##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AclI AhdI AocI Aor51HI
AscI Asp700I AspEI AspI AviII
BamHI BciVI BclI BsaBI Bst1107I
BstBI BstZ17I Bsu36I CpoI Eam1105I
Eco47III Eco81I EcoRV FbaI FseI
FspI MamI MluI NheI NotI
NruI NspV PacI PaeR7I PflFI
PmeI PshAI Psp1406I PvuI RsrII
SbfI SexAI SfiI SfuI SgrAI
SnaBI SphI Sse8387I SwaI Tth111I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 3332 GGTACC G^GTACC
AccIII 39 TCCGGA T^CCGGA
AgeI 4854 ACCGGT A^CCGGT
Alw44I 764 GTGCAC G^TGCAC
ApaI 2276 GGGCCC GGGCC^C
ApaLI 764 GTGCAC G^TGCAC
Asp718I 3332 GGTACC G^GTACC
BbvCI 45 GCTGAGG CCTCAGC(-5/-2)
BcgI 27 CGACGACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2363 AGATCT A^GATCT
BlpI 216 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 216 GCTGAGC GC^T[ACGT]AGC
BseAI 39 TCCGGA T^CCGGA
BsiWI 5057 CGTACG C^GTACG
BspEI 39 TCCGGA T^CCGGA
BssSI 2373 CTCGTG CACGAG(-5/-1)
BstEII 3190 GGTTACC G^GT[ACGT]ACC
BstPI 3190 GGTTACC G^GT[ACGT]ACC
CelII 216 GCTGAGC GC^T[ACGT]AGC
DraIII 337 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 376 GACGGCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3190 GGTTACC G^GT[ACGT]ACC
EcoRI 1741 GAATTC G^AATTC
HindIII 2706 AAGCTT A^AGCTT
HpaI 3906 GTTAAC GTT^AAC
KpnI 3332 GGTACC GGTAC^C
MfeI 2565 CAATTG C^AATTG
MroI 39 TCCGGA T^CCGGA
MunI 2565 CAATTG C^AATTG
NdeI 4453 CATATG CA^TATG
PinAI 4854 ACCGGT A^CCGGT
SalI 1521 GTCGAC G^TCGAC
ScaI 2333 AGTACT AGT^ACT
SpeI 3458 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 1521 GTCGAC GT^[AC][GT]AC
3510 GTAGAC
AlwNI 1699 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1801 CAGGTCCTG
AseI 3623 ATTAAT AT^TAAT
4981 ATTAAT
AsnI 3623 ATTAAT AT^TAAT
4981 ATTAAT
AvaI 175 CCCGGG C^[CT]CG[GA]G
206 CCCGGG
AvrII 3336 CCTAGG C^CTAGG
4199 CCTAGG
BbeI 379 GGCGCC GGCGC^C
393 GGCGCC
BbrPI 2785 CACGTG CAC^GTG
4436 CACGTG
BbsI 3060 GTCTTC GAAGAC(2/6)
5449 GTCTTC
BlnI 3336 CCTAGG C^CTAGG
4199 CCTAGG
BpuAI 3060 GTCTTC GAAGAC(2/6)
5449 GTCTTC
BsaI 3341 GGTCTC GGTCTC(1/5)
4763 GGTCTC
BsoBI 175 CCCGGG C^[CT]CG[GA]G
206 CCCGGG
BspDI 568 ATCGAT AT^CGAT
2537 ATCGAT
BsrBI 1728 CCGCTC CCGCTC(-3/-3)
970 GAGCGG
BssHII 22 GCGCGC G^CGCGC
306 GCGCGC
BstXI 581 CCAACACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3022 CCAGTGCCCTGG
ClaI 568 ATCGAT AT^CGAT
2537 ATCGAT
EcoNI 1806 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2684 CCTGACTAAGG
EcoT22I 1789 ATGCAT ATGCA^T
3900 ATGCAT
KasI 379 GGCGCC G^GCGCC
393 GGCGCC
NarI 379 GGCGCC GG^CGCC
393 GGCGCC
NcoI 2192 CCATGG C^CATGG
4916 CCATGG
NsiI 1789 ATGCAT ATGCA^T
3900 ATGCAT
PmaCI 2785 CACGTG CAC^GTG
4436 CACGTG
PmlI 2785 CACGTG CAC^GTG
4436 CACGTG
PpuMI 1802 AGGTCCT [GA]G^G[AT]CC[CT]
2022 GGGACCT
PshBI 3623 ATTAAT AT^TAAT
4981 ATTAAT
PvuII 2384 CAGCTG CAG^CTG
3227 CAGCTG
SacI 787 GAGCTC GAGCT^C
3258 GAGCTC
SfoI 379 GGCGCC GGC^GCC
393 GGCGCC
SmaI 175 CCCGGG CCC^GGG
206 CCCGGG
SspI 1215 AATATT AAT^ATT
4984 AATATT
StuI 636 AGGCCT AGG^CCT
4844 AGGCCT
XcmI 89 CCACCCGCGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2165 CCAAAGAGTTGATGG
XmaCI 175 CCCGGG C^CCGGG
206 CCCGGG
XmaI 175 CCCGGG C^CCGGG
206 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AflIII AluI AlwI ApoI
AspHI AvaII BalI BanI BanII
BbvI BcnI BfaI BfrI BglI
BmyI BpmI BsaAI BsaHI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI Bsp1286I Bsp1407I BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PstI RcaI RsaI SacII
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII