##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

Acc65I      AclI        AflII       AgeI        AhdI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       BbrPI       BciVI       
BfrI        BlpI        Bpu1102I    BsaAI       BsiWI       
BsmBI       BsmI        BspDI       BspHI       BspLU11I    
BsrBI       BssHII      BssSI       Bst1107I    BstBI       
BstZ17I     CelII       ClaI        CpoI        DraIII      
DrdI        Eam1105I    EcoRV       EcoT22I     FspI        
HpaI        KpnI        MfeI        MluI        MunI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SalI        SbfI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        Tth111I     XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AatII        1437  GACGTC               GACGT^C
AccI         2631  GTAGAC               GT^[AC][GT]AC
AccIII       2881  TCCGGA               T^CCGGA
AflIII       2638  ACGTGT               A^C[GA][CT]GT
Aor51HI       925  AGCGCT               AGC^GCT
AvrII        3765  CCTAGG               C^CTAGG
BamHI        3839  GGATCC               G^GATCC
BbeI         1669  GGCGCC               GGCGC^C
BbvCI        2935  CCTCAGC              CCTCAGC(-5/-2)
BglII        1018  AGATCT               A^GATCT
BlnI         3765  CCTAGG               C^CTAGG
BsaBI        3208  GATTGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2881  TCCGGA               [AT]^CCGG[AT]
BseAI        2881  TCCGGA               T^CCGGA
BsiEI        4729  CGGCCG               CG[GA][CT]^CG
BspEI        2881  TCCGGA               T^CCGGA
BsrFI        4545  GCCGGC               [GA]^CCGG[CT]
BstEII       3096  GGTGACC              G^GT[ACGT]ACC
BstPI        3096  GGTGACC              G^GT[ACGT]ACC
Cfr10I       4545  GCCGGC               [GA]^CCGG[CT]
EagI         4729  CGGCCG               C^GGCCG
EclXI        4729  CGGCCG               C^GGCCG
Eco47III      925  AGCGCT               AGC^GCT
Eco52I       4729  CGGCCG               C^GGCCG
EcoO65I      3096  GGTGACC              G^GT[ACGT]ACC
EcoRI        2906  GAATTC               G^AATTC
FseI         4544  GGCCGGCC             GGCCGG^CC
KasI         1669  GGCGCC               G^GCGCC
KspI         1835  CCGCGG               CCGC^GG
MamI         3208  GATTGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2881  TCCGGA               T^CCGGA
NaeI         4545  GCCGGC               GCC^GGC
NarI         1669  GGCGCC               GG^CGCC
NdeI         1228  CATATG               CA^TATG
NgoMIV       4545  GCCGGC               G^CCGGC
SacI         3171  GAGCTC               GAGCT^C
SacII        1835  CCGCGG               CCGC^GG
SexAI        4531  ACCTGGT              A^CC[AT]GGT
SfiI         4721  GGCCCTCACGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1669  GGCGCC               GGC^GCC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AcsI         1142  GAATTT               [GA]^AATT[CT]
             2906  GAATTC              
ApoI         1142  GAATTT               [GA]^AATT[CT]
             2906  GAATTC              
BclI          335  TGATCA               T^GATCA
             4471  TGATCA              
Bsp1407I     3222  TGTACA               T^GTACA
             4627  TGTACA              
BsrDI        1283  CATTGC               GCAATG(2/0)
             3864  CATTGC              
BsrGI        3222  TGTACA               T^GTACA
             4627  TGTACA              
DraI         1025  TTTAAA               TTT^AAA
             1193  TTTAAA              
FbaI          335  TGATCA               T^GATCA
             4471  TGATCA              
HincII       1521  GTTGAC               GT[CT]^[GA]AC
             4786  GTCAAC              
HindII       1521  GTTGAC               GT[CT]^[GA]AC
             4786  GTCAAC              
PaeR7I       1856  CTCGAG               C^TCGAG
             2851  CTCGAG              
PpuMI           5  AGGTCCT              [GA]G^G[AT]CC[CT]
              844  AGGACCC             
ScaI          703  AGTACT               AGT^ACT
             4249  AGTACT              
SspBI        3222  TGTACA               T^GTACA
             4627  TGTACA              
XhoI         1856  CTCGAG               C^TCGAG
             2851  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  128 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      AlwI        AlwNI       AocI        ApaI        
ApaLI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvI        BcgI        
BcnI        BfaI        BglI        BmyI        BpmI        
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PstI        PvuII       RsaI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        SmlI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI