##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccI AflII AgeI AscI
AseI AsnI AviII AvrII BbvCI
BciVI BclI BfrI BglII BlnI
BsiWI BsmBI BspDI BspLU11I BsrDI
Bst1107I BstEII BstPI BstZ17I ClaI
CpoI DraIII DrdI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KspI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI PacI PinAI PmeI PshBI
PvuI RsrII SacII SalI SbfI
ScaI SexAI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 465 GGTACC G^GTACC
AclI 4751 AACGTT AA^CGTT
AflIII 4339 ACGTGT A^C[GA][CT]GT
Aor51HI 1573 AGCGCT AGC^GCT
Asp718I 465 GGTACC G^GTACC
AspI 451 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 4783 CACGTG CAC^GTG
BbsI 2590 GAAGAC GAAGAC(2/6)
BcgI 717 CGACGGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 2590 GAAGAC GAAGAC(2/6)
BsaAI 4783 CACGTG [CT]AC^GT[GA]
BsaBI 693 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 4308 TGTACA T^GTACA
BsrGI 4308 TGTACA T^GTACA
BstAPI 1380 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4756 TTCGAA TT^CGAA
BstXI 1841 CCAAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 4382 TTTAAA TTT^AAA
Eco47III 1573 AGCGCT AGC^GCT
KpnI 465 GGTACC GGTAC^C
MamI 693 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspV 4756 TTCGAA TT^CGAA
PaeR7I 4183 CTCGAG C^TCGAG
PflFI 451 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4783 CACGTG CAC^GTG
PmlI 4783 CACGTG CAC^GTG
PshAI 1852 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4751 AACGTT AA^CGTT
SfiI 1002 GGCCGGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 4756 TTCGAA TT^CGAA
SspBI 4308 TGTACA T^GTACA
StuI 599 AGGCCT AGG^CCT
Tth111I 451 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4183 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 2162 TCCGGA T^CCGGA
2762 TCCGGA
AcsI 346 GAATTT [GA]^AATT[CT]
1320 AAATTC
AhdI 3727 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4067 GACGAAGGGTC
Alw44I 814 GTGCAC G^TGCAC
3213 GTGCAC
AlwNI 1243 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1381 CAGGGCCTG
ApaLI 814 GTGCAC G^TGCAC
3213 GTGCAC
ApoI 346 GAATTT [GA]^AATT[CT]
1320 AAATTC
AspEI 3727 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4067 GACGAAGGGTC
BamHI 1156 GGATCC G^GATCC
3351 GGATCC
BbeI 2564 GGCGCC GGCGC^C
3523 GGCGCC
BseAI 2162 TCCGGA T^CCGGA
2762 TCCGGA
BsmI 250 GAATGC GAATGC(1/-1)
4215 GCATTC
BspEI 2162 TCCGGA T^CCGGA
2762 TCCGGA
BspHI 557 TCATGA T^CATGA
1598 TCATGA
BspMI 1709 ACCTGC ACCTGC(4/8)
2456 ACCTGC
BssSI 3446 CACGAG CACGAG(-5/-1)
1962 CTCGTG
BstYI 1156 GGATCC [GA]^GATC[CT]
3351 GGATCC
EagI 64 CGGCCG C^GGCCG
98 CGGCCG
Eam1105I 3727 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4067 GACGAAGGGTC
EclXI 64 CGGCCG C^GGCCG
98 CGGCCG
Eco52I 64 CGGCCG C^GGCCG
98 CGGCCG
HincII 329 GTCAAC GT[CT]^[GA]AC
4075 GTCAAC
HindII 329 GTCAAC GT[CT]^[GA]AC
4075 GTCAAC
KasI 2564 GGCGCC G^GCGCC
3523 GGCGCC
MflI 1156 GGATCC [GA]^GATC[CT]
3351 GGATCC
MroI 2162 TCCGGA T^CCGGA
2762 TCCGGA
NarI 2564 GGCGCC GG^CGCC
3523 GGCGCC
NcoI 869 CCATGG C^CATGG
1847 CCATGG
NspI 2528 GCATGT [GA]CATG^[CT]
4802 GCATGT
PflMI 3199 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3659 CCAGCCCGTGG
RcaI 557 TCATGA T^CATGA
1598 TCATGA
SacI 2583 GAGCTC GAGCT^C
4026 GAGCTC
SapI 2799 GAAGAGC GCTCTTC(1/4)
3804 GCTCTTC
SfoI 2564 GGCGCC GGC^GCC
3523 GGCGCC
SmlI 2668 CTCAAG C^T[CT][GA]AG
4183 CTCGAG
Van91I 3199 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3659 CCAGCCCGTGG
XhoII 1156 GGATCC [GA]^GATC[CT]
3351 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AocI ApaI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XmaCI
XmaI XmnI