##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI Asp718I AspEI AspI
AvrII BamHI BbeI BbrPI BcgI
BglI BlnI BsaWI BseAI BsiWI
BsmBI BspDI BspEI BspHI BsrDI
BssSI Bst1107I BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DrdI Eam1105I
Eco47III Eco81I EcoO65I FseI HpaI
KasI KpnI MluI MroI NarI
NcoI NheI NotI NruI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SpeI Sse8387I Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcyI 298 GACGCC G[GA]^CG[CT]C
AflII 2315 CTTAAG C^TTAAG
AspHI 2645 GAGCTC G[AT]GC[AT]^C
AviII 3912 TGCGCA TGC^GCA
BalI 959 TGGCCA TGG^CCA
BbvCI 176 GCTGAGG CCTCAGC(-5/-2)
BciVI 1670 GGATAC GTATCC(6/5)
BfrI 2315 CTTAAG C^TTAAG
BsaAI 2722 TACGTA [CT]AC^GT[GA]
BsaBI 4444 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 298 GACGCC G[GA]^CG[CT]C
BsgI 898 GTGCAG GTGCAG(16/14)
BsiEI 189 CGGCCG CG[GA][CT]^CG
BsiHKAI 2645 GAGCTC G[AT]GC[AT]^C
Bsp1407I 699 TGTACA T^GTACA
BsrGI 699 TGTACA T^GTACA
BssHII 69 GCGCGC G^CGCGC
BstBI 3641 TTCGAA TT^CGAA
DraIII 1202 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 189 CGGCCG C^GGCCG
EarI 4333 GAAGAG CTCTTC(1/4)
EclXI 189 CGGCCG C^GGCCG
Eco52I 189 CGGCCG C^GGCCG
EcoNI 2023 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2419 GATATC GAT^ATC
FspI 3912 TGCGCA TGC^GCA
Hin1I 298 GACGCC G[GA]^CG[CT]C
HincII 677 GTCAAC GT[CT]^[GA]AC
HindII 677 GTCAAC GT[CT]^[GA]AC
Ksp632I 4333 GAAGAG CTCTTC(1/4)
MamI 4444 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 959 TGGCCA TGG^CCA
MscI 959 TGGCCA TGG^CCA
NspV 3641 TTCGAA TT^CGAA
PacI 3225 TTAATTAA TTAAT^TAA
PstI 3575 CTGCAG CTGCA^G
SacI 2645 GAGCTC GAGCT^C
SfuI 3641 TTCGAA TT^CGAA
SmaI 508 CCCGGG CCC^GGG
SnaBI 2722 TACGTA TAC^GTA
SphI 1743 GCATGC GCATG^C
SspBI 699 TGTACA T^GTACA
StuI 1077 AGGCCT AGG^CCT
SwaI 4445 ATTTAAAT ATTT^AAAT
XmaCI 508 CCCGGG C^CCGGG
XmaI 508 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 736 ACATGT A^C[GA][CT]GT
751 ACATGT
ApaI 95 GGGCCC GGGCC^C
194 GGGCCC
BanI 552 GGTGCC G^G[CT][GA]CC
3020 GGTGCC
BsaI 2088 GAGACC GGTCTC(1/5)
4413 GAGACC
BspLU11I 736 ACATGT A^CATGT
751 ACATGT
BsrBI 327 CCGCTC CCGCTC(-3/-3)
46 GAGCGG
BstAPI 400 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1584 GCAAACAATGC
BstXI 532 CCAGCAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1922 CCATACAGTTGG
DraI 2810 TTTAAA TTT^AAA
4446 TTTAAA
EcoRI 2884 GAATTC G^AATTC
3991 GAATTC
EcoT14I 2950 CCTTGG C^C[AT][AT]GG
3024 CCAAGG
EcoT22I 3381 ATGCAT ATGCA^T
3536 ATGCAT
HaeII 173 GGCGCT [GA]GCGC^[CT]
486 AGCGCC
HgaI 298 GACGC GACGC(5/10)
1185 GCGTC
HindIII 809 AAGCTT A^AGCTT
4075 AAGCTT
KspI 129 CCGCGG CCGC^GG
216 CCGCGG
NdeI 3284 CATATG CA^TATG
3409 CATATG
NsiI 3381 ATGCAT ATGCA^T
3536 ATGCAT
PvuII 2124 CAGCTG CAG^CTG
2458 CAGCTG
SacII 129 CCGCGG CCGC^GG
216 CCGCGG
StyI 2950 CCTTGG C^C[AT][AT]GG
3024 CCAAGG
Tsp45I 1988 GTCAC ^GT[GC]AC
2593 GTCAC
XbaI 2375 TCTAGA T^CTAGA
3519 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AvaI AvaII BanII BbsI
BbvI BclI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspA1I MspI MunI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PshBI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI