##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
ApaI AscI Asp718I BamHI BanI
BbeI BbrPI BcgI BsaBI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BspLU11I BsrBI BssHII BssSI BstBI
BstEII BstPI ClaI CpoI DrdI
EagI EclXI Eco52I EcoNI EcoO65I
FseI HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NarI NheI NotI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 486 GTATAC GT^[AC][GT]AC
AflII 1789 CTTAAG C^TTAAG
AhdI 969 GACTTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3091 GTGCAC G^TGCAC
AocI 3179 CCTAAGG CC^T[ACGT]AGG
ApaLI 3091 GTGCAC G^TGCAC
AspEI 969 GACTTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 5059 CTCGGG C^[CT]CG[GA]G
AviII 2486 TGCGCA TGC^GCA
AvrII 800 CCTAGG C^CTAGG
BciVI 1749 GGATAC GTATCC(6/5)
BfrI 1789 CTTAAG C^TTAAG
BglII 394 AGATCT A^GATCT
BlnI 800 CCTAGG C^CTAGG
BsaI 1018 GAGACC GGTCTC(1/5)
BsmBI 4992 CGTCTC CGTCTC(1/5)
BsoBI 5059 CTCGGG C^[CT]CG[GA]G
BspHI 88 TCATGA T^CATGA
BsrFI 1541 GCCGGC [GA]^CCGG[CT]
Bst1107I 486 GTATAC GTA^TAC
BstZ17I 486 GTATAC GTA^TAC
Bsu36I 3179 CCTAAGG CC^T[ACGT]AGG
Cfr10I 1541 GCCGGC [GA]^CCGG[CT]
DraIII 3768 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 969 GACTTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3179 CCTAAGG CC^T[ACGT]AGG
EcoRV 1399 GATATC GAT^ATC
FspI 2486 TGCGCA TGC^GCA
HincII 1599 GTCAAC GT[CT]^[GA]AC
HindII 1599 GTCAAC GT[CT]^[GA]AC
NaeI 1541 GCCGGC GCC^GGC
NgoMIV 1541 GCCGGC G^CCGGC
NruI 4944 TCGCGA TCG^CGA
RcaI 88 TCATGA T^CATGA
SapI 3196 GCTCTTC GCTCTTC(1/4)
ScaI 2680 AGTACT AGT^ACT
SexAI 3002 ACCTGGT A^CC[AT]GGT
SfiI 3237 GGCCGCTATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 1880 AATATT AAT^ATT
StuI 3850 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 1100 GACGCC G[GA]^CG[CT]C
3291 GGCGTC
Aor51HI 942 AGCGCT AGC^GCT
4762 AGCGCT
AseI 4140 ATTAAT AT^TAAT
4253 ATTAAT
AsnI 4140 ATTAAT AT^TAAT
4253 ATTAAT
Asp700I 80 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
790 GAAGCTTTTC
AspI 3487 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5158 GACTCTGTC
BalI 2206 TGGCCA TGG^CCA
4972 TGGCCA
BanII 1607 GAGCTC G[GA]GC[CT]^C
3194 GAGCTC
BbsI 584 GAAGAC GAAGAC(2/6)
197 GTCTTC
BbvCI 4330 CCTCAGC CCTCAGC(-5/-2)
4929 GCTGAGG
BglI 954 GCCTTCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3238 GCCGCTATGGC
BpuAI 584 GAAGAC GAAGAC(2/6)
197 GTCTTC
BsaHI 1100 GACGCC G[GA]^CG[CT]C
3291 GGCGTC
Bsp1407I 2215 TGTACA T^GTACA
4752 TGTACA
BsrGI 2215 TGTACA T^GTACA
4752 TGTACA
Eco47III 942 AGCGCT AGC^GCT
4762 AGCGCT
FauI 1953 CCCGC CCCGC(4/6)
2969 CCCGC
Hin1I 1100 GACGCC G[GA]^CG[CT]C
3291 GGCGTC
MluNI 2206 TGGCCA TGG^CCA
4972 TGGCCA
MscI 2206 TGGCCA TGG^CCA
4972 TGGCCA
PflFI 3487 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5158 GACTCTGTC
PshBI 4140 ATTAAT AT^TAAT
4253 ATTAAT
SacI 1607 GAGCTC GAGCT^C
3194 GAGCTC
SspBI 2215 TGTACA T^GTACA
4752 TGTACA
Tth111I 3487 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5158 GACTCTGTC
XbaI 16 TCTAGA T^CTAGA
1065 TCTAGA
XcmI 3313 CCATGGCAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4685 CCATCCCATTACTGG
XmnI 80 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
790 GAAGCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BbvI BclI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRI EcoRII EcoT14I
EcoT22I FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeI NdeII NlaIII
NlaIV NsiI NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII