##################################
# NO CUT ENZYMES (   42 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        AscI        
AseI        AsnI        Asp718I     AviII       BbrPI       
BsiWI       BspLU11I    Bst1107I    BstEII      BstPI       
BstZ17I     DraIII      EcoO65I     EcoRI       FspI        
HpaI        KpnI        MfeI        MluI        MunI        
NdeI        NotI        NruI        PacI        PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        SalI        SapI        SgrAI       SnaBI       
SspI        SwaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccI         1939  GTCTAC               GT^[AC][GT]AC
AccIII       2310  TCCGGA               T^CCGGA
AflIII        398  ACGTGT               A^C[GA][CT]GT
AhdI          574  GACACCCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2717  GTGCAC               G^TGCAC
ApaLI        2717  GTGCAC               G^TGCAC
Asp700I      4949  GAAATCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         574  GACACCCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          491  TGGCCA               TGG^CCA
BamHI         258  GGATCC               G^GATCC
BcgI         2430  GCAAGCTTCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         5102  TGATCA               T^GATCA
BglII        3634  AGATCT               A^GATCT
BseAI        2310  TCCGGA               T^CCGGA
BsmBI        2842  CGTCTC               CGTCTC(1/5)
Bsp1407I     3222  TGTACA               T^GTACA
BspDI         386  ATCGAT               AT^CGAT
BspEI        2310  TCCGGA               T^CCGGA
BsrBI        4477  GAGCGG               CCGCTC(-3/-3)
BsrDI         405  CATTGC               GCAATG(2/0)
BsrGI        3222  TGTACA               T^GTACA
BssSI        1288  CACGAG               CACGAG(-5/-1)
BstAPI       4649  GCAAATTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         671  TTCGAA               TT^CGAA
ClaI          386  ATCGAT               AT^CGAT
CpoI         2195  CGGACCG              CG^G[AT]CCG
Eam1105I      574  GACACCCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV        2534  GATATC               GAT^ATC
FbaI         5102  TGATCA               T^GATCA
FseI           31  GGCCGGCC             GGCCGG^CC
HincII        238  GTTGAC               GT[CT]^[GA]AC
HindII        238  GTTGAC               GT[CT]^[GA]AC
MluNI         491  TGGCCA               TGG^CCA
MroI         2310  TCCGGA               T^CCGGA
MscI          491  TGGCCA               TGG^CCA
NcoI         3740  CCATGG               C^CATGG
NheI         4645  GCTAGC               G^CTAGC
NspV          671  TTCGAA               TT^CGAA
PshAI         373  GACGTGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII        2195  CGGACCG              CG^G[AT]CCG
ScaI         1176  AGTACT               AGT^ACT
SexAI        1046  ACCTGGT              A^CC[AT]GGT
SfiI         3932  GGCCTCCTGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI          671  TTCGAA               TT^CGAA
SmaI           60  CCCGGG               CCC^GGG
SpeI         3926  ACTAGT               A^CTAGT
SphI         2867  GCATGC               GCATG^C
SspBI        3222  TGTACA               T^GTACA
XbaI         1605  TCTAGA               T^CTAGA
XmaCI          60  CCCGGG               C^CCGGG
XmaI           60  CCCGGG               C^CCGGG
XmnI         4949  GAAATCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AflII        2412  CTTAAG               C^TTAAG
             4981  CTTAAG              
AlwNI        1060  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2006  CAGTGCCTG           
Aor51HI      1093  AGCGCT               AGC^GCT
             1361  AGCGCT              
AspI         1723  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2837  GACCTCGTC           
AvrII        2820  CCTAGG               C^CTAGG
             4104  CCTAGG              
BciVI        1495  GTATCC               GTATCC(6/5)
             4242  GTATCC              
BfrI         2412  CTTAAG               C^TTAAG
             4981  CTTAAG              
BglI         2794  GCCATGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3933  GCCTCCTGGGC         
BlnI         2820  CCTAGG               C^CTAGG
             4104  CCTAGG              
BsaAI         397  TACGTG               [CT]AC^GT[GA]
             2471  CACGTA              
BsaBI        1611  GATTCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2233  GATGAGGATC          
BsaWI         590  ACCGGA               [AT]^CCGG[AT]
             2310  TCCGGA              
BsiEI          30  CGGCCG               CG[GA][CT]^CG
               73  CGGCCG              
BspHI        1385  TCATGA               T^CATGA
             1733  TCATGA              
DrdI         1249  GACGGGGCGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1678  GACATCTCTGTC        
EagI           30  CGGCCG               C^GGCCG
               73  CGGCCG              
EclXI          30  CGGCCG               C^GGCCG
               73  CGGCCG              
Eco47III     1093  AGCGCT               AGC^GCT
             1361  AGCGCT              
Eco52I         30  CGGCCG               C^GGCCG
               73  CGGCCG              
EcoT22I      3258  ATGCAT               ATGCA^T
             4192  ATGCAT              
HindIII      2432  AAGCTT               A^AGCTT
             3657  AAGCTT              
MamI         1611  GATTCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2233  GATGAGGATC          
NaeI           32  GCCGGC               GCC^GGC
              151  GCCGGC              
NgoMIV         32  GCCGGC               G^CCGGC
              151  GCCGGC              
NsiI         3258  ATGCAT               ATGCA^T
             4192  ATGCAT              
PflFI        1723  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2837  GACCTCGTC           
RcaI         1385  TCATGA               T^CATGA
             1733  TCATGA              
SacI         2137  GAGCTC               GAGCT^C
             4904  GAGCTC              
Tth111I      1723  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2837  GACCTCGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  135 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AocI        ApaI        ApoI        
AspHI       AvaI        AvaII       BanI        BanII       
BbeI        BbsI        BbvCI       BbvI        BcnI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspMI       
BsrFI       BsrI        BssHII      BssKI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NdeII       NlaIII      NlaIV       NspI        PaeR7I      
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SacII       Sau3AI      Sau96I      SbfI        
ScrFI       SfaNI       SfcI        SfoI        SmlI        
Sse8387I    StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoI        XhoII