##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AclI AcyI AflII AhdI
Aor51HI AseI AsnI AspEI AviII
AvrII BbeI BbrPI BfrI BlnI
BsaHI BsiEI BsiWI Bsp1407I BspDI
BspHI BsrDI BsrGI Bst1107I BstBI
BstZ17I ClaI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV FseI FspI
HgaI Hin1I KasI MfeI MluI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SfoI
SfuI SgrAI SnaBI SspBI SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 2219 GGTACC G^GTACC
AflIII 2933 ACATGT A^C[GA][CT]GT
AgeI 3651 ACCGGT A^CCGGT
Alw44I 4720 GTGCAC G^TGCAC
ApaLI 4720 GTGCAC G^TGCAC
AscI 13 GGCGCGCC GG^CGCGCC
Asp700I 1523 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2219 GGTACC G^GTACC
AspI 32 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 5239 GGATCC G^GATCC
BcgI 4223 CGAAAAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 816 GTATCC GTATCC(6/5)
BglII 405 AGATCT A^GATCT
BlpI 1886 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1886 GCTGAGC GC^T[ACGT]AGC
BsaAI 3453 CACGTA [CT]AC^GT[GA]
BsaBI 4518 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 30 GAGACG CGTCTC(1/5)
BspLU11I 2933 ACATGT A^CATGT
BssHII 14 GCGCGC G^CGCGC
BssSI 2135 CTCGTG CACGAG(-5/-1)
CelII 1886 GCTGAGC GC^T[ACGT]AGC
CpoI 64 CGGTCCG CG^G[AT]CCG
DraI 5157 TTTAAA TTT^AAA
DraIII 3209 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 1606 GAATTC G^AATTC
HindIII 668 AAGCTT A^AGCTT
HpaI 5200 GTTAAC GTT^AAC
KpnI 2219 GGTACC GGTAC^C
KspI 88 CCGCGG CCGC^GG
MamI 4518 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflFI 32 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3651 ACCGGT A^CCGGT
RsrII 64 CGGTCCG CG^G[AT]CCG
SacII 88 CCGCGG CCGC^GG
SalI 1736 GTCGAC G^TCGAC
ScaI 2731 AGTACT AGT^ACT
SexAI 1357 ACCAGGT A^CC[AT]GGT
SfiI 4268 GGCCGCCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 1154 ACTAGT A^CTAGT
SphI 2667 GCATGC GCATG^C
StuI 4143 AGGCCT AGG^CCT
Tth111I 32 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1669 TCTAGA T^CTAGA
XmnI 1523 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 67 TCCGGA T^CCGGA
3906 TCCGGA
AcsI 1606 GAATTC [GA]^AATT[CT]
4940 AAATTT
ApoI 1606 GAATTC [GA]^AATT[CT]
4940 AAATTT
BclI 1899 TGATCA T^GATCA
4069 TGATCA
BseAI 67 TCCGGA T^CCGGA
3906 TCCGGA
BsmI 450 GCATTC GAATGC(1/-1)
5208 GCATTC
BspEI 67 TCCGGA T^CCGGA
3906 TCCGGA
BsrBI 4510 CCGCTC CCGCTC(-3/-3)
145 GAGCGG
BstEII 1219 GGTAACC G^GT[ACGT]ACC
4998 GGTAACC
BstPI 1219 GGTAACC G^GT[ACGT]ACC
4998 GGTAACC
DrdI 1641 GACAAAGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3896 GACTATTGTGTC
EcoNI 319 CCTCATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5068 CCTACTCTAGG
EcoO65I 1219 GGTAACC G^GT[ACGT]ACC
4998 GGTAACC
EcoT22I 833 ATGCAT ATGCA^T
4042 ATGCAT
FbaI 1899 TGATCA T^GATCA
4069 TGATCA
MroI 67 TCCGGA T^CCGGA
3906 TCCGGA
MslI 4730 CACTTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
5209 CATTCCTATG
NcoI 3139 CCATGG C^CATGG
3856 CCATGG
NsiI 833 ATGCAT ATGCA^T
4042 ATGCAT
PflMI 890 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5245 CCAGTGTTTGG
SacI 1371 GAGCTC GAGCT^C
3144 GAGCTC
SapI 1393 GAAGAGC GCTCTTC(1/4)
4443 GCTCTTC
SbfI 2651 CCTGCAGG CCTGCA^GG
4230 CCTGCAGG
Sse8387I 2651 CCTGCAGG CCTGCA^GG
4230 CCTGCAGG
Van91I 890 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5245 CCAGTGTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI