##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
Acc65I AccIII AclI AgeI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AviII BbeI BbrPI BcgI BsaAI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoT22I FseI FspI
KasI KpnI KspI MluI MroI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfoI SfuI SnaBI
SpeI SphI Sse8387I SspI SwaI
XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 2353 GACGTC GACGT^C
AcyI 2353 GACGTC G[GA]^CG[CT]C
AflII 2695 CTTAAG C^TTAAG
AocI 320 CCTGAGG CC^T[ACGT]AGG
AseI 1481 ATTAAT AT^TAAT
AsnI 1481 ATTAAT AT^TAAT
AvaI 95 CCCGGG C^[CT]CG[GA]G
BamHI 721 GGATCC G^GATCC
BbvCI 33 GCTGAGG CCTCAGC(-5/-2)
BciVI 4578 GGATAC GTATCC(6/5)
BfrI 2695 CTTAAG C^TTAAG
BglI 2184 GCCTAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 2353 GACGTC G[GA]^CG[CT]C
BseRI 4671 CTCCTC GAGGAG(10/8)
BsoBI 95 CCCGGG C^[CT]CG[GA]G
BsrFI 149 GCCGGT [GA]^CCGG[CT]
BstEII 862 GGTGACC G^GT[ACGT]ACC
BstPI 862 GGTGACC G^GT[ACGT]ACC
Bsu36I 320 CCTGAGG CC^T[ACGT]AGG
Cfr10I 149 GCCGGT [GA]^CCGG[CT]
Eco81I 320 CCTGAGG CC^T[ACGT]AGG
EcoO65I 862 GGTGACC G^GT[ACGT]ACC
EcoRI 2997 GAATTC G^AATTC
EcoRV 4215 GATATC GAT^ATC
FauI 175 GCGGG CCCGC(4/6)
HaeII 188 AGCGCC [GA]GCGC^[CT]
HgaI 382 GCGTC GACGC(5/10)
Hin1I 2353 GACGTC G[GA]^CG[CT]C
HindIII 844 AAGCTT A^AGCTT
MfeI 1474 CAATTG C^AATTG
MunI 1474 CAATTG C^AATTG
NcoI 1944 CCATGG C^CATGG
PflMI 5130 CCAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1481 ATTAAT AT^TAAT
SapI 1166 GAAGAGC GCTCTTC(1/4)
ScaI 2972 AGTACT AGT^ACT
SexAI 5106 ACCTGGT A^CC[AT]GGT
SgrAI 148 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 95 CCCGGG CCC^GGG
StuI 2715 AGGCCT AGG^CCT
Van91I 5130 CCAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 95 CCCGGG C^CCGGG
XmaI 95 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 146 CGCG CG^CG
381 CGCG
AhdI 992 GACTTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1439 GACATTAAGTC
Asp700I 1054 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1271 GAAGAAGTTC
AspEI 992 GACTTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1439 GACATTAAGTC
AspI 865 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
4550 GACTTTGTC
BclI 2911 TGATCA T^GATCA
4110 TGATCA
BglII 2489 AGATCT A^GATCT
3317 AGATCT
BsaBI 3523 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4579 GATACCCATC
BsgI 1011 GTGCAG GTGCAG(16/14)
4319 GTGCAG
BspHI 805 TCATGA T^CATGA
2357 TCATGA
BsrDI 3752 CATTGC GCAATG(2/0)
2371 GCAATG
BstUI 146 CGCG CG^CG
381 CGCG
BstXI 2588 CCAAGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4812 CCAGAAACATGG
DsaI 1728 CCACGG C^C[GA][CT]GG
1944 CCATGG
Eam1105I 992 GACTTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1439 GACATTAAGTC
FbaI 2911 TGATCA T^GATCA
4110 TGATCA
MaeII 1454 ACGT A^CGT
2354 ACGT
MamI 3523 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4579 GATACCCATC
MvnI 146 CGCG CG^CG
381 CGCG
NdeI 2609 CATATG CA^TATG
5241 CATATG
PflFI 865 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
4550 GACTTTGTC
PpuMI 598 AGGTCCT [GA]G^G[AT]CC[CT]
1113 AGGACCT
RcaI 805 TCATGA T^CATGA
2357 TCATGA
Tth111I 865 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
4550 GACTTTGTC
XmnI 1054 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1271 GAAGAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII AvrII BalI BanI BanII
BbsI BbvI BcnI BfaI BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspLU11I BspMI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII