##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AhdI Alw44I ApaLI AscI Asp700I
AspEI AviII BamHI BbrPI BclI
BfrI BglII BsaAI BsaBI BsiEI
BsiWI BspDI BspLU11I BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HpaI KspI MamI
MluI NdeI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 3649 GGTACC G^GTACC
AccII 1809 CGCG CG^CG
AccIII 3928 TCCGGA T^CCGGA
AcsI 3186 GAATTC [GA]^AATT[CT]
Aor51HI 2786 AGCGCT AGC^GCT
ApoI 3186 GAATTC [GA]^AATT[CT]
AseI 2756 ATTAAT AT^TAAT
AsnI 2756 ATTAAT AT^TAAT
Asp718I 3649 GGTACC G^GTACC
AvrII 1406 CCTAGG C^CTAGG
BbeI 2357 GGCGCC GGCGC^C
BcgI 4509 CGACACTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2452 GGATAC GTATCC(6/5)
BlnI 1406 CCTAGG C^CTAGG
BseAI 3928 TCCGGA T^CCGGA
Bsp1407I 1190 TGTACA T^GTACA
BspEI 3928 TCCGGA T^CCGGA
BsrDI 2623 CATTGC GCAATG(2/0)
BsrFI 4492 GCCGGC [GA]^CCGG[CT]
BsrGI 1190 TGTACA T^GTACA
BssSI 4002 CACGAG CACGAG(-5/-1)
BstUI 1809 CGCG CG^CG
Cfr10I 4492 GCCGGC [GA]^CCGG[CT]
DraIII 2738 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2786 AGCGCT AGC^GCT
EcoRI 3186 GAATTC G^AATTC
KasI 2357 GGCGCC G^GCGCC
KpnI 3649 GGTACC GGTAC^C
MaeII 1227 ACGT A^CGT
MfeI 4626 CAATTG C^AATTG
MroI 3928 TCCGGA T^CCGGA
MunI 4626 CAATTG C^AATTG
MvnI 1809 CGCG CG^CG
NaeI 4492 GCCGGC GCC^GGC
NarI 2357 GGCGCC GG^CGCC
NgoMIV 4492 GCCGGC G^CCGGC
NspI 2408 GCATGT [GA]CATG^[CT]
PshAI 1805 GACCCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2756 ATTAAT AT^TAAT
SfiI 40 GGCCACCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2357 GGCGCC GGC^GCC
SspBI 1190 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 398 GTCTAC GT^[AC][GT]AC
1157 GTCTAC
AspI 464 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
476 GACTATGTC
BbvCI 177 CCTCAGC CCTCAGC(-5/-2)
2005 CCTCAGC
BlpI 931 GCTGAGC GC^T[ACGT]AGC
3794 GCTGAGC
Bpu1102I 931 GCTGAGC GC^T[ACGT]AGC
3794 GCTGAGC
BsaWI 326 ACCGGA [AT]^CCGG[AT]
3928 TCCGGA
BsmI 1064 GAATGC GAATGC(1/-1)
3770 GAATGC
BspHI 774 TCATGA T^CATGA
1694 TCATGA
BsrBI 1738 CCGCTC CCGCTC(-3/-3)
864 GAGCGG
BstYI 2314 GGATCT [GA]^GATC[CT]
4319 GGATCT
CelII 931 GCTGAGC GC^T[ACGT]AGC
3794 GCTGAGC
EcoNI 1743 CCTTGGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2543 CCTTGGGAAGG
HaeII 2357 GGCGCC [GA]GCGC^[CT]
2786 AGCGCT
HindIII 3476 AAGCTT A^AGCTT
3501 AAGCTT
MflI 2314 GGATCT [GA]^GATC[CT]
4319 GGATCT
MseI 2757 TTAA T^TAA
3780 TTAA
NcoI 615 CCATGG C^CATGG
1200 CCATGG
NheI 2474 GCTAGC G^CTAGC
2864 GCTAGC
PflFI 464 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
476 GACTATGTC
PflMI 1136 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2679 CCAAGAACTGG
RcaI 774 TCATGA T^CATGA
1694 TCATGA
SapI 340 GCTCTTC GCTCTTC(1/4)
760 GCTCTTC
SmlI 2904 CTCAAG C^T[CT][GA]AG
4252 CTTGAG
StuI 1894 AGGCCT AGG^CCT
2102 AGGCCT
TaqI 379 TCGA T^CGA
735 TCGA
Tru9I 2757 TTAA T^TAA
3780 TTAA
Tth111I 464 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
476 GACTATGTC
TthHB8I 379 TCGA T^CGA
735 TCGA
Van91I 1136 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2679 CCAAGAACTGG
XhoII 2314 GGATCT [GA]^GATC[CT]
4319 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstXI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TfiI TseI Tsp45I
Tsp509I TspRI XcmI XmaCI XmaI