##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI Alw44I ApaLI AscI AseI
AsnI BbeI BcgI BciVI BfrI
BsaBI BsaHI BsaWI BseAI BsiWI
BsmI BspDI BspEI BspLU11I BsrBI
BssHII ClaI CpoI EagI EclXI
Eco52I EcoRI EcoRV EcoT22I FseI
Hin1I HindIII KasI MamI MfeI
MroI MunI NarI NdeI NheI
NotI NruI NsiI PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SalI SbfI SfiI SfoI SgrAI
SmaI SnaBI Sse8387I SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 1560 GGTACC G^GTACC
AhdI 2601 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 69 AGCGCT AGC^GCT
Asp718I 1560 GGTACC G^GTACC
AspEI 2601 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 419 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 344 TGCGCA TGC^GCA
BalI 1548 TGGCCA TGG^CCA
BamHI 2412 GGATCC G^GATCC
BbrPI 3087 CACGTG CAC^GTG
BbsI 4542 GAAGAC GAAGAC(2/6)
BclI 4611 TGATCA T^GATCA
BglII 4125 AGATCT A^GATCT
BlpI 1284 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1284 GCTGAGC GC^T[ACGT]AGC
BpuAI 4542 GAAGAC GAAGAC(2/6)
BsaAI 3087 CACGTG [CT]AC^GT[GA]
BsiEI 50 CGGTCG CG[GA][CT]^CG
BsmBI 219 GAGACG CGTCTC(1/5)
Bsp1407I 634 TGTACA T^GTACA
BsrGI 634 TGTACA T^GTACA
BssSI 2819 CTCGTG CACGAG(-5/-1)
Bst1107I 2284 GTATAC GTA^TAC
BstAPI 2034 GCACCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 261 TTCGAA TT^CGAA
BstZ17I 2284 GTATAC GTA^TAC
CelII 1284 GCTGAGC GC^T[ACGT]AGC
DraIII 2035 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 416 GACGACTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2601 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 69 AGCGCT AGC^GCT
FbaI 4611 TGATCA T^GATCA
FspI 344 TGCGCA TGC^GCA
HpaI 2064 GTTAAC GTT^AAC
KpnI 1560 GGTACC GGTAC^C
MluI 1693 ACGCGT A^CGCGT
MluNI 1548 TGGCCA TGG^CCA
MscI 1548 TGGCCA TGG^CCA
NaeI 101 GCCGGC GCC^GGC
NgoMIV 101 GCCGGC G^CCGGC
NspV 261 TTCGAA TT^CGAA
PaeR7I 65 CTCGAG C^TCGAG
PflFI 419 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3087 CACGTG CAC^GTG
PmlI 3087 CACGTG CAC^GTG
PshAI 2393 GACTCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1746 GAGCTC GAGCT^C
SapI 1748 GCTCTTC GCTCTTC(1/4)
ScaI 1533 AGTACT AGT^ACT
SfuI 261 TTCGAA TT^CGAA
SpeI 1227 ACTAGT A^CTAGT
SphI 3490 GCATGC GCATG^C
SspBI 634 TGTACA T^GTACA
Tth111I 419 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4230 TCTAGA T^CTAGA
XhoI 65 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 2284 GTATAC GT^[AC][GT]AC
4436 GTAGAC
Asp700I 707 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
731 GAAGGACTTC
AvrII 2717 CCTAGG C^CTAGG
3687 CCTAGG
BbvCI 2524 CCTCAGC CCTCAGC(-5/-2)
2635 CCTCAGC
BlnI 2717 CCTAGG C^CTAGG
3687 CCTAGG
BspMI 3743 ACCTGC ACCTGC(4/8)
4071 ACCTGC
BsrDI 2256 GCAATG GCAATG(2/0)
4353 GCAATG
BstEII 185 GGTTACC G^GT[ACGT]ACC
1988 GGTGACC
BstPI 185 GGTTACC G^GT[ACGT]ACC
1988 GGTGACC
EcoNI 2154 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2663 CCTGGCCCAGG
EcoO65I 185 GGTTACC G^GT[ACGT]ACC
1988 GGTGACC
HgaI 19 GACGC GACGC(5/10)
1692 GACGC
HincII 2064 GTTAAC GT[CT]^[GA]AC
4746 GTTGAC
HindII 2064 GTTAAC GT[CT]^[GA]AC
4746 GTTGAC
SexAI 2036 ACCAGGT A^CC[AT]GGT
3059 ACCAGGT
SspI 3008 AATATT AAT^ATT
3858 AATATT
XmnI 707 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
731 GAAGGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BglI
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII