##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AccI AclI AgeI Aor51HI
AscI Asp700I Asp718I AspI AviII
BclI BglII BsaBI BsiWI BspDI
BspHI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DrdI Eco47III
EcoRV FbaI FspI HincII HindII
HindIII HpaI KpnI MamI MfeI
MluI MunI NheI NotI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI Psp1406I PvuI RcaI RsrII
SalI SexAI SfuI SnaBI SpeI
SwaI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 2519 GACGTC GACGT^C
AccIII 264 TCCGGA T^CCGGA
AhdI 2889 GACCCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 5594 ATTAAT AT^TAAT
AsnI 5594 ATTAAT AT^TAAT
AspEI 2889 GACCCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 4938 TGGCCA TGG^CCA
BbrPI 4590 CACGTG CAC^GTG
BciVI 257 GGATAC GTATCC(6/5)
BsaAI 4590 CACGTG [CT]AC^GT[GA]
BseAI 264 TCCGGA T^CCGGA
BsmI 3993 GCATTC GAATGC(1/-1)
Bsp1407I 1762 TGTACA T^GTACA
BspEI 264 TCCGGA T^CCGGA
BspLU11I 1759 ACATGT A^CATGT
BsrGI 1762 TGTACA T^GTACA
BstEII 489 GGTCACC G^GT[ACGT]ACC
BstPI 489 GGTCACC G^GT[ACGT]ACC
Eam1105I 2889 GACCCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 489 GGTCACC G^GT[ACGT]ACC
EcoRI 5838 GAATTC G^AATTC
EcoT22I 1920 ATGCAT ATGCA^T
FseI 2286 GGCCGGCC GGCCGG^CC
MluNI 4938 TGGCCA TGG^CCA
MroI 264 TCCGGA T^CCGGA
MscI 4938 TGGCCA TGG^CCA
NdeI 520 CATATG CA^TATG
NruI 804 TCGCGA TCG^CGA
NsiI 1920 ATGCAT ATGCA^T
PmaCI 4590 CACGTG CAC^GTG
PmlI 4590 CACGTG CAC^GTG
PshBI 5594 ATTAAT AT^TAAT
SbfI 4984 CCTGCAGG CCTGCA^GG
SfiI 2031 GGCCCTGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2303 CGCCGGTG C[GA]^CCGG[CT]G
Sse8387I 4984 CCTGCAGG CCTGCA^GG
SspBI 1762 TGTACA T^GTACA
StuI 5242 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AflII 4146 CTTAAG C^TTAAG
5081 CTTAAG
AflIII 1759 ACATGT A^C[GA][CT]GT
4589 ACACGT
Alw44I 3777 GTGCAC G^TGCAC
4449 GTGCAC
ApaLI 3777 GTGCAC G^TGCAC
4449 GTGCAC
BamHI 2353 GGATCC G^GATCC
4880 GGATCC
BcgI 1302 CGAGAACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1225 GCAAGTACTTCG
BfrI 4146 CTTAAG C^TTAAG
5081 CTTAAG
BsrDI 5351 CATTGC GCAATG(2/0)
3565 GCAATG
HgaI 141 GACGC GACGC(5/10)
3015 GACGC
NcoI 2026 CCATGG C^CATGG
4398 CCATGG
SacI 1464 GAGCTC GAGCT^C
3575 GAGCTC
SphI 554 GCATGC GCATG^C
4561 GCATGC
SspI 5751 AATATT AAT^ATT
5807 AATATT
XbaI 4435 TCTAGA T^CTAGA
4615 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII AvrII
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BglI BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacII SapI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SfoI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI