##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AclI AhdI AocI
Aor51HI AscI Asp718I AspEI AviII
BbeI BbvCI BcgI BclI BglII
BsaBI BsiWI Bsp1407I BspDI BsrBI
BsrGI BssHII BssSI BstBI BstEII
BstPI Bsu36I ClaI CpoI DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HpaI KasI KpnI KspI MamI
MluI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PmeI PpuMI PshAI
Psp1406I PvuI RsrII SacII SapI
SbfI ScaI SfoI SfuI SgrAI
SpeI SphI Sse8387I SspBI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AflII 4060 CTTAAG C^TTAAG
AgeI 1346 ACCGGT A^CCGGT
Alw44I 2848 GTGCAC G^TGCAC
ApaI 3046 GGGCCC GGGCC^C
ApaLI 2848 GTGCAC G^TGCAC
AseI 3895 ATTAAT AT^TAAT
AsnI 3895 ATTAAT AT^TAAT
BciVI 4462 GGATAC GTATCC(6/5)
BfrI 4060 CTTAAG C^TTAAG
BlpI 3679 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3679 GCTGAGC GC^T[ACGT]AGC
BsaI 4727 GAGACC GGTCTC(1/5)
BsiEI 1975 CGGCCG CG[GA][CT]^CG
BspLU11I 2280 ACATGT A^CATGT
Bst1107I 4655 GTATAC GTA^TAC
BstXI 1818 CCAAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4655 GTATAC GTA^TAC
CelII 3679 GCTGAGC GC^T[ACGT]AGC
DraIII 2601 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2661 CCACGG C^C[GA][CT]GG
EagI 1975 CGGCCG C^GGCCG
EclXI 1975 CGGCCG C^GGCCG
Eco52I 1975 CGGCCG C^GGCCG
EcoRV 1286 GATATC GAT^ATC
HaeII 2397 AGCGCC [GA]GCGC^[CT]
HindIII 283 AAGCTT A^AGCTT
MfeI 4773 CAATTG C^AATTG
MunI 4773 CAATTG C^AATTG
NdeI 923 CATATG CA^TATG
PflMI 2600 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1346 ACCGGT A^CCGGT
PshBI 3895 ATTAAT AT^TAAT
SacI 2123 GAGCTC GAGCT^C
SalI 3464 GTCGAC G^TCGAC
SfiI 2638 GGCCGGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 972 CCCGGG CCC^GGG
SnaBI 725 TACGTA TAC^GTA
SspI 4821 AATATT AAT^ATT
Van91I 2600 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2722 CCAGGGGATTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 972 CCCGGG C^CCGGG
XmaI 972 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 24 CGCG CG^CG
74 CGCG
AccIII 2443 TCCGGA T^CCGGA
2803 TCCGGA
Asp700I 3727 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3918 GAACATTTTC
AspI 3093 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4099 GACCTGGTC
AvrII 41 CCTAGG C^CTAGG
3997 CCTAGG
BamHI 454 GGATCC G^GATCC
2440 GGATCC
BlnI 41 CCTAGG C^CTAGG
3997 CCTAGG
BpmI 3419 CTCCAG CTGGAG(16/14)
4722 CTGGAG
BseAI 2443 TCCGGA T^CCGGA
2803 TCCGGA
BsmBI 3006 CGTCTC CGTCTC(1/5)
102 GAGACG
BspEI 2443 TCCGGA T^CCGGA
2803 TCCGGA
BspHI 237 TCATGA T^CATGA
2908 TCATGA
BstUI 24 CGCG CG^CG
74 CGCG
MroI 2443 TCCGGA T^CCGGA
2803 TCCGGA
MvnI 24 CGCG CG^CG
74 CGCG
PflFI 3093 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4099 GACCTGGTC
RcaI 237 TCATGA T^CATGA
2908 TCATGA
SexAI 3117 ACCTGGT A^CC[AT]GGT
4100 ACCTGGT
StuI 272 AGGCCT AGG^CCT
3415 AGGCCT
Tth111I 3093 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4099 GACCTGGTC
XmnI 3727 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3918 GAACATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbrPI BbsI BbvI BcnI
BfaI BglI BmyI BpuAI BsaAI
BsaHI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PmaCI PmlI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII