##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AspEI AspI
AviII AvrII BbeI BbvCI BcgI
BlnI BsaHI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BsrGI
BssHII BssSI BstEII BstPI BstUI
Bsu36I ClaI CpoI DraIII DrdI
DsaI Eam1105I Eco47III Eco81I EcoO65I
EcoRV FseI FspI Hin1I KasI
KspI MluI MroI MvnI NaeI
NarI NcoI NgoMIV NotI NruI
PacI PflFI PflMI PinAI PmeI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfoI SgrAI SmaI
Sse8387I SspBI Tth111I Van91I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
BalI 4098 TGGCCA TGG^CCA
BamHI 2051 GGATCC G^GATCC
BbrPI 3807 CACGTG CAC^GTG
BciVI 4714 GTATCC GTATCC(6/5)
BcnI 4625 CCCGG CC^[GC]GG
BsaBI 4969 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 5549 GAGACC GGTCTC(1/5)
BsrBI 3359 GAGCGG CCGCTC(-3/-3)
BsrFI 3919 ACCGGC [GA]^CCGG[CT]
CfoI 3651 GCGC GCG^C
Cfr10I 3919 ACCGGC [GA]^CCGG[CT]
EagI 4703 CGGCCG C^GGCCG
EclXI 4703 CGGCCG C^GGCCG
Eco52I 4703 CGGCCG C^GGCCG
HaeII 3650 AGCGCC [GA]GCGC^[CT]
HgaI 3099 GACGC GACGC(5/10)
HhaI 3651 GCGC GCG^C
HinP1I 3651 GCGC G^CGC
HpaI 755 GTTAAC GTT^AAC
MamI 4969 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 563 CAATTG C^AATTG
MluNI 4098 TGGCCA TGG^CCA
MscI 4098 TGGCCA TGG^CCA
MunI 563 CAATTG C^AATTG
NciI 4625 CCCGG CC^[GC]GG
NdeI 92 CATATG CA^TATG
NheI 2068 GCTAGC G^CTAGC
PmaCI 3807 CACGTG CAC^GTG
PmlI 3807 CACGTG CAC^GTG
PshAI 2126 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 4690 ACCAGGT A^CC[AT]GGT
SnaBI 4890 TACGTA TAC^GTA
SpeI 1262 ACTAGT A^CTAGT
StuI 1692 AGGCCT AGG^CCT
SwaI 1167 ATTTAAAT ATTT^AAAT
XbaI 4656 TCTAGA T^CTAGA
XcmI 882 CCATAATTAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 2009 GGTACC G^GTACC
4949 GGTACC
AflII 1600 CTTAAG C^TTAAG
4227 CTTAAG
Asp718I 2009 GGTACC G^GTACC
4949 GGTACC
AvaI 1475 CTCGAG C^[CT]CG[GA]G
5453 CTCGAG
BbsI 508 GAAGAC GAAGAC(2/6)
5526 GAAGAC
BfrI 1600 CTTAAG C^TTAAG
4227 CTTAAG
BglI 4606 GCCACCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4676 GCCCAAAGGGC
BglII 3642 AGATCT A^GATCT
4908 AGATCT
BlpI 2821 GCTCAGC GC^T[ACGT]AGC
4180 GCTGAGC
Bpu1102I 2821 GCTCAGC GC^T[ACGT]AGC
4180 GCTGAGC
BpuAI 508 GAAGAC GAAGAC(2/6)
5526 GAAGAC
BsaAI 3807 CACGTG [CT]AC^GT[GA]
4890 TACGTA
BseRI 2187 GAGGAG GAGGAG(10/8)
3911 GAGGAG
BsgI 5464 CTGCAC GTGCAG(16/14)
4820 GTGCAG
BsiEI 4699 CGGTCG CG[GA][CT]^CG
4703 CGGCCG
BsoBI 1475 CTCGAG C^[CT]CG[GA]G
5453 CTCGAG
Bst1107I 4420 GTATAC GTA^TAC
5249 GTATAC
BstAPI 1511 GCAGAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2690 GCACTGCATGC
BstBI 3324 TTCGAA TT^CGAA
4535 TTCGAA
BstXI 1744 CCAGACAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3802 CCAACCACGTGG
BstZ17I 4420 GTATAC GTA^TAC
5249 GTATAC
CelII 2821 GCTCAGC GC^T[ACGT]AGC
4180 GCTGAGC
DraII 3429 AGGACCT [GA]G^G[ACGT]CC[CT]
4924 AGGTCCC
EcoO109I 3429 AGGACCT [GA]G^G[ACGT]CC[CT]
4924 AGGTCCC
EcoT14I 594 CCTTGG C^C[AT][AT]GG
4610 CCTTGG
EcoT22I 1256 ATGCAT ATGCA^T
3616 ATGCAT
FauI 129 CCCGC CCCGC(4/6)
3361 GCGGG
HapII 3920 CCGG C^CGG
4626 CCGG
HincII 755 GTTAAC GT[CT]^[GA]AC
4202 GTCAAC
HindII 755 GTTAAC GT[CT]^[GA]AC
4202 GTCAAC
HindIII 2358 AAGCTT A^AGCTT
3955 AAGCTT
HpaII 3920 CCGG C^CGG
4626 CCGG
KpnI 2009 GGTACC GGTAC^C
4949 GGTACC
MspI 3920 CCGG C^CGG
4626 CCGG
NsiI 1256 ATGCAT ATGCA^T
3616 ATGCAT
NspV 3324 TTCGAA TT^CGAA
4535 TTCGAA
PaeR7I 1475 CTCGAG C^TCGAG
5453 CTCGAG
PpuMI 3429 AGGACCT [GA]G^G[AT]CC[CT]
4924 AGGTCCC
ScaI 4135 AGTACT AGT^ACT
5604 AGTACT
SfuI 3324 TTCGAA TT^CGAA
4535 TTCGAA
SphI 2265 GCATGC GCATG^C
2695 GCATGC
StyI 594 CCTTGG C^C[AT][AT]GG
4610 CCTTGG
Tsp45I 4489 GTCAC ^GT[GC]AC
4765 GTCAC
XhoI 1475 CTCGAG C^TCGAG
5453 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaII BanI
BanII BbvI BclI BfaI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BspLU11I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI EaeI EarI
EcoNI EcoRI EcoRII FbaI Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PshBI PstI
PvuII RcaI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII
XmnI