##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       AscI        Asp718I     AspEI       AvaII       
AviII       AvrII       BalI        BamHI       BanII       
BglI        BlnI        BlpI        Bpu1102I    BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspMI       BsrBI       
BsrFI       BsrGI       BssHII      BstAPI      BstUI       
CelII       Cfr10I      Cfr13I      ClaI        CpoI        
DraII       DraIII      DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO109I    
EcoRV       FseI        FspI        KpnI        KspI        
MluI        MluNI       MroI        MscI        MvnI        
NaeI        NcoI        NgoMIV      NheI        NotI        
NruI        PacI        PaeR7I      PflMI       PinAI       
PmeI        PpuMI       PshAI       PstI        PvuI        
RsrII       SacI        SacII       Sau96I      SbfI        
SexAI       SfiI        SgrAI       SphI        Sse8387I    
SspBI       StuI        Van91I      XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AclI         4511  AACGTT               AA^CGTT
AcyI         2337  GGCGCC               G[GA]^CG[CT]C
AlwI         1027  GGATC                GGATC(4/5)
AspI         3297  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         4392  CCCGGG               C^[CT]CG[GA]G
BbeI         2337  GGCGCC               GGCGC^C
BbrPI        1386  CACGTG               CAC^GTG
BcgI         1358  CGAAGATCTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         4819  TGATCA               T^GATCA
BsaHI        2337  GGCGCC               G[GA]^CG[CT]C
BsoBI        4392  CCCGGG               C^[CT]CG[GA]G
BssSI        3104  CACGAG               CACGAG(-5/-1)
BstBI        2938  TTCGAA               TT^CGAA
BstEII       1263  GGTCACC              G^GT[ACGT]ACC
BstPI        1263  GGTCACC              G^GT[ACGT]ACC
DrdI         2624  GACTGGGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1597  CCTAAGAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1263  GGTCACC              G^GT[ACGT]ACC
EcoT22I        48  ATGCAT               ATGCA^T
FauI         4337  GCGGG                CCCGC(4/6)
FbaI         4819  TGATCA               T^GATCA
HaeII        2337  GGCGCC               [GA]GCGC^[CT]
HgaI         3292  GCGTC                GACGC(5/10)
Hin1I        2337  GGCGCC               G[GA]^CG[CT]C
HindIII      1707  AAGCTT               A^AGCTT
KasI         2337  GGCGCC               G^GCGCC
MfeI         2930  CAATTG               C^AATTG
MunI         2930  CAATTG               C^AATTG
NarI         2337  GGCGCC               GG^CGCC
NsiI           48  ATGCAT               ATGCA^T
NspV         2938  TTCGAA               TT^CGAA
PflFI        3297  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1386  CACGTG               CAC^GTG
PmlI         1386  CACGTG               CAC^GTG
Psp1406I     4511  AACGTT               AA^CGTT
SalI         3294  GTCGAC               G^TCGAC
SfoI         2337  GGCGCC               GGC^GCC
SfuI         2938  TTCGAA               TT^CGAA
SmaI         4392  CCCGGG               CCC^GGG
SnaBI          27  TACGTA               TAC^GTA
SpeI         3167  ACTAGT               A^CTAGT
Tth111I      3297  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        4392  CCCGGG               C^CCGGG
XmaI         4392  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AocI           58  CCTAAGG              CC^T[ACGT]AGG
             4251  CCTAAGG             
Asp700I       534  GAAAAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3892  GAATATATTC          
AspHI        2750  GAGCAC               G[AT]GC[AT]^C
             4923  GTGCTC              
BanI         2337  GGCGCC               G^G[CT][GA]CC
             4340  GGCACC              
BbvCI        2648  GCTGAGG              CCTCAGC(-5/-2)
             4368  GCTGAGG             
BciVI        3112  GGATAC               GTATCC(6/5)
             5030  GTATCC              
BsaAI          27  TACGTA               [CT]AC^GT[GA]
             1386  CACGTG              
BsaBI        1991  GATGACTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2254  GATAATGATC          
BsaI         4268  GAGACC               GGTCTC(1/5)
             3336  GGTCTC              
BsgI          608  CTGCAC               GTGCAG(16/14)
             4194  GTGCAG              
BsiHKAI      2750  GAGCAC               G[AT]GC[AT]^C
             4923  GTGCTC              
BspHI        3469  TCATGA               T^CATGA
             3633  TCATGA              
BspLU11I      450  ACATGT               A^CATGT
             1790  ACATGT              
BsrDI        4429  CATTGC               GCAATG(2/0)
             4281  GCAATG              
Bsu36I         58  CCTAAGG              CC^T[ACGT]AGG
             4251  CCTAAGG             
CfoI         1383  GCGC                 GCG^C
             2338  GCGC                
EarI         2503  GAAGAG               CTCTTC(1/4)
             2822  GAAGAG              
Eco81I         58  CCTAAGG              CC^T[ACGT]AGG
             4251  CCTAAGG             
EcoRI        2935  GAATTC               G^AATTC
             5050  GAATTC              
EcoT14I       840  CCAAGG               C^C[AT][AT]GG
             2562  CCAAGG              
HaeIII       4188  GGCC                 GG^CC
             4232  GGCC                
HhaI         1383  GCGC                 GCG^C
             2338  GCGC                
HinP1I       1383  GCGC                 G^CGC
             2338  GCGC                
HpaI         3142  GTTAAC               GTT^AAC
             4018  GTTAAC              
Ksp632I      2503  GAAGAG               CTCTTC(1/4)
             2822  GAAGAG              
MamI         1991  GATGACTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2254  GATAATGATC          
NdeI         4030  CATATG               CA^TATG
             5101  CATATG              
NlaIV        2337  GGCGCC               GG[ACGT]^[ACGT]CC
             4340  GGCACC              
NspI          450  ACATGT               [GA]CATG^[CT]
             1790  ACATGT              
PvuII        1055  CAGCTG               CAG^CTG
             2288  CAGCTG              
RcaI         3469  TCATGA               T^CATGA
             3633  TCATGA              
SapI         2503  GAAGAGC              GCTCTTC(1/4)
             2822  GAAGAGC             
ScaI         3451  AGTACT               AGT^ACT
             3750  AGTACT              
StyI          840  CCAAGG               C^C[AT][AT]GG
             2562  CCAAGG              
SwaI          228  ATTTAAAT             ATTT^AAAT
             3508  ATTTAAAT            
XmnI          534  GAAAAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3892  GAATATATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflII       
AflIII      AluI        ApoI        AseI        AsnI        
BbsI        BbvI        BcnI        BfaI        BfrI        
BglII       BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       Bst1107I    BstF5I      
BstNI       BstXI       BstYI       BstZ17I     Cac8I       
DdeI        DpnII       DraI        EcoRII      Fnu4HI      
FokI        HapII       HincII      HindII      HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      PleI        
PshBI       RsaI        Sau3AI      ScrFI       SfaNI       
SfcI        SmlI        SspI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XbaI        XhoII