##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AccIII AclI AcsI AflII AhdI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I AspEI AvrII BamHI
BbeI BfaI BfrI BlnI BsaBI
BseAI BsiWI BsmI BspDI BspEI
BspHI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
HindIII HpaI KasI MaeI MamI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PmeI PshBI
Psp1406I PvuI RcaI RsrII SalI
SfoI SfuI SgrAI SnaBI SpeI
SspI SwaI TfiI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 10 enzymes) #
###################################
BbvCI 1917 CCTCAGC CCTCAGC(-5/-2)
BcgI 3374 GCACGGATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4205 GTATCC GTATCC(6/5)
BclI 4850 TGATCA T^GATCA
BglII 2387 AGATCT A^GATCT
BspLU11I 491 ACATGT A^CATGT
BsrDI 1578 CATTGC GCAATG(2/0)
DraI 5989 TTTAAA TTT^AAA
FbaI 4850 TGATCA T^GATCA
SfiI 435 GGCCAAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 2179 GACGTC GACGT^C
2880 GACGTC
Acc65I 1652 GGTACC G^GTACC
4613 GGTACC
AgeI 3083 ACCGGT A^CCGGT
4829 ACCGGT
Asp718I 1652 GGTACC G^GTACC
4613 GGTACC
AviII 2937 TGCGCA TGC^GCA
3176 TGCGCA
BbrPI 349 CACGTG CAC^GTG
735 CACGTG
BbsI 222 GAAGAC GAAGAC(2/6)
3439 GTCTTC
BglI 436 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3760 GCCACCGTGGC
BpuAI 222 GAAGAC GAAGAC(2/6)
3439 GTCTTC
BsaWI 3083 ACCGGT [AT]^CCGG[AT]
4829 ACCGGT
BsiEI 3066 CGACCG CG[GA][CT]^CG
4788 CGGTCG
Bsp1407I 4457 TGTACA T^GTACA
5965 TGTACA
BsrGI 4457 TGTACA T^GTACA
5965 TGTACA
BssHII 9 GCGCGC G^CGCGC
1477 GCGCGC
BssSI 2590 CTCGTG CACGAG(-5/-1)
3445 CTCGTG
DrdI 712 GACGCCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5041 GACTACTCGGTC
FspI 2937 TGCGCA TGC^GCA
3176 TGCGCA
KpnI 1652 GGTACC GGTAC^C
4613 GGTACC
KspI 3975 CCGCGG CCGC^GG
5805 CCGCGG
MseI 5562 TTAA T^TAA
5990 TTAA
PinAI 3083 ACCGGT A^CCGGT
4829 ACCGGT
PmaCI 349 CACGTG CAC^GTG
735 CACGTG
PmlI 349 CACGTG CAC^GTG
735 CACGTG
PshAI 4173 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5389 GACAGAAGTC
SacII 3975 CCGCGG CCGC^GG
5805 CCGCGG
SbfI 2829 CCTGCAGG CCTGCA^GG
5785 CCTGCAGG
ScaI 596 AGTACT AGT^ACT
3911 AGTACT
SexAI 1058 ACCAGGT A^CC[AT]GGT
3548 ACCTGGT
SphI 3221 GCATGC GCATG^C
4064 GCATGC
Sse8387I 2829 CCTGCAGG CCTGCA^GG
5785 CCTGCAGG
SspBI 4457 TGTACA T^GTACA
5965 TGTACA
Tru9I 5562 TTAA T^TAA
5990 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaI ApaLI AspHI AspI AvaI
AvaII BalI BanI BanII BbvI
BcnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmaCI XmaI