##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AccIII Aor51HI AscI Asp718I
BamHI BciVI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
Bst1107I BstEII BstPI BstZ17I ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoO65I EcoT22I FseI HincII HindII
HpaI KpnI MroI NheI NotI
NruI NsiI PacI PaeR7I PmeI
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfiI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AclI 5190 AACGTT AA^CGTT
AgeI 2173 ACCGGT A^CCGGT
AhdI 57 GACGATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 263 GTGCAC G^TGCAC
ApaLI 263 GTGCAC G^TGCAC
AseI 1615 ATTAAT AT^TAAT
AsnI 1615 ATTAAT AT^TAAT
AspEI 57 GACGATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI 4619 TGATCA T^GATCA
BsaBI 2027 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1414 CGTCTC CGTCTC(1/5)
BsmI 5177 GCATTC GAATGC(1/-1)
BsrBI 3901 CCGCTC CCGCTC(-3/-3)
BstBI 2071 TTCGAA TT^CGAA
DraI 4579 TTTAAA TTT^AAA
DraIII 4954 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4900 GACGCGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 57 GACGATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2164 GAATTC G^AATTC
EcoRV 4938 GATATC GAT^ATC
FbaI 4619 TGATCA T^GATCA
HindIII 4574 AAGCTT A^AGCTT
KspI 3637 CCGCGG CCGC^GG
MamI 2027 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5228 CAATTG C^AATTG
MunI 5228 CAATTG C^AATTG
NaeI 40 GCCGGC GCC^GGC
NdeI 4386 CATATG CA^TATG
NgoMIV 40 GCCGGC G^CCGGC
NspV 2071 TTCGAA TT^CGAA
PinAI 2173 ACCGGT A^CCGGT
PshBI 1615 ATTAAT AT^TAAT
Psp1406I 5190 AACGTT AA^CGTT
PvuII 1351 CAGCTG CAG^CTG
SacII 3637 CCGCGG CCGC^GG
SfuI 2071 TTCGAA TT^CGAA
SphI 4635 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AatII 356 GACGTC GACGT^C
1982 GACGTC
AccI 3049 GTCTAC GT^[AC][GT]AC
4059 GTCTAC
AflII 2267 CTTAAG C^TTAAG
4186 CTTAAG
AflIII 831 ACGCGT A^C[GA][CT]GT
4901 ACGCGT
ApaI 129 GGGCCC GGGCC^C
294 GGGCCC
AspI 1336 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1409 GACATCGTC
AviII 4073 TGCGCA TGC^GCA
4142 TGCGCA
AvrII 2650 CCTAGG C^CTAGG
3014 CCTAGG
BalI 3416 TGGCCA TGG^CCA
4627 TGGCCA
BbrPI 746 CACGTG CAC^GTG
2693 CACGTG
BcgI 1768 CGACACAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3610 CGAACGATCTGC
BfrI 2267 CTTAAG C^TTAAG
4186 CTTAAG
BglI 182 GCCTCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
373 GCCTAGCGGGC
BglII 4362 AGATCT A^GATCT
5143 AGATCT
BlnI 2650 CCTAGG C^CTAGG
3014 CCTAGG
BlpI 1916 GCTGAGC GC^T[ACGT]AGC
3186 GCTCAGC
Bpu1102I 1916 GCTGAGC GC^T[ACGT]AGC
3186 GCTCAGC
BsaI 1334 GAGACC GGTCTC(1/5)
3466 GAGACC
BsaWI 2173 ACCGGT [AT]^CCGG[AT]
2348 ACCGGA
BsiEI 201 CGACCG CG[GA][CT]^CG
1374 CGACCG
BssHII 178 GCGCGC G^CGCGC
3289 GCGCGC
BstYI 4362 AGATCT [GA]^GATC[CT]
5143 AGATCT
CelII 1916 GCTGAGC GC^T[ACGT]AGC
3186 GCTCAGC
FspI 4073 TGCGCA TGC^GCA
4142 TGCGCA
MflI 4362 AGATCT [GA]^GATC[CT]
5143 AGATCT
MluI 831 ACGCGT A^CGCGT
4901 ACGCGT
MluNI 3416 TGGCCA TGG^CCA
4627 TGGCCA
MscI 3416 TGGCCA TGG^CCA
4627 TGGCCA
NspI 259 GCATGT [GA]CATG^[CT]
4635 GCATGC
PflFI 1336 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1409 GACATCGTC
PflMI 233 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2761 CCAGTATGTGG
PmaCI 746 CACGTG CAC^GTG
2693 CACGTG
PmlI 746 CACGTG CAC^GTG
2693 CACGTG
PshAI 1511 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3755 GACCTTGGTC
TaqI 1866 TCGA T^CGA
2072 TCGA
Tth111I 1336 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1409 GACATCGTC
TthHB8I 1866 TCGA T^CGA
2072 TCGA
Van91I 233 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2761 CCAGTATGTGG
XhoII 4362 AGATCT [GA]^GATC[CT]
5143 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV PleI
PpuMI PstI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI SspI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XmnI