##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
AhdI AocI Aor51HI ApaI AscI
Asp718I AspEI AspI AvaI AviII
BamHI BbrPI BcgI BlpI Bpu1102I
BsiEI BsiWI BsmI BsoBI BspLU11I
BspMI BsrFI BssHII BssSI BstEII
BstPI Bsu36I CelII Cfr10I CpoI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I FseI FspI KpnI
KspI MluI NaeI NgoMIV NheI
NotI NruI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SgrAI SmaI SnaBI
Sse8387I SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccII 174 CGCG CG^CG
BalI 2095 TGGCCA TGG^CCA
BglI 4719 GCCTTGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1012 AGATCT A^GATCT
BsaI 1844 GGTCTC GGTCTC(1/5)
BseRI 3348 GAGGAG GAGGAG(10/8)
BsmBI 154 CGTCTC CGTCTC(1/5)
BspDI 1763 ATCGAT AT^CGAT
BsrBI 142 GAGCGG CCGCTC(-3/-3)
Bst1107I 4681 GTATAC GTA^TAC
BstBI 1664 TTCGAA TT^CGAA
BstUI 174 CGCG CG^CG
BstXI 781 CCAGATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4681 GTATAC GTA^TAC
ClaI 1763 ATCGAT AT^CGAT
DraIII 3419 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 349 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1736 ATGCAT ATGCA^T
HgaI 511 GCGTC GACGC(5/10)
HindIII 316 AAGCTT A^AGCTT
HpaI 2219 GTTAAC GTT^AAC
MfeI 4075 CAATTG C^AATTG
MluNI 2095 TGGCCA TGG^CCA
MscI 2095 TGGCCA TGG^CCA
MunI 4075 CAATTG C^AATTG
MvnI 174 CGCG CG^CG
NsiI 1736 ATGCAT ATGCA^T
NspV 1664 TTCGAA TT^CGAA
PacI 2495 TTAATTAA TTAAT^TAA
PpuMI 2701 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 944 GAGCTC GAGCT^C
SapI 2627 GCTCTTC GCTCTTC(1/4)
ScaI 4730 AGTACT AGT^ACT
SfuI 1664 TTCGAA TT^CGAA
StuI 4717 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccI 3062 GTAGAC GT^[AC][GT]AC
4681 GTATAC
AccIII 311 TCCGGA T^CCGGA
1302 TCCGGA
AcyI 31 GGCGCC G[GA]^CG[CT]C
1880 GGCGCC
AflII 184 CTTAAG C^TTAAG
4635 CTTAAG
Alw44I 1670 GTGCAC G^TGCAC
4614 GTGCAC
ApaLI 1670 GTGCAC G^TGCAC
4614 GTGCAC
AvrII 1486 CCTAGG C^CTAGG
4271 CCTAGG
BanII 944 GAGCTC G[GA]GC[CT]^C
2599 GGGCTC
BbeI 31 GGCGCC GGCGC^C
1880 GGCGCC
BciVI 1512 GGATAC GTATCC(6/5)
1296 GTATCC
BfrI 184 CTTAAG C^TTAAG
4635 CTTAAG
BlnI 1486 CCTAGG C^CTAGG
4271 CCTAGG
BpmI 802 CTCCAG CTGGAG(16/14)
302 CTGGAG
BsaAI 244 CACGTA [CT]AC^GT[GA]
3165 TACGTG
BsaHI 31 GGCGCC G[GA]^CG[CT]C
1880 GGCGCC
BsaWI 311 TCCGGA [AT]^CCGG[AT]
1302 TCCGGA
BseAI 311 TCCGGA T^CCGGA
1302 TCCGGA
Bsp1407I 69 TGTACA T^GTACA
3032 TGTACA
BspEI 311 TCCGGA T^CCGGA
1302 TCCGGA
BspHI 471 TCATGA T^CATGA
1089 TCATGA
BsrGI 69 TGTACA T^GTACA
3032 TGTACA
BstAPI 1432 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1456 GCAGTGTCTGC
DraII 1130 GGGGCCT [GA]G^G[ACGT]CC[CT]
2701 AGGTCCT
DrdI 97 GACTCCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2371 GACTGATTTGTC
EaeI 2095 TGGCCA [CT]^GGCC[GA]
3561 CGGCCA
EarI 2628 CTCTTC CTCTTC(1/4)
2726 GAAGAG
EcoO109I 1130 GGGGCCT [GA]G^G[ACGT]CC[CT]
2701 AGGTCCT
FauI 197 CCCGC CCCGC(4/6)
28 GCGGG
HaeII 31 GGCGCC [GA]GCGC^[CT]
1880 GGCGCC
Hin1I 31 GGCGCC G[GA]^CG[CT]C
1880 GGCGCC
KasI 31 GGCGCC G^GCGCC
1880 GGCGCC
Ksp632I 2628 CTCTTC CTCTTC(1/4)
2726 GAAGAG
MroI 311 TCCGGA T^CCGGA
1302 TCCGGA
NarI 31 GGCGCC GG^CGCC
1880 GGCGCC
PflMI 2111 CCAGCGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2888 CCACTTACTGG
RcaI 471 TCATGA T^CATGA
1089 TCATGA
SfoI 31 GGCGCC GGC^GCC
1880 GGCGCC
SpeI 2443 ACTAGT A^CTAGT
3392 ACTAGT
SphI 1791 GCATGC GCATG^C
4232 GCATGC
SspBI 69 TGTACA T^GTACA
3032 TGTACA
Van91I 2111 CCAGCGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2888 CCACTTACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AspHI AvaII BanI BbsI BbvCI
BbvI BclI BcnI BfaI BmyI
BpuAI BsaBI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DsaI EcoRI EcoRII
EcoRV EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeI NdeII NlaIII NlaIV
NspI PleI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI