##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp700I AspEI AspI AviII BbrPI
BclI BfrI BglII BlpI Bpu1102I
BseAI BsiWI BsmI BspDI BspEI
BsrDI BstEII BstPI CelII ClaI
CpoI Eam1105I Eco47III EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HindIII MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I RsrII
SalI ScaI SfiI SnaBI SwaI
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AvrII 3893 CCTAGG C^CTAGG
BbeI 613 GGCGCC GGCGC^C
BcgI 3771 CGAAGACTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 3893 CCTAGG C^CTAGG
BsaAI 2722 TACGTG [CT]AC^GT[GA]
BsaBI 2026 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3152 ACCGGA [AT]^CCGG[AT]
BstBI 3769 TTCGAA TT^CGAA
DraIII 4601 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
HpaI 884 GTTAAC GTT^AAC
KasI 613 GGCGCC G^GCGCC
MamI 2026 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2066 GCCGGC GCC^GGC
NarI 613 GGCGCC GG^CGCC
NgoMIV 2066 GCCGGC G^CCGGC
NspV 3769 TTCGAA TT^CGAA
PflMI 3324 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 95 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 3864 CGATCG CGAT^CG
SbfI 2490 CCTGCAGG CCTGCA^GG
SexAI 1871 ACCAGGT A^CC[AT]GGT
SfoI 613 GGCGCC GGC^GCC
SfuI 3769 TTCGAA TT^CGAA
SgrAI 1624 CACCGGCG C[GA]^CCGG[CT]G
SmlI 4453 CTCAAG C^T[CT][GA]AG
SpeI 4168 ACTAGT A^CTAGT
SphI 3132 GCATGC GCATG^C
Sse8387I 2490 CCTGCAGG CCTGCA^GG
SspI 4231 AATATT AAT^ATT
Van91I 3324 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 198 GGTACC G^GTACC
525 GGTACC
AccI 2466 GTATAC GT^[AC][GT]AC
5162 GTATAC
Alw44I 2932 GTGCAC G^TGCAC
3286 GTGCAC
AocI 214 CCTTAGG CC^T[ACGT]AGG
3998 CCTCAGG
ApaI 294 GGGCCC GGGCC^C
437 GGGCCC
ApaLI 2932 GTGCAC G^TGCAC
3286 GTGCAC
Asp718I 198 GGTACC G^GTACC
525 GGTACC
BbvCI 3726 CCTCAGC CCTCAGC(-5/-2)
2887 GCTGAGG
BglI 2759 GCCTGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4074 GCCAGCTGGGC
BsaI 3538 GAGACC GGTCTC(1/5)
4596 GGTCTC
Bsp1407I 3817 TGTACA T^GTACA
4633 TGTACA
BspHI 3410 TCATGA T^CATGA
5168 TCATGA
BspLU11I 1664 ACATGT A^CATGT
4947 ACATGT
BsrGI 3817 TGTACA T^GTACA
4633 TGTACA
Bst1107I 2466 GTATAC GTA^TAC
5162 GTATAC
BstAPI 3108 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4819 GCACCCCCTGC
BstZ17I 2466 GTATAC GTA^TAC
5162 GTATAC
Bsu36I 214 CCTTAGG CC^T[ACGT]AGG
3998 CCTCAGG
DraI 1052 TTTAAA TTT^AAA
4141 TTTAAA
DrdI 1670 GACGAGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2488 GACCTGCAGGTC
Eco81I 214 CCTTAGG CC^T[ACGT]AGG
3998 CCTCAGG
EcoRI 2188 GAATTC G^AATTC
2642 GAATTC
KpnI 198 GGTACC GGTAC^C
525 GGTACC
RcaI 3410 TCATGA T^CATGA
5168 TCATGA
SapI 3926 GCTCTTC GCTCTTC(1/4)
4324 GCTCTTC
SspBI 3817 TGTACA T^GTACA
4633 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvI BciVI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssHII BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI