##################################
# NO CUT ENZYMES (   65 enzymes) #
##################################

AccI        AccIII      AclI        AflII       AgeI        
AseI        AsnI        AviII       BclI        BfrI        
BlpI        Bpu1102I    BsaBI       BseAI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspLU11I    
BsrGI       Bst1107I    BstZ17I     CelII       ClaI        
CpoI        DraI        DraIII      EcoRV       EcoT22I     
FbaI        FspI        HincII      HindII      HindIII     
HpaI        MamI        MfeI        MluI        MroI        
MunI        NdeI        NotI        NruI        NsiI        
PacI        PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SalI        SbfI        
ScaI        SexAI       SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AatII        1683  GACGTC               GACGT^C
Acc65I         69  GGTACC               G^GTACC
AhdI         1318  GACACGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1024  GTGCAC               G^TGCAC
AocI         2450  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1024  GTGCAC               G^TGCAC
AscI          839  GGCGCGCC             GG^CGCGCC
Asp700I       864  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I        69  GGTACC               G^GTACC
AspEI        1318  GACACGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        3007  CCTAGG               C^CTAGG
BciVI        4151  GGATAC               GTATCC(6/5)
BglII        5019  AGATCT               A^GATCT
BlnI         3007  CCTAGG               C^CTAGG
BstBI        1603  TTCGAA               TT^CGAA
Bsu36I       2450  CCTGAGG              CC^T[ACGT]AGG
DrdI          970  GACTGCCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1318  GACACGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2450  CCTGAGG              CC^T[ACGT]AGG
EcoRI        3374  GAATTC               G^AATTC
FseI         1567  GGCCGGCC             GGCCGG^CC
KpnI           69  GGTACC               GGTAC^C
NaeI         1568  GCCGGC               GCC^GGC
NgoMIV       1568  GCCGGC               G^CCGGC
NheI         1446  GCTAGC               G^CTAGC
NspI          905  ACATGC               [GA]CATG^[CT]
NspV         1603  TTCGAA               TT^CGAA
PflMI        4955  CCAAACACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfuI         1603  TTCGAA               TT^CGAA
SspI         4319  AATATT               AAT^ATT
Van91I       4955  CCAAACACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          864  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI         3374  GAATTC               [GA]^AATT[CT]
             4487  AAATTC              
AflIII       2340  ACGTGT               A^C[GA][CT]GT
             4589  ACACGT              
ApoI         3374  GAATTC               [GA]^AATT[CT]
             4487  AAATTC              
AspI         1635  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2183  GACCTCGTC           
BamHI         503  GGATCC               G^GATCC
             1193  GGATCC              
BbsI          273  GAAGAC               GAAGAC(2/6)
             3112  GAAGAC              
BcgI         2119  CGAAGGCGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2251  GCACCCCGGTCG        
BpuAI         273  GAAGAC               GAAGAC(2/6)
             3112  GAAGAC              
BspHI         767  TCATGA               T^CATGA
             1370  TCATGA              
BstAPI        651  GCAGAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              900  GCAGGACATGC         
BstEII       4414  GGTGACC              G^GT[ACGT]ACC
             4441  GGTCACC             
BstPI        4414  GGTGACC              G^GT[ACGT]ACC
             4441  GGTCACC             
EcoNI        3137  CCTCCAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3263  CCTTCCCCAGG         
EcoO65I      4414  GGTGACC              G^GT[ACGT]ACC
             4441  GGTCACC             
MseI         3080  TTAA                 T^TAA
             4815  TTAA                
PaeR7I       2731  CTCGAG               C^TCGAG
             3968  CTCGAG              
PflFI        1635  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2183  GACCTCGTC           
PvuII         307  CAGCTG               CAG^CTG
             2772  CAGCTG              
RcaI          767  TCATGA               T^CATGA
             1370  TCATGA              
SapI          864  GAAGAGC              GCTCTTC(1/4)
             2398  GAAGAGC             
Tru9I        3080  TTAA                 T^TAA
             4815  TTAA                
Tth111I      1635  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2183  GACCTCGTC           
XhoI         2731  CTCGAG               C^TCGAG
             3968  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  138 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       Aor51HI     ApaI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbeI        BbrPI       BbvCI       BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BsaAI       
BsaHI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrDI       BsrFI       BsrI        
BssHII      BssKI       BssSI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EagI        EarI        EclXI       
Eco47III    Eco52I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     KspI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NarI        NciI        NcoI        
NdeII       NlaIII      NlaIV       PleI        PmaCI       
PmlI        PpuMI       PstI        RsaI        SacI        
SacII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfiI        SfoI        SmaI        SmlI        
StuI        StyI        TaqI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII       XmaCI       XmaI