##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BciVI BclI BfrI
BlnI BsiWI BsmI BspDI BspHI
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI EcoRI EcoRV FbaI FseI
FspI HincII HindII HpaI KpnI
MfeI MluI MunI NdeI NheI
NotI NruI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I RcaI
RsrII SacI SalI ScaI SfuI
SpeI SphI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 1272 GACGTC GACGT^C
AcsI 5402 AAATTT [GA]^AATT[CT]
AocI 3435 CCTGAGG CC^T[ACGT]AGG
ApoI 5402 AAATTT [GA]^AATT[CT]
BbrPI 2670 CACGTG CAC^GTG
BbvCI 3905 GCTGAGG CCTCAGC(-5/-2)
BglII 349 AGATCT A^GATCT
BsaBI 350 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 5383 TGTACA T^GTACA
BspLU11I 1150 ACATGT A^CATGT
BsrGI 5383 TGTACA T^GTACA
BstEII 1119 GGTGACC G^GT[ACGT]ACC
BstPI 1119 GGTGACC G^GT[ACGT]ACC
Bsu36I 3435 CCTGAGG CC^T[ACGT]AGG
DraI 5399 TTTAAA TTT^AAA
EagI 4055 CGGCCG C^GGCCG
EclXI 4055 CGGCCG C^GGCCG
Eco52I 4055 CGGCCG C^GGCCG
Eco81I 3435 CCTGAGG CC^T[ACGT]AGG
EcoNI 1478 CCTCGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1119 GGTGACC G^GT[ACGT]ACC
HindIII 1814 AAGCTT A^AGCTT
KspI 5100 CCGCGG CCGC^GG
MamI 350 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PaeR7I 912 CTCGAG C^TCGAG
PmaCI 2670 CACGTG CAC^GTG
PmlI 2670 CACGTG CAC^GTG
PvuI 36 CGATCG CGAT^CG
SacII 5100 CCGCGG CCGC^GG
SnaBI 2274 TACGTA TAC^GTA
SspBI 5383 TGTACA T^GTACA
SspI 5395 AATATT AAT^ATT
SwaI 5398 ATTTAAAT ATTT^AAAT
Tsp509I 5403 AATT ^AATT
XhoI 912 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 2365 TCCGGA T^CCGGA
2993 TCCGGA
AhdI 1755 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4826 GACATCACGTC
Aor51HI 406 AGCGCT AGC^GCT
871 AGCGCT
AspEI 1755 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4826 GACATCACGTC
AspI 480 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4343 GACACTGTC
BamHI 2851 GGATCC G^GATCC
3153 GGATCC
BcgI 4660 CGACCTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1471 GCAAGATCCTCG
BlpI 188 GCTCAGC GC^T[ACGT]AGC
1073 GCTGAGC
Bpu1102I 188 GCTCAGC GC^T[ACGT]AGC
1073 GCTGAGC
BsaAI 2274 TACGTA [CT]AC^GT[GA]
2670 CACGTG
BsaWI 2365 TCCGGA [AT]^CCGG[AT]
2993 TCCGGA
BseAI 2365 TCCGGA T^CCGGA
2993 TCCGGA
BsiEI 36 CGATCG CG[GA][CT]^CG
4055 CGGCCG
BsmBI 1700 CGTCTC CGTCTC(1/5)
4768 GAGACG
BspEI 2365 TCCGGA T^CCGGA
2993 TCCGGA
BsrDI 4930 CATTGC GCAATG(2/0)
1792 GCAATG
BssHII 53 GCGCGC G^CGCGC
507 GCGCGC
CelII 188 GCTCAGC GC^T[ACGT]AGC
1073 GCTGAGC
DraIII 1044 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
3670 CACCGAGTG
Eam1105I 1755 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4826 GACATCACGTC
Eco47III 406 AGCGCT AGC^GCT
871 AGCGCT
EcoT22I 1594 ATGCAT ATGCA^T
3134 ATGCAT
MroI 2365 TCCGGA T^CCGGA
2993 TCCGGA
MseI 5400 TTAA T^TAA
5431 TTAA
NcoI 160 CCATGG C^CATGG
1235 CCATGG
NsiI 1594 ATGCAT ATGCA^T
3134 ATGCAT
PflFI 480 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4343 GACACTGTC
PflMI 3920 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4717 CCACAGCCTGG
SfiI 2757 GGCCTAAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4942 GGCCCGCCAGGCC
SgrAI 3161 CGCCGGCG C[GA]^CCGG[CT]G
4075 CGCCGGCG
Tru9I 5400 TTAA T^TAA
5431 TTAA
Tth111I 480 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4343 GACACTGTC
Van91I 3920 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4717 CCACAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
SbfI ScrFI SexAI SfaNI SfcI
SfoI SmaI SmlI Sse8387I StuI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI