGAG CACGAG(-5/-1)
BstEII 2663 GGTGACC G^GT[ACGT]ACC
BstPI 2663 GGTGACC G^GT[ACGT]ACC
CelII 699 GCTGAGC GC^T[ACGT]AGC
EagI 1425 CGGCCG C^GGCCG
EclXI 1425 CGGCCG C^GGCCG
Eco52I 1425 CGGCCG C^GGCCG
EcoO65I 2663 GGTGACC G^GT[ACGT]ACC
EcoRV 4322 GATATC GAT^ATC
HgaI 412 GCGTC GACGC(5/10)
Hin1I 3432 GGCGCC G[GA]^CG[CT]C
KasI 3432 GGCGCC G^GCGCC
KpnI 1298 GGTACC GGTAC^C
MluNI 263 TGGCCA TGG^CCA
MroI 88 TCCGGA T^CCGGA
MscI 263 TGGCCA TGG^CCA
NarI 3432 GGCGCC GG^CGCC
NdeI 5016 CATATG CA^TATG
PflFI 3081 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2871 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3750 ATTAAT AT^TAAT
RcaI 4768 TCATGA T^CATGA
SapI 512 GCTCTTC GCTCTTC(1/4)
SfoI 3432 GGCGCC GGC^GCC
SgrAI 1200 CACCGGCG C[GA]^CCGG[CT]G
Tth111I 3081 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1311 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 199 CGCG CG^CG
2115 CGCG
AflIII 316 ACATGT A^C[GA][CT]GT
688 ACACGT
Aor51HI 457 AGCGCT AGC^GCT
553 AGCGCT
BbsI 1311 GAAGAC GAAGAC(2/6)
4800 GAAGAC
BciVI 3643 GGATAC GTATCC(6/5)
613 GTATCC
BpuAI 1311 GAAGAC GAAGAC(2/6)
4800 GAAGAC
BsaBI 1395 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3290 GATTGAAATC
BsiEI 1425 CGGCCG CG[GA][CT]^CG
3313 CGACCG
BsmBI 1174 CGTCTC CGTCTC(1/5)
4847 GAGACG
BstUI 199 CGCG CG^CG
2115 CGCG
BstXI 1118 CCAGCCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4836 CCACCCACCTGG
Eco47III 457 AGCGCT AGC^GCT
553 AGCGCT
MamI 1395 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3290 GATTGAAATC
MvnI 199 CGCG CG^CG
2115 CGCG
NaeI 1150 GCCGGC GCC^GGC
1448 GCCGGC
NgoMIV 1150 GCCGGC G^CCGGC
1448 GCCGGC
ScaI 1363 AGTACT AGT^ACT
1405 AGTACT
SfiI 982 GGCCTGAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2855 GGCCAGCCCGGCC
XcmI 370 CCAACCACAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4737 CCAGACGAGGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BamHI BanI BanII BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PaeR7I PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoI XhoII XmaCI
XmaI